Angiotensin II type 1 receptor-associated protein deletion combined with angiotensin II stimulation accelerates the development of diabetic kidney disease in mice on a C57BL/6 strain
© 2023. The Author(s), under exclusive licence to The Japanese Society of Hypertension..
The progress in the research field of diabetic kidney disease (DKD) has been disturbed by the lack of reliable animal models. Angiotensin II (Ang II) type 1 receptor (AT1R)-associated protein (ATRAP) promotes internalization of AT1R and selectively inhibits pathological AT1R signaling. In this study, we investigated whether overactivation of the renin-angiotensin system (RAS) through a combination of ATRAP deletion with Ang II stimulation developed a progressive DKD model in C57BL/6 mice, which are resistant to the development of kidney injury. Eight-week-old male systemic ATRAP-knockout mice on the C57BL/6 strain (KO) and their littermate wild-type mice (Ctrl) were divided into five groups: 1) Ctrl, 2) Ctrl-streptozotocin (STZ), 3) KO-STZ, 4) Ctrl-STZ-Ang II, and 5) KO-STZ-Ang II. Ang II was administered for 6 weeks from 4 weeks after STZ administration. At 10 weeks after STZ administration, mice were euthanized to evaluate kidney injuries. Neither ATRAP deletion alone nor Ang II stimulation alone developed a progressive DKD model in STZ-induced diabetic C57BL/6 mice. However, a combination of ATRAP deletion with Ang II stimulation accelerated the development of DKD as manifested by overt albuminuria, glomerular hypertrophy, podocyte loss, mesangial expansion, kidney interstitial fibrosis and functional insufficiency, concomitant with increased angiotensinogen and AT1R expression in the kidneys. In STZ-induced diabetic C57BL/6 mice that are resistant to the development of kidney injury, the combination of ATRAP deletion and Ang II stimulation accelerates the development of DKD, which may be associated with intrarenal RAS overactivation.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:47 |
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| Enthalten in: |
Hypertension research : official journal of the Japanese Society of Hypertension - 47(2024), 1 vom: 20. Jan., Seite 55-66 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Taguchi, Shinya [VerfasserIn] |
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| Links: |
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| Themen: |
11128-99-7 |
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| Anmerkungen: |
Date Completed 08.01.2024 Date Revised 16.01.2024 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1038/s41440-023-01496-4 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM364488824 |
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| 100 | 1 | |a Taguchi, Shinya |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Angiotensin II type 1 receptor-associated protein deletion combined with angiotensin II stimulation accelerates the development of diabetic kidney disease in mice on a C57BL/6 strain |
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| 520 | |a © 2023. The Author(s), under exclusive licence to The Japanese Society of Hypertension. | ||
| 520 | |a The progress in the research field of diabetic kidney disease (DKD) has been disturbed by the lack of reliable animal models. Angiotensin II (Ang II) type 1 receptor (AT1R)-associated protein (ATRAP) promotes internalization of AT1R and selectively inhibits pathological AT1R signaling. In this study, we investigated whether overactivation of the renin-angiotensin system (RAS) through a combination of ATRAP deletion with Ang II stimulation developed a progressive DKD model in C57BL/6 mice, which are resistant to the development of kidney injury. Eight-week-old male systemic ATRAP-knockout mice on the C57BL/6 strain (KO) and their littermate wild-type mice (Ctrl) were divided into five groups: 1) Ctrl, 2) Ctrl-streptozotocin (STZ), 3) KO-STZ, 4) Ctrl-STZ-Ang II, and 5) KO-STZ-Ang II. Ang II was administered for 6 weeks from 4 weeks after STZ administration. At 10 weeks after STZ administration, mice were euthanized to evaluate kidney injuries. Neither ATRAP deletion alone nor Ang II stimulation alone developed a progressive DKD model in STZ-induced diabetic C57BL/6 mice. However, a combination of ATRAP deletion with Ang II stimulation accelerated the development of DKD as manifested by overt albuminuria, glomerular hypertrophy, podocyte loss, mesangial expansion, kidney interstitial fibrosis and functional insufficiency, concomitant with increased angiotensinogen and AT1R expression in the kidneys. In STZ-induced diabetic C57BL/6 mice that are resistant to the development of kidney injury, the combination of ATRAP deletion and Ang II stimulation accelerates the development of DKD, which may be associated with intrarenal RAS overactivation | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Angiotensin receptors | |
| 650 | 4 | |a Animal models | |
| 650 | 4 | |a Diabetic kidney disease | |
| 650 | 4 | |a Renin-angiotensin system | |
| 650 | 7 | |a Angiotensin II |2 NLM | |
| 650 | 7 | |a 11128-99-7 |2 NLM | |
| 650 | 7 | |a Receptor, Angiotensin, Type 1 |2 NLM | |
| 650 | 7 | |a Adaptor Proteins, Signal Transducing |2 NLM | |
| 700 | 1 | |a Azushima, Kengo |e verfasserin |4 aut | |
| 700 | 1 | |a Yamaji, Takahiro |e verfasserin |4 aut | |
| 700 | 1 | |a Suzuki, Toru |e verfasserin |4 aut | |
| 700 | 1 | |a Abe, Eriko |e verfasserin |4 aut | |
| 700 | 1 | |a Tanaka, Shohei |e verfasserin |4 aut | |
| 700 | 1 | |a Hirota, Keigo |e verfasserin |4 aut | |
| 700 | 1 | |a Tsukamoto, Shunichiro |e verfasserin |4 aut | |
| 700 | 1 | |a Morita, Ryutaro |e verfasserin |4 aut | |
| 700 | 1 | |a Kobayashi, Ryu |e verfasserin |4 aut | |
| 700 | 1 | |a Kinguchi, Sho |e verfasserin |4 aut | |
| 700 | 1 | |a Yamashita, Akio |e verfasserin |4 aut | |
| 700 | 1 | |a Wakui, Hiromichi |e verfasserin |4 aut | |
| 700 | 1 | |a Tamura, Kouichi |e verfasserin |4 aut | |
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