Target ssDNA activates the NADase activity of prokaryotic SPARTA immune system
© 2023. The Author(s), under exclusive licence to Springer Nature America, Inc..
Argonaute proteins (Agos), which use small RNAs or DNAs as guides to recognize complementary nucleic acid targets, mediate RNA silencing in eukaryotes. In prokaryotes, Agos are involved in immunity: the short prokaryotic Ago/TIR-APAZ (SPARTA) immune system triggers cell death by degrading NAD+ in response to invading plasmids, but its molecular mechanisms remain unknown. Here we used cryo-electron microscopy to determine the structures of inactive monomeric and active tetrameric Crenotalea thermophila SPARTA complexes, revealing mechanisms underlying SPARTA assembly, RNA-guided recognition of target single-stranded DNA (ssDNA) and subsequent SPARTA tetramerization, as well as tetramerization-dependent NADase activation. The small RNA guides Ago to recognize its ssDNA target, inducing SPARTA tetramerization via both Ago- and TIR-mediated interactions and resulting in a two-stranded, parallel, head-to-tail TIR rearrangement primed for NAD+ hydrolysis. Our findings thus identify the molecular basis for target ssDNA-mediated SPARTA activation, which will facilitate the development of SPARTA-based biotechnological tools.
Medienart: |
E-Artikel |
---|
Erscheinungsjahr: |
2024 |
---|---|
Erschienen: |
2024 |
Enthalten in: |
Zur Gesamtaufnahme - volume:20 |
---|---|
Enthalten in: |
Nature chemical biology - 20(2024), 4 vom: 07. Apr., Seite 503-511 |
Sprache: |
Englisch |
---|
Beteiligte Personen: |
Zhang, Jun-Tao [VerfasserIn] |
---|
Links: |
---|
Themen: |
0U46U6E8UK |
---|
Anmerkungen: |
Date Completed 29.03.2024 Date Revised 03.04.2024 published: Print-Electronic Citation Status MEDLINE |
---|
doi: |
10.1038/s41589-023-01479-z |
---|
funding: |
|
---|---|
Förderinstitution / Projekttitel: |
|
PPN (Katalog-ID): |
NLM364243376 |
---|
LEADER | 01000caa a22002652 4500 | ||
---|---|---|---|
001 | NLM364243376 | ||
003 | DE-627 | ||
005 | 20240404234350.0 | ||
007 | cr uuu---uuuuu | ||
008 | 231226s2024 xx |||||o 00| ||eng c | ||
024 | 7 | |a 10.1038/s41589-023-01479-z |2 doi | |
028 | 5 | 2 | |a pubmed24n1364.xml |
035 | |a (DE-627)NLM364243376 | ||
035 | |a (NLM)37932528 | ||
040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
041 | |a eng | ||
100 | 1 | |a Zhang, Jun-Tao |e verfasserin |4 aut | |
245 | 1 | 0 | |a Target ssDNA activates the NADase activity of prokaryotic SPARTA immune system |
264 | 1 | |c 2024 | |
336 | |a Text |b txt |2 rdacontent | ||
337 | |a ƒaComputermedien |b c |2 rdamedia | ||
338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
500 | |a Date Completed 29.03.2024 | ||
500 | |a Date Revised 03.04.2024 | ||
500 | |a published: Print-Electronic | ||
500 | |a Citation Status MEDLINE | ||
520 | |a © 2023. The Author(s), under exclusive licence to Springer Nature America, Inc. | ||
520 | |a Argonaute proteins (Agos), which use small RNAs or DNAs as guides to recognize complementary nucleic acid targets, mediate RNA silencing in eukaryotes. In prokaryotes, Agos are involved in immunity: the short prokaryotic Ago/TIR-APAZ (SPARTA) immune system triggers cell death by degrading NAD+ in response to invading plasmids, but its molecular mechanisms remain unknown. Here we used cryo-electron microscopy to determine the structures of inactive monomeric and active tetrameric Crenotalea thermophila SPARTA complexes, revealing mechanisms underlying SPARTA assembly, RNA-guided recognition of target single-stranded DNA (ssDNA) and subsequent SPARTA tetramerization, as well as tetramerization-dependent NADase activation. The small RNA guides Ago to recognize its ssDNA target, inducing SPARTA tetramerization via both Ago- and TIR-mediated interactions and resulting in a two-stranded, parallel, head-to-tail TIR rearrangement primed for NAD+ hydrolysis. Our findings thus identify the molecular basis for target ssDNA-mediated SPARTA activation, which will facilitate the development of SPARTA-based biotechnological tools | ||
650 | 4 | |a Journal Article | |
650 | 7 | |a DNA, Single-Stranded |2 NLM | |
650 | 7 | |a NAD+ Nucleosidase |2 NLM | |
650 | 7 | |a EC 3.2.2.5 |2 NLM | |
650 | 7 | |a NAD |2 NLM | |
650 | 7 | |a 0U46U6E8UK |2 NLM | |
650 | 7 | |a RNA |2 NLM | |
650 | 7 | |a 63231-63-0 |2 NLM | |
700 | 1 | |a Wei, Xin-Yang |e verfasserin |4 aut | |
700 | 1 | |a Cui, Ning |e verfasserin |4 aut | |
700 | 1 | |a Tian, Ruilin |e verfasserin |4 aut | |
700 | 1 | |a Jia, Ning |e verfasserin |4 aut | |
773 | 0 | 8 | |i Enthalten in |t Nature chemical biology |d 2005 |g 20(2024), 4 vom: 07. Apr., Seite 503-511 |w (DE-627)NLM159707676 |x 1552-4469 |7 nnns |
773 | 1 | 8 | |g volume:20 |g year:2024 |g number:4 |g day:07 |g month:04 |g pages:503-511 |
856 | 4 | 0 | |u http://dx.doi.org/10.1038/s41589-023-01479-z |3 Volltext |
912 | |a GBV_USEFLAG_A | ||
912 | |a GBV_NLM | ||
951 | |a AR | ||
952 | |d 20 |j 2024 |e 4 |b 07 |c 04 |h 503-511 |