Release of Histone H3K4-reading transcription factors from chromosomes in mitosis is independent of adjacent H3 phosphorylation
© 2023. The Author(s)..
Histone modifications influence the recruitment of reader proteins to chromosomes to regulate events including transcription and cell division. The idea of a histone code, where combinations of modifications specify unique downstream functions, is widely accepted and can be demonstrated in vitro. For example, on synthetic peptides, phosphorylation of Histone H3 at threonine-3 (H3T3ph) prevents the binding of reader proteins that recognize trimethylation of the adjacent lysine-4 (H3K4me3), including the TAF3 component of TFIID. To study these combinatorial effects in cells, we analyzed the genome-wide distribution of H3T3ph and H3K4me2/3 during mitosis. We find that H3T3ph anti-correlates with adjacent H3K4me2/3 in cells, and that the PHD domain of TAF3 can bind H3K4me2/3 in isolated mitotic chromatin despite the presence of H3T3ph. Unlike in vitro, H3K4 readers are still displaced from chromosomes in mitosis in Haspin-depleted cells lacking H3T3ph. H3T3ph is therefore unlikely to be responsible for transcriptional downregulation during cell division.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:14 |
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| Enthalten in: |
Nature communications - 14(2023), 1 vom: 09. Nov., Seite 7243 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Harris, Rebecca J [VerfasserIn] |
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| Links: |
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| Themen: |
Histones |
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| Anmerkungen: |
Date Completed 13.11.2023 Date Revised 10.02.2024 published: Electronic Citation Status MEDLINE |
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| doi: |
10.1038/s41467-023-43115-3 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM364372745 |
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| 245 | 1 | 0 | |a Release of Histone H3K4-reading transcription factors from chromosomes in mitosis is independent of adjacent H3 phosphorylation |
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| 500 | |a published: Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2023. The Author(s). | ||
| 520 | |a Histone modifications influence the recruitment of reader proteins to chromosomes to regulate events including transcription and cell division. The idea of a histone code, where combinations of modifications specify unique downstream functions, is widely accepted and can be demonstrated in vitro. For example, on synthetic peptides, phosphorylation of Histone H3 at threonine-3 (H3T3ph) prevents the binding of reader proteins that recognize trimethylation of the adjacent lysine-4 (H3K4me3), including the TAF3 component of TFIID. To study these combinatorial effects in cells, we analyzed the genome-wide distribution of H3T3ph and H3K4me2/3 during mitosis. We find that H3T3ph anti-correlates with adjacent H3K4me2/3 in cells, and that the PHD domain of TAF3 can bind H3K4me2/3 in isolated mitotic chromatin despite the presence of H3T3ph. Unlike in vitro, H3K4 readers are still displaced from chromosomes in mitosis in Haspin-depleted cells lacking H3T3ph. H3T3ph is therefore unlikely to be responsible for transcriptional downregulation during cell division | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 7 | |a Histones |2 NLM | |
| 650 | 7 | |a Transcription Factors |2 NLM | |
| 700 | 1 | |a Heer, Maninder |e verfasserin |4 aut | |
| 700 | 1 | |a Levasseur, Mark D |e verfasserin |4 aut | |
| 700 | 1 | |a Cartwright, Tyrell N |e verfasserin |4 aut | |
| 700 | 1 | |a Weston, Bethany |e verfasserin |4 aut | |
| 700 | 1 | |a Mitchell, Jennifer L |e verfasserin |4 aut | |
| 700 | 1 | |a Coxhead, Jonathan M |e verfasserin |4 aut | |
| 700 | 1 | |a Gaughan, Luke |e verfasserin |4 aut | |
| 700 | 1 | |a Prendergast, Lisa |e verfasserin |4 aut | |
| 700 | 1 | |a Rico, Daniel |e verfasserin |4 aut | |
| 700 | 1 | |a Higgins, Jonathan M G |e verfasserin |4 aut | |
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