Excited-State Dynamics of a Photoacid : A Potential Probe for Recognizing Transition from Lamellar to Nonlamellar Inverted Structures of Liposome based Nanocarriers
© 2023 Wiley-VCH GmbH..
Liposomes of a cationic lipid dioctadecyldimethylammonium bromide (DODAB) are efficient nanocarriers of nucleic acids. Incorporation of a neutral lipid monoolein (MO) in excess (xMO >0.5) changes the lamellar organization of DODAB liposomes into non-lamellar inverted structures of DODAB/MO liposomes facilitating nucleic acid delivery to cells. Photoexcitation of 8-hydroxypyrene-1,3,6-trisulfonic acid (HPTS), a photoacid, initiates an excited state proton transfer (ESPT) reaction in its protonated form (ROH*) generating the deprotonated anionic form (RO- *). The fluorescence intensity ratio (IROH* /IRO-* ) of these two forms is governed by the ESPT dynamics, and increases with increasing MO content (xMO ) in the cationic liposomes of DODAB. Transition from lamellar organization of DODAB liposomes into non-lamellar inverted structures of DODAB/MO liposomes, due to incorporation of MO (xMO ~0.7), is manifested by a significant increase of ESPT time (τPT ) and the time constant of wobbling motion (τW ) of HPTS. Thus, the lamellar organizations of DODAB or DODAB-rich (xMO 0.2) liposomes and the non-lamellar organizations of MO-rich (xMO ~0.7) liposomes are recognized by significantly different excited state dynamics of the photoacid.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:25 |
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| Enthalten in: |
Chemphyschem : a European journal of chemical physics and physical chemistry - 25(2024), 2 vom: 15. Jan., Seite e202300635 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Ghosh, Gourab [VerfasserIn] |
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| Links: |
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| Themen: |
251IW5I21C |
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| Anmerkungen: |
Date Completed 17.01.2024 Date Revised 17.01.2024 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1002/cphc.202300635 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM36428076X |
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| 520 | |a Liposomes of a cationic lipid dioctadecyldimethylammonium bromide (DODAB) are efficient nanocarriers of nucleic acids. Incorporation of a neutral lipid monoolein (MO) in excess (xMO >0.5) changes the lamellar organization of DODAB liposomes into non-lamellar inverted structures of DODAB/MO liposomes facilitating nucleic acid delivery to cells. Photoexcitation of 8-hydroxypyrene-1,3,6-trisulfonic acid (HPTS), a photoacid, initiates an excited state proton transfer (ESPT) reaction in its protonated form (ROH*) generating the deprotonated anionic form (RO- *). The fluorescence intensity ratio (IROH* /IRO-* ) of these two forms is governed by the ESPT dynamics, and increases with increasing MO content (xMO ) in the cationic liposomes of DODAB. Transition from lamellar organization of DODAB liposomes into non-lamellar inverted structures of DODAB/MO liposomes, due to incorporation of MO (xMO ~0.7), is manifested by a significant increase of ESPT time (τPT ) and the time constant of wobbling motion (τW ) of HPTS. Thus, the lamellar organizations of DODAB or DODAB-rich (xMO 0.2) liposomes and the non-lamellar organizations of MO-rich (xMO ~0.7) liposomes are recognized by significantly different excited state dynamics of the photoacid | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Excited state proton transfer | |
| 650 | 4 | |a Lamellar organization | |
| 650 | 4 | |a Liposomes | |
| 650 | 4 | |a Nanocarrier | |
| 650 | 4 | |a Non-lamellar inverted structures | |
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| 700 | 1 | |a Roy, Debanjana Singha |e verfasserin |4 aut | |
| 700 | 1 | |a Ghosh, Ria |e verfasserin |4 aut | |
| 700 | 1 | |a Mukherjee, Dipanjan |e verfasserin |4 aut | |
| 700 | 1 | |a Biswas, Suman |e verfasserin |4 aut | |
| 700 | 1 | |a Roy, Lopamudra |e verfasserin |4 aut | |
| 700 | 1 | |a Chattopadhyay, Arpita |e verfasserin |4 aut | |
| 700 | 1 | |a Das, Ranjan |e verfasserin |4 aut | |
| 700 | 1 | |a Pal, Samir Kumar |e verfasserin |4 aut | |
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