Next-generation genetic sexing strain establishment in the agricultural pest Ceratitis capitata
© 2023. Crown..
Tephritid fruit fly pests pose an increasing threat to the agricultural industry due to their global dispersion and a highly invasive nature. Here we showcase the feasibility of an early-detection SEPARATOR sex sorting approach through using the non-model Tephritid pest, Ceratitis capitata. This system relies on female-only fluorescent marker expression, accomplished through the use of a sex-specific intron of the highly-conserved transformer gene from C. capitata and Anastrepha ludens. The herein characterized strains have 100% desired phenotype outcomes, allowing accurate male-female separation during early development. Overall, we describe an antibiotic and temperature-independent sex-sorting system in C. capitata, which, moving forward, may be implemented in other non-model Tephritid pest species. This strategy can facilitate the establishment of genetic sexing systems with endogenous elements exclusively, which, on a wider scale, can improve pest population control strategies like sterile insect technique.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:13 |
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| Enthalten in: |
Scientific reports - 13(2023), 1 vom: 14. Nov., Seite 19866 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Davydova, Serafima [VerfasserIn] |
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| Links: |
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| Themen: |
Journal Article |
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| Anmerkungen: |
Date Completed 16.11.2023 Date Revised 24.11.2023 published: Electronic Citation Status MEDLINE |
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| doi: |
10.1038/s41598-023-47276-5 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM364557699 |
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| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2023. Crown. | ||
| 520 | |a Tephritid fruit fly pests pose an increasing threat to the agricultural industry due to their global dispersion and a highly invasive nature. Here we showcase the feasibility of an early-detection SEPARATOR sex sorting approach through using the non-model Tephritid pest, Ceratitis capitata. This system relies on female-only fluorescent marker expression, accomplished through the use of a sex-specific intron of the highly-conserved transformer gene from C. capitata and Anastrepha ludens. The herein characterized strains have 100% desired phenotype outcomes, allowing accurate male-female separation during early development. Overall, we describe an antibiotic and temperature-independent sex-sorting system in C. capitata, which, moving forward, may be implemented in other non-model Tephritid pest species. This strategy can facilitate the establishment of genetic sexing systems with endogenous elements exclusively, which, on a wider scale, can improve pest population control strategies like sterile insect technique | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, U.S. Gov't, Non-P.H.S. | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 700 | 1 | |a Liu, Junru |e verfasserin |4 aut | |
| 700 | 1 | |a Kandul, Nikolay P |e verfasserin |4 aut | |
| 700 | 1 | |a Braswell, W Evan |e verfasserin |4 aut | |
| 700 | 1 | |a Akbari, Omar S |e verfasserin |4 aut | |
| 700 | 1 | |a Meccariello, Angela |e verfasserin |4 aut | |
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