tRNA m1G9 modification depends on substrate-specific RNA conformational changes induced by the methyltransferase Trm10
Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved..
The methyltransferase Trm10 modifies a subset of tRNAs on the base N1 position of the ninth nucleotide in the tRNA core. Trm10 is conserved throughout Eukarya and Archaea, and mutations in the human gene (TRMT10A) have been linked to neurological disorders such as microcephaly and intellectual disability, as well as defects in glucose metabolism. Of the 26 tRNAs in yeast with guanosine at position 9, only 13 are substrates for Trm10. However, no common sequence or other posttranscriptional modifications have been identified among these substrates, suggesting the presence of some other tRNA feature(s) that allow Trm10 to distinguish substrate from nonsubstrate tRNAs. Here, we show that substrate recognition by Saccharomyces cerevisiae Trm10 is dependent on both intrinsic tRNA flexibility and the ability of the enzyme to induce specific tRNA conformational changes upon binding. Using the sensitive RNA structure-probing method SHAPE, conformational changes upon binding to Trm10 in tRNA substrates, but not nonsubstrates, were identified and mapped onto a model of Trm10-bound tRNA. These changes may play an important role in substrate recognition by allowing Trm10 to gain access to the target nucleotide. Our results highlight a novel mechanism of substrate recognition by a conserved tRNA modifying enzyme. Further, these studies reveal a strategy for substrate recognition that may be broadly employed by tRNA-modifying enzymes which must distinguish between structurally similar tRNA species.
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| Medienart: |
E-Artikel |
| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:299 |
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| Enthalten in: |
The Journal of biological chemistry - 299(2023), 12 vom: 10. Dez., Seite 105443 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Strassler, Sarah E [VerfasserIn] |
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| Anmerkungen: |
Date Completed 29.01.2024 Date Revised 29.01.2024 published: Print-Electronic UpdateOf: bioRxiv. 2023 Oct 19;:. - PMID 36778341 Citation Status MEDLINE |
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| doi: |
10.1016/j.jbc.2023.105443 |
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| Förderinstitution / Projekttitel: |
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NLM364408855 |
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| 100 | 1 | |a Strassler, Sarah E |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a tRNA m1G9 modification depends on substrate-specific RNA conformational changes induced by the methyltransferase Trm10 |
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| 500 | |a UpdateOf: bioRxiv. 2023 Oct 19;:. - PMID 36778341 | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved. | ||
| 520 | |a The methyltransferase Trm10 modifies a subset of tRNAs on the base N1 position of the ninth nucleotide in the tRNA core. Trm10 is conserved throughout Eukarya and Archaea, and mutations in the human gene (TRMT10A) have been linked to neurological disorders such as microcephaly and intellectual disability, as well as defects in glucose metabolism. Of the 26 tRNAs in yeast with guanosine at position 9, only 13 are substrates for Trm10. However, no common sequence or other posttranscriptional modifications have been identified among these substrates, suggesting the presence of some other tRNA feature(s) that allow Trm10 to distinguish substrate from nonsubstrate tRNAs. Here, we show that substrate recognition by Saccharomyces cerevisiae Trm10 is dependent on both intrinsic tRNA flexibility and the ability of the enzyme to induce specific tRNA conformational changes upon binding. Using the sensitive RNA structure-probing method SHAPE, conformational changes upon binding to Trm10 in tRNA substrates, but not nonsubstrates, were identified and mapped onto a model of Trm10-bound tRNA. These changes may play an important role in substrate recognition by allowing Trm10 to gain access to the target nucleotide. Our results highlight a novel mechanism of substrate recognition by a conserved tRNA modifying enzyme. Further, these studies reveal a strategy for substrate recognition that may be broadly employed by tRNA-modifying enzymes which must distinguish between structurally similar tRNA species | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a RNA methylation | |
| 650 | 4 | |a RNA structure | |
| 650 | 4 | |a SHAPE RNA structure probing | |
| 650 | 4 | |a SPOUT methyltransferase | |
| 650 | 4 | |a substrate recognition | |
| 650 | 4 | |a transfer RNA (tRNA) | |
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| 650 | 7 | |a tRNA Methyltransferases |2 NLM | |
| 650 | 7 | |a EC 2.1.1.- |2 NLM | |
| 700 | 1 | |a Bowles, Isobel E |e verfasserin |4 aut | |
| 700 | 1 | |a Krishnamohan, Aiswarya |e verfasserin |4 aut | |
| 700 | 1 | |a Kim, Hyejeong |e verfasserin |4 aut | |
| 700 | 1 | |a Edgington, Catherine B |e verfasserin |4 aut | |
| 700 | 1 | |a Kuiper, Emily G |e verfasserin |4 aut | |
| 700 | 1 | |a Hancock, Clio J |e verfasserin |4 aut | |
| 700 | 1 | |a Comstock, Lindsay R |e verfasserin |4 aut | |
| 700 | 1 | |a Jackman, Jane E |e verfasserin |4 aut | |
| 700 | 1 | |a Conn, Graeme L |e verfasserin |4 aut | |
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| 856 | 4 | 0 | |u http://dx.doi.org/10.1016/j.jbc.2023.105443 |3 Volltext |
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