Use of Localized Reconstruction to Visualize the Shigella Phage Sf6 Tail Apparatus
© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature..
Cryogenic electron microscopy (cryo-EM) single-particle analysis has revolutionized the structural analysis of icosahedral viruses, including tailed bacteriophages. In recent years, localized (or focused) reconstruction has emerged as a powerful data analysis method to capture symmetry mismatches and resolve asymmetric features in icosahedral viruses. Here, we describe the methods used to reconstruct the 2.65-MDa tail apparatus of the Shigella phage Sf6, a representative member of the Podoviridae superfamily.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:2738 |
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| Enthalten in: |
Methods in molecular biology (Clifton, N.J.) - 2738(2024) vom: 01., Seite 215-228 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Hou, Chun-Feng David [VerfasserIn] |
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| Links: |
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| Themen: |
Bacteriophages |
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| Anmerkungen: |
Date Completed 16.11.2023 Date Revised 02.01.2024 published: Print Citation Status MEDLINE |
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| doi: |
10.1007/978-1-0716-3549-0_14 |
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| 520 | |a Cryogenic electron microscopy (cryo-EM) single-particle analysis has revolutionized the structural analysis of icosahedral viruses, including tailed bacteriophages. In recent years, localized (or focused) reconstruction has emerged as a powerful data analysis method to capture symmetry mismatches and resolve asymmetric features in icosahedral viruses. Here, we describe the methods used to reconstruct the 2.65-MDa tail apparatus of the Shigella phage Sf6, a representative member of the Podoviridae superfamily | ||
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