Identification and Validation of Glomeruli Cellular Senescence-Related Genes in Diabetic Nephropathy by Multiomics
© 2023 Wiley-VCH GmbH..
Accumulating evidence indicates that cellular premature senescence of the glomerulus, including endothelial cells, mesangial cells, and podocytes leads to diabetic nephropathy (DN), and DN is regarded as a clinical model of premature senescence. However, the role of cellular senescence-associated genes in the glomerulus in DN progression remains unclear. Therefore, this work aims to identify and validate potential cellular aging-related genes in the glomerulus in DN to provide novel clues for DN treatment based on anti-aging. The microarray GSE96804 dataset, including 41 diabetic glomeruli and 20 control glomeruli, is retrieved from the Gene Expression Omnibus (GEO) database and cellular senescence-related genes (CSRGs) are obtained from the GeneCards database and literature reports. Subsequently, PPI, GO, and KEGG enrichment are analyzed by screening the intersection between differentially expressed genes (DEGs) and CSRGs. scRNA-seq dataset GSE127235 is used to verify core genes expression in glomerulocytes of mice. Finally, db/db mice are utilized to validate the hub gene expression in the glomeruli, and high glucose-induced mesangial cells are used to confirm key gene expression. This study reveals that FOS and ZFP36 may play an anti-aging role in DN to ameliorate cell intracellular premature aging in mesangial cells of glomeruli.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2024 |
|---|---|
| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:8 |
|---|---|
| Enthalten in: |
Advanced biology - 8(2024), 2 vom: 13. Feb., Seite e2300453 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Tang, Chunyin [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
Anti-aging |
|---|
| Anmerkungen: |
Date Completed 14.02.2024 Date Revised 14.02.2024 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1002/adbi.202300453 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM364491795 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM364491795 | ||
| 003 | DE-627 | ||
| 005 | 20240214232920.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231226s2024 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1002/adbi.202300453 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1293.xml |
| 035 | |a (DE-627)NLM364491795 | ||
| 035 | |a (NLM)37957539 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Tang, Chunyin |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Identification and Validation of Glomeruli Cellular Senescence-Related Genes in Diabetic Nephropathy by Multiomics |
| 264 | 1 | |c 2024 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 14.02.2024 | ||
| 500 | |a Date Revised 14.02.2024 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2023 Wiley-VCH GmbH. | ||
| 520 | |a Accumulating evidence indicates that cellular premature senescence of the glomerulus, including endothelial cells, mesangial cells, and podocytes leads to diabetic nephropathy (DN), and DN is regarded as a clinical model of premature senescence. However, the role of cellular senescence-associated genes in the glomerulus in DN progression remains unclear. Therefore, this work aims to identify and validate potential cellular aging-related genes in the glomerulus in DN to provide novel clues for DN treatment based on anti-aging. The microarray GSE96804 dataset, including 41 diabetic glomeruli and 20 control glomeruli, is retrieved from the Gene Expression Omnibus (GEO) database and cellular senescence-related genes (CSRGs) are obtained from the GeneCards database and literature reports. Subsequently, PPI, GO, and KEGG enrichment are analyzed by screening the intersection between differentially expressed genes (DEGs) and CSRGs. scRNA-seq dataset GSE127235 is used to verify core genes expression in glomerulocytes of mice. Finally, db/db mice are utilized to validate the hub gene expression in the glomeruli, and high glucose-induced mesangial cells are used to confirm key gene expression. This study reveals that FOS and ZFP36 may play an anti-aging role in DN to ameliorate cell intracellular premature aging in mesangial cells of glomeruli | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Cellular senescence | |
| 650 | 4 | |a anti-aging | |
| 650 | 4 | |a diabetic nephropathy | |
| 650 | 4 | |a glomerulus | |
| 650 | 4 | |a multiomics | |
| 700 | 1 | |a Yang, Chunsong |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Peiwen |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Luxin |e verfasserin |4 aut | |
| 700 | 1 | |a Lin, Yunzhu |e verfasserin |4 aut | |
| 700 | 1 | |a Yi, Qiusha |e verfasserin |4 aut | |
| 700 | 1 | |a Tang, Fengru |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Lantao |e verfasserin |4 aut | |
| 700 | 1 | |a Zhou, Wei |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Dongwen |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Lingli |e verfasserin |4 aut | |
| 700 | 1 | |a Yuan, Xiaohuan |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Advanced biology |d 2021 |g 8(2024), 2 vom: 13. Feb., Seite e2300453 |w (DE-627)NLM314964096 |x 2701-0198 |7 nnns |
| 773 | 1 | 8 | |g volume:8 |g year:2024 |g number:2 |g day:13 |g month:02 |g pages:e2300453 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1002/adbi.202300453 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 8 |j 2024 |e 2 |b 13 |c 02 |h e2300453 | ||