Inhibition of FSP1 impairs early embryo developmental competence in pigs
Copyright © 2023 Elsevier Inc. All rights reserved..
Ferroptosis suppressor protein 1 (FSP1) is a glutathione-independent ferroptosis inhibitory factor. FSP1 has been found to play a crucial role in the regulation of mitochondrial function and ferroptosis. However, its function in porcine early embryonic development remains unknown. In the present research, we found that FSP1 was expressed at different stages during porcine early embryo development. Compared with the control condition, inhibition of FSP1 reduced the cleavage rate at 24 h and 48 h and the blastocyst rate at 144 h. In addition, inhibiting FSP1 reduced the blastocyst diameter, total cell number, and proliferation capacity. Further analysis showed that inhibition of FSP1 significantly increased the levels of ferrous ions (Fe2+) and MDA but not GPX4. We also found that inhibition of FSP1 significantly decreased mitochondrial membrane potential and ATP levels, which in turn caused excessive accumulation of ROS and decreased the levels of GSH and the activity of the intracellular antioxidant enzymes SOD and CAT in embryos. In conclusion, FSP1, an important regulator, participates in regulating the development and quality of porcine early embryos. Inhibition of FSP1 impairs blastocyst formation, induces glutathione-independent ferroptosis, and further leads to oxidative stress due to mitochondrial dysfunction, ultimately affecting the developmental competence and impairing the quality of porcine early embryos.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2024 2023 |
|---|---|
| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:214 |
|---|---|
| Enthalten in: |
Theriogenology - 214(2023) vom: 15. Jan., Seite 257-265 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Wang, Yan-Qiu [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
Antioxidants |
|---|
| Anmerkungen: |
Date Completed 29.11.2023 Date Revised 29.11.2023 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1016/j.theriogenology.2023.10.025 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM364361441 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM364361441 | ||
| 003 | DE-627 | ||
| 005 | 20231226095255.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231226s2024 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.theriogenology.2023.10.025 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1214.xml |
| 035 | |a (DE-627)NLM364361441 | ||
| 035 | |a (NLM)37944430 | ||
| 035 | |a (PII)S0093-691X(23)00427-2 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Wang, Yan-Qiu |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Inhibition of FSP1 impairs early embryo developmental competence in pigs |
| 264 | 1 | |c 2024 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 29.11.2023 | ||
| 500 | |a Date Revised 29.11.2023 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2023 Elsevier Inc. All rights reserved. | ||
| 520 | |a Ferroptosis suppressor protein 1 (FSP1) is a glutathione-independent ferroptosis inhibitory factor. FSP1 has been found to play a crucial role in the regulation of mitochondrial function and ferroptosis. However, its function in porcine early embryonic development remains unknown. In the present research, we found that FSP1 was expressed at different stages during porcine early embryo development. Compared with the control condition, inhibition of FSP1 reduced the cleavage rate at 24 h and 48 h and the blastocyst rate at 144 h. In addition, inhibiting FSP1 reduced the blastocyst diameter, total cell number, and proliferation capacity. Further analysis showed that inhibition of FSP1 significantly increased the levels of ferrous ions (Fe2+) and MDA but not GPX4. We also found that inhibition of FSP1 significantly decreased mitochondrial membrane potential and ATP levels, which in turn caused excessive accumulation of ROS and decreased the levels of GSH and the activity of the intracellular antioxidant enzymes SOD and CAT in embryos. In conclusion, FSP1, an important regulator, participates in regulating the development and quality of porcine early embryos. Inhibition of FSP1 impairs blastocyst formation, induces glutathione-independent ferroptosis, and further leads to oxidative stress due to mitochondrial dysfunction, ultimately affecting the developmental competence and impairing the quality of porcine early embryos | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Early embryo development | |
| 650 | 4 | |a FSP1 | |
| 650 | 4 | |a Mitochondrial Dysfunction | |
| 650 | 4 | |a Oxidative stress | |
| 650 | 4 | |a Porcine | |
| 650 | 7 | |a Antioxidants |2 NLM | |
| 650 | 7 | |a Glutathione |2 NLM | |
| 650 | 7 | |a GAN16C9B8O |2 NLM | |
| 700 | 1 | |a Qu, He-Xuan |e verfasserin |4 aut | |
| 700 | 1 | |a Dong, Yan-Wei |e verfasserin |4 aut | |
| 700 | 1 | |a Qi, Jia-Jia |e verfasserin |4 aut | |
| 700 | 1 | |a Wei, Hua-Kai |e verfasserin |4 aut | |
| 700 | 1 | |a Sun, Hao |e verfasserin |4 aut | |
| 700 | 1 | |a Jiang, Hao |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Jia-Bao |e verfasserin |4 aut | |
| 700 | 1 | |a Sun, Bo-Xing |e verfasserin |4 aut | |
| 700 | 1 | |a Liang, Shuang |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Theriogenology |d 1974 |g 214(2023) vom: 15. Jan., Seite 257-265 |w (DE-627)NLM00008154X |x 1879-3231 |7 nnns |
| 773 | 1 | 8 | |g volume:214 |g year:2023 |g day:15 |g month:01 |g pages:257-265 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1016/j.theriogenology.2023.10.025 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 214 |j 2023 |b 15 |c 01 |h 257-265 | ||