Identification and characterization of a pab gene cluster responsible for the 4-aminobenzoate degradation pathway, including its involvement in the formation of a γ-glutamylated intermediate in Paraburkholderia terrae strain KU-15
Copyright © 2023 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved..
Paraburkholderia terrae strain KU-15 grows on 2- and 4-nitrobenzoate and 2- and 4-aminobenzoate (ABA) as the sole nitrogen and carbon sources. The genes responsible for the potential degradation of 2- and 4-nitrobenzoate and 2-ABA have been predicted from its genome sequence. In this study, we identified the pab operon in P. terrae strain KU-15. This operon is responsible for the 4-ABA degradation pathway, which involves the formation of a γ-glutamylated intermediate. Reverse transcription-polymerase chain reaction revealed that the pab operon was induced by 4-ABA. Herein, studying the deletion of pabA and pabB1 in strain KU-15 and the examining of Escherichia coli expressing the pab operon revealed the involvement of the operon in 4-ABA degradation. The first step of the degradation pathway is the formation of a γ-glutamylated intermediate, whereby 4-ABA is converted to γ-glutamyl-4-carboxyanilide (γ-GCA). Subsequently, γ-GCA is oxidized to protocatechuate. Overexpression of various genes in E. coli and purification of recombinant proteins permitted the functional characterization of relevant pathway proteins: PabA is a γ-GCA synthetase, PabB1-B3 functions in a multicomponent dioxygenase system responsible for γ-GCA dioxygenation, and PabC is a γ-GCA hydrolase that reverses the formation of γ-GCA by PabA.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2024 2023 |
|---|---|
| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:137 |
|---|---|
| Enthalten in: |
Journal of bioscience and bioengineering - 137(2023), 1 vom: 01. Jan., Seite 38-46 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Liu, Yaxuan [VerfasserIn] |
|---|
| Links: |
|---|
| Anmerkungen: |
Date Completed 22.12.2023 Date Revised 22.12.2023 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1016/j.jbiosc.2023.11.002 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM364695102 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM364695102 | ||
| 003 | DE-627 | ||
| 005 | 20231227135940.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231226s2024 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.jbiosc.2023.11.002 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1235.xml |
| 035 | |a (DE-627)NLM364695102 | ||
| 035 | |a (NLM)37977976 | ||
| 035 | |a (PII)S1389-1723(23)00327-4 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Liu, Yaxuan |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Identification and characterization of a pab gene cluster responsible for the 4-aminobenzoate degradation pathway, including its involvement in the formation of a γ-glutamylated intermediate in Paraburkholderia terrae strain KU-15 |
| 264 | 1 | |c 2024 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 22.12.2023 | ||
| 500 | |a Date Revised 22.12.2023 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2023 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved. | ||
| 520 | |a Paraburkholderia terrae strain KU-15 grows on 2- and 4-nitrobenzoate and 2- and 4-aminobenzoate (ABA) as the sole nitrogen and carbon sources. The genes responsible for the potential degradation of 2- and 4-nitrobenzoate and 2-ABA have been predicted from its genome sequence. In this study, we identified the pab operon in P. terrae strain KU-15. This operon is responsible for the 4-ABA degradation pathway, which involves the formation of a γ-glutamylated intermediate. Reverse transcription-polymerase chain reaction revealed that the pab operon was induced by 4-ABA. Herein, studying the deletion of pabA and pabB1 in strain KU-15 and the examining of Escherichia coli expressing the pab operon revealed the involvement of the operon in 4-ABA degradation. The first step of the degradation pathway is the formation of a γ-glutamylated intermediate, whereby 4-ABA is converted to γ-glutamyl-4-carboxyanilide (γ-GCA). Subsequently, γ-GCA is oxidized to protocatechuate. Overexpression of various genes in E. coli and purification of recombinant proteins permitted the functional characterization of relevant pathway proteins: PabA is a γ-GCA synthetase, PabB1-B3 functions in a multicomponent dioxygenase system responsible for γ-GCA dioxygenation, and PabC is a γ-GCA hydrolase that reverses the formation of γ-GCA by PabA | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a 4-Aminobenzoate | |
| 650 | 4 | |a Aromatic amine compound | |
| 650 | 4 | |a Degradation pathway | |
| 650 | 4 | |a Glutamine synthetase-like protein | |
| 650 | 4 | |a Paraburkholderia terrae | |
| 650 | 4 | |a γ-Glutamylated intermediate | |
| 650 | 7 | |a para-Aminobenzoates |2 NLM | |
| 650 | 7 | |a 4-Aminobenzoic Acid |2 NLM | |
| 650 | 7 | |a TL2TJE8QTX |2 NLM | |
| 650 | 7 | |a 4-(2-anthryl)butanoic acid |2 NLM | |
| 650 | 7 | |a 73693-25-1 |2 NLM | |
| 650 | 7 | |a Nitrobenzoates |2 NLM | |
| 700 | 1 | |a Okano, Kenji |e verfasserin |4 aut | |
| 700 | 1 | |a Iwaki, Hiroaki |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Journal of bioscience and bioengineering |d 1999 |g 137(2023), 1 vom: 01. Jan., Seite 38-46 |w (DE-627)NLM158099222 |x 1347-4421 |7 nnns |
| 773 | 1 | 8 | |g volume:137 |g year:2023 |g number:1 |g day:01 |g month:01 |g pages:38-46 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1016/j.jbiosc.2023.11.002 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 137 |j 2023 |e 1 |b 01 |c 01 |h 38-46 | ||