Bacterial cGAS senses a viral RNA to initiate immunity
© 2023. The Author(s)..
Cyclic oligonucleotide-based antiphage signalling systems (CBASS) protect prokaryotes from viral (phage) attack through the production of cyclic oligonucleotides, which activate effector proteins that trigger the death of the infected host1,2. How bacterial cyclases recognize phage infection is not known. Here we show that staphylococcal phages produce a structured RNA transcribed from the terminase subunit genes, termed CBASS-activating bacteriophage RNA (cabRNA), which binds to a positively charged surface of the CdnE03 cyclase and promotes the synthesis of the cyclic dinucleotide cGAMP to activate the CBASS immune response. Phages that escape the CBASS defence harbour mutations that lead to the generation of a longer form of the cabRNA that cannot activate CdnE03. As the mammalian cyclase OAS1 also binds viral double-stranded RNA during the interferon response, our results reveal a conserved mechanism for the activation of innate antiviral defence pathways.
Errataetall: | |
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Medienart: |
E-Artikel |
Erscheinungsjahr: |
2023 |
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Erschienen: |
2023 |
Enthalten in: |
Zur Gesamtaufnahme - volume:623 |
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Enthalten in: |
Nature - 623(2023), 7989 vom: 15. Nov., Seite 1001-1008 |
Sprache: |
Englisch |
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Beteiligte Personen: |
Banh, Dalton V [VerfasserIn] |
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Links: |
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Themen: |
2',5'-Oligoadenylate Synthetase |
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Anmerkungen: |
Date Completed 04.12.2023 Date Revised 12.12.2023 published: Print-Electronic ErratumIn: Nature. 2023 Dec 5;:. - PMID 38052939 Citation Status MEDLINE |
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doi: |
10.1038/s41586-023-06743-9 |
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funding: |
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PPN (Katalog-ID): |
NLM36459974X |
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520 | |a Cyclic oligonucleotide-based antiphage signalling systems (CBASS) protect prokaryotes from viral (phage) attack through the production of cyclic oligonucleotides, which activate effector proteins that trigger the death of the infected host1,2. How bacterial cyclases recognize phage infection is not known. Here we show that staphylococcal phages produce a structured RNA transcribed from the terminase subunit genes, termed CBASS-activating bacteriophage RNA (cabRNA), which binds to a positively charged surface of the CdnE03 cyclase and promotes the synthesis of the cyclic dinucleotide cGAMP to activate the CBASS immune response. Phages that escape the CBASS defence harbour mutations that lead to the generation of a longer form of the cabRNA that cannot activate CdnE03. As the mammalian cyclase OAS1 also binds viral double-stranded RNA during the interferon response, our results reveal a conserved mechanism for the activation of innate antiviral defence pathways | ||
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