Target-Guided Isolation and Purification of Antioxidants from Urtica laetevirens Maxim. by HSCCC Combined with Online DPPH-HPLC Analysis
Urtica laetevirens Maxim. is used extensively in traditional Chinese medicine (TCM) for its potent antioxidative properties. In this study, three antioxidants were purified from U. laetevirens. using HSCCC guided by online DPPH-HPLC analysis. Firstly, the online DPPH-HPLC analysis was performed to profile out the antioxidant active molecules in U. laetevirens. The ultrasonic-assisted extraction conditions were optimized by response surface methodology and the results showed the targeted antioxidant active molecules could be well enriched under the optimized extraction conditions. Then, the antioxidant active molecules were separated by high-speed countercurrent chromatography ethyl acetate/n-butanol/water (2:3:5, v/v/v) as the solvent system. Finally, the three targets including 16.8 mg of Isovitexin, 9.8 mg of Isoorientin, and 26.7 mg of Apigenin-6,8-di-C-β-d-glucopyranoside were obtained from 100 mg of sample. Their structures were identified by 1H NMR spectroscopy.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:28 |
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| Enthalten in: |
Molecules (Basel, Switzerland) - 28(2023), 21 vom: 29. Okt. |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Li, Aijing [VerfasserIn] |
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| Links: |
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| Themen: |
Antioxidant active molecules |
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| Anmerkungen: |
Date Completed 15.11.2023 Date Revised 17.11.2023 published: Electronic Citation Status MEDLINE |
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| doi: |
10.3390/molecules28217332 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM364513918 |
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| 500 | |a Date Revised 17.11.2023 | ||
| 500 | |a published: Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Urtica laetevirens Maxim. is used extensively in traditional Chinese medicine (TCM) for its potent antioxidative properties. In this study, three antioxidants were purified from U. laetevirens. using HSCCC guided by online DPPH-HPLC analysis. Firstly, the online DPPH-HPLC analysis was performed to profile out the antioxidant active molecules in U. laetevirens. The ultrasonic-assisted extraction conditions were optimized by response surface methodology and the results showed the targeted antioxidant active molecules could be well enriched under the optimized extraction conditions. Then, the antioxidant active molecules were separated by high-speed countercurrent chromatography ethyl acetate/n-butanol/water (2:3:5, v/v/v) as the solvent system. Finally, the three targets including 16.8 mg of Isovitexin, 9.8 mg of Isoorientin, and 26.7 mg of Apigenin-6,8-di-C-β-d-glucopyranoside were obtained from 100 mg of sample. Their structures were identified by 1H NMR spectroscopy | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a HSCCC | |
| 650 | 4 | |a Urtica laetevirens Maxim. | |
| 650 | 4 | |a antioxidant active molecules | |
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| 700 | 1 | |a La, Mencuo |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Huichun |e verfasserin |4 aut | |
| 700 | 1 | |a Zhao, Jianzhong |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Yao |e verfasserin |4 aut | |
| 700 | 1 | |a Mian, Ruisha |e verfasserin |4 aut | |
| 700 | 1 | |a He, Fangfang |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Yuhan |e verfasserin |4 aut | |
| 700 | 1 | |a Yang, Tingqin |e verfasserin |4 aut | |
| 700 | 1 | |a Zou, Denglang |e verfasserin |4 aut | |
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