Single-Cell Profiling of Bone Marrow B Cells and Early B Cell Developmental Disorders Associated With Systemic Lupus Erythematosus
© 2023 The Authors. Arthritis & Rheumatology published by Wiley Periodicals LLC on behalf of American College of Rheumatology..
OBJECTIVE: The peripheral B cell compartment is heavily disturbed in systemic lupus erythematosus (SLE), but whether B cells develop aberrantly in the bone marrow (BM) is largely unknown.
METHODS: We performed single-cell RNA/B cell receptor (BCR) sequencing and immune profiling of BM B cells and classified patients with SLE into two groups: early B cell (Pro-B and Pre-B) normal (EBnor) and EB defective/low (EBlo) groups.
RESULTS: The SLE-EBlo group exhibited more severe disease activity and proinflammatory status, overaction of type I interferon signaling and metabolic pathways within the B cell compartment, and aberrant BCR repertoires compared with the SLE-EBnor group. Moreover, in one patient with SLE who was initially classified in the SLE-EBlo group, early B cell deficiency and associated abnormalities were largely rectified in a second BM sample at the remission phase.
CONCLUSION: In summary, this study suggests that early B cell loss in BM defines a unique pathological state in a subset of patients with SLE that may play an active role in the dysregulated autoimmune responses.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:76 |
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| Enthalten in: |
Arthritis & rheumatology (Hoboken, N.J.) - 76(2024), 4 vom: 27. März, Seite 599-613 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Dong, Chen [VerfasserIn] |
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| Anmerkungen: |
Date Completed 28.03.2024 Date Revised 28.03.2024 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1002/art.42750 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM364383356 |
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| 245 | 1 | 0 | |a Single-Cell Profiling of Bone Marrow B Cells and Early B Cell Developmental Disorders Associated With Systemic Lupus Erythematosus |
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| 500 | |a Date Completed 28.03.2024 | ||
| 500 | |a Date Revised 28.03.2024 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2023 The Authors. Arthritis & Rheumatology published by Wiley Periodicals LLC on behalf of American College of Rheumatology. | ||
| 520 | |a OBJECTIVE: The peripheral B cell compartment is heavily disturbed in systemic lupus erythematosus (SLE), but whether B cells develop aberrantly in the bone marrow (BM) is largely unknown | ||
| 520 | |a METHODS: We performed single-cell RNA/B cell receptor (BCR) sequencing and immune profiling of BM B cells and classified patients with SLE into two groups: early B cell (Pro-B and Pre-B) normal (EBnor) and EB defective/low (EBlo) groups | ||
| 520 | |a RESULTS: The SLE-EBlo group exhibited more severe disease activity and proinflammatory status, overaction of type I interferon signaling and metabolic pathways within the B cell compartment, and aberrant BCR repertoires compared with the SLE-EBnor group. Moreover, in one patient with SLE who was initially classified in the SLE-EBlo group, early B cell deficiency and associated abnormalities were largely rectified in a second BM sample at the remission phase | ||
| 520 | |a CONCLUSION: In summary, this study suggests that early B cell loss in BM defines a unique pathological state in a subset of patients with SLE that may play an active role in the dysregulated autoimmune responses | ||
| 650 | 4 | |a Journal Article | |
| 700 | 1 | |a Guo, Yicheng |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Zechuan |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Teng |e verfasserin |4 aut | |
| 700 | 1 | |a Ji, Juan |e verfasserin |4 aut | |
| 700 | 1 | |a Sun, Chi |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Jing |e verfasserin |4 aut | |
| 700 | 1 | |a Cao, Haixia |e verfasserin |4 aut | |
| 700 | 1 | |a Xia, Yunfei |e verfasserin |4 aut | |
| 700 | 1 | |a Xue, Zhonghui |e verfasserin |4 aut | |
| 700 | 1 | |a Gu, Xixi |e verfasserin |4 aut | |
| 700 | 1 | |a Liang, Qian |e verfasserin |4 aut | |
| 700 | 1 | |a Zhao, Rui |e verfasserin |4 aut | |
| 700 | 1 | |a Fu, Ting |e verfasserin |4 aut | |
| 700 | 1 | |a Ma, Jiaqiang |e verfasserin |4 aut | |
| 700 | 1 | |a Jiang, Shan |e verfasserin |4 aut | |
| 700 | 1 | |a Wu, Chunmei |e verfasserin |4 aut | |
| 700 | 1 | |a Fu, Qiong |e verfasserin |4 aut | |
| 700 | 1 | |a Guo, Genkai |e verfasserin |4 aut | |
| 700 | 1 | |a Bao, Yanfeng |e verfasserin |4 aut | |
| 700 | 1 | |a Guo, Hua |e verfasserin |4 aut | |
| 700 | 1 | |a Yang, Junling |e verfasserin |4 aut | |
| 700 | 1 | |a Xu, Min |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Xiaoming |e verfasserin |4 aut | |
| 700 | 1 | |a Sheng, Zizhang |e verfasserin |4 aut | |
| 700 | 1 | |a Gu, Zhifeng |e verfasserin |4 aut | |
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