Genotypic characterization of Streptococcus didelphis causative of fatal infection in white-eared opossums
© The Author(s) 2023. Published by Oxford University Press on behalf of Applied Microbiology International..
Streptococcus didelphis was once reported as related to severe infections in opossums. Thus, we present the first comprehensive whole-genome characterization of clinical S. didelphis strains isolated from white-eared opossums (Didelphis albiventris). Long-read whole-genome sequencing was performed using the MinION platform, which allowed the prediction of several genomic features. We observed that S. didelphis genomes harbor a cluster for streptolysin biosynthesis and a conserved genomic island with genes involved in transcriptional regulation (arlR) and transmembrane transport (bcrA). Antimicrobial resistance genes for several drug classes were found, including beta-lactam, which is the main antimicrobial class used in Streptococcus spp. infections; however, no phenotypical resistance was observed. In addition, we predicted the presence of 33 virulence factors in the analyzed genomes. High phylogenetic similarity was observed between clinical and reference strains, yet no clonality was suggested. We also proposed dnaN, gki, pros, and xpt as housekeeping candidates to be used in S. didelphis sequence typing. This is the first whole-genome characterization of S. didelphis, whose data provide important insights into its pathogenicity.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:76 |
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| Enthalten in: |
Letters in applied microbiology - 76(2023), 12 vom: 07. Dez. |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Breyer, Gabriela Merker [VerfasserIn] |
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| Links: |
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| Themen: |
Anti-Infective Agents |
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| Anmerkungen: |
Date Completed 16.12.2023 Date Revised 16.12.2023 published: Print Citation Status MEDLINE |
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| doi: |
10.1093/lambio/ovad131 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 520 | |a © The Author(s) 2023. Published by Oxford University Press on behalf of Applied Microbiology International. | ||
| 520 | |a Streptococcus didelphis was once reported as related to severe infections in opossums. Thus, we present the first comprehensive whole-genome characterization of clinical S. didelphis strains isolated from white-eared opossums (Didelphis albiventris). Long-read whole-genome sequencing was performed using the MinION platform, which allowed the prediction of several genomic features. We observed that S. didelphis genomes harbor a cluster for streptolysin biosynthesis and a conserved genomic island with genes involved in transcriptional regulation (arlR) and transmembrane transport (bcrA). Antimicrobial resistance genes for several drug classes were found, including beta-lactam, which is the main antimicrobial class used in Streptococcus spp. infections; however, no phenotypical resistance was observed. In addition, we predicted the presence of 33 virulence factors in the analyzed genomes. High phylogenetic similarity was observed between clinical and reference strains, yet no clonality was suggested. We also proposed dnaN, gki, pros, and xpt as housekeeping candidates to be used in S. didelphis sequence typing. This is the first whole-genome characterization of S. didelphis, whose data provide important insights into its pathogenicity | ||
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| 700 | 1 | |a Rocha Jacques da Silva, Maria Eduarda |e verfasserin |4 aut | |
| 700 | 1 | |a Slaviero, Mônica |e verfasserin |4 aut | |
| 700 | 1 | |a Albuquerque de Almeida, Bruno |e verfasserin |4 aut | |
| 700 | 1 | |a Machado Sousa da Silva, Emanoelly |e verfasserin |4 aut | |
| 700 | 1 | |a de Queiroz Schmidt, Victória Regina |e verfasserin |4 aut | |
| 700 | 1 | |a Alievi, Marcelo |e verfasserin |4 aut | |
| 700 | 1 | |a Maboni, Grazieli |e verfasserin |4 aut | |
| 700 | 1 | |a Petinatti Pavarini, Saulo |e verfasserin |4 aut | |
| 700 | 1 | |a Maboni Siqueira, Franciele |e verfasserin |4 aut | |
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