Underrepresentation of activating KIR gene expression in single-cell RNA-seq data is due to KIR gene misassignment
© 2023 The Authors. European Journal of Immunology published by Wiley-VCH GmbH..
Standard single-cell RNA-sequencing alignment pipelines exhibit a propensity for misassigning killer immunoglobulin-like receptor (KIR) transcripts, thereby giving rise to inaccuracies in quantifying KIR expression. Alves et al. elucidated that these default workflows frequently misclassify activating KIR transcripts as inhibitory KIR expression, resulting in a skewed representation of the KIR repertoire.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:54 |
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| Enthalten in: |
European journal of immunology - 54(2024), 1 vom: 01. Jan., Seite e2350590 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Alves, Eric [VerfasserIn] |
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| Links: |
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| Themen: |
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| Anmerkungen: |
Date Completed 30.01.2024 Date Revised 30.04.2024 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1002/eji.202350590 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 520 | |a Standard single-cell RNA-sequencing alignment pipelines exhibit a propensity for misassigning killer immunoglobulin-like receptor (KIR) transcripts, thereby giving rise to inaccuracies in quantifying KIR expression. Alves et al. elucidated that these default workflows frequently misclassify activating KIR transcripts as inhibitory KIR expression, resulting in a skewed representation of the KIR repertoire | ||
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| 700 | 1 | |a Gaudieri, Silvana |e verfasserin |4 aut | |
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