HCV treatment outcome depends on SNPs of IFNL3-Gene polymorphisms (rs12979860) and cirrhotic changes in liver parenchyma
© 2023 The Authors. Published by Elsevier Ltd..
The allelic discrimination of IFNL3-(rs12979860 C > T) polymorphism reveals ambiguous associations with the effectiveness of oral HCV treatment. Solitary intra peripheral-blood-mononuclear-cells (PBMCs) HCV-RNA antisense-strands are independently detected in naïve and experienced cases regardless of viremia or hepatic-parenchymal alterations. We examined the frequencies of IFNL3-genetic variants with chronic-HCV-induced liver changes during the sustained virologic response (SVR) by evaluating the PBMCs- HCV-PCR after oral antiviral therapy. Methods: Twelve weeks after finishing oral antiviral therapy, the effects of IFNL3-genetic variants were evaluated in three groups of patients: Group-I (n = 25) showed HCV-RNA negativity in both serum and PBMCs-, group II (n = 52) showed positivity of HCV-RNA in PBMCs, and group-III (n = 25) had positive HCV-RNA in serum. The genetic variants of the IFNL3-gene were estimated for all the enrolled cases and correlated with their hepatic image changes. Results: IFNL3-(rs12979860) genotyping in post-direct acting antivirals (DAAs) SVR and HCV-relapse revealed: a) high frequency of CC-genotype and C-allele in group I compared to group II (P < 0.005) and group III(P ≤ 0.05) when hepatic-parenchyma looks normal by ultrasound b) frequent CT-genotype and T-allele in group II compared with I(P < 0.01) and III(P < 0.05) when liver tissues are bright (early cirrhotic-changes) c) frequent TT-genotype and T-allele in group III relative to I (P < 0.05) and II (P ≤ 0.08) when liver-tissues appear coarse by ultrasound. Conclusion: Outcomes of HCV treatment depend on host IFNL3-gene polymorphism and hepatic-parenchymal changes. A high frequency of wild-CC-genotype and C-allele is observed in patients with normal hepatic parenchyma and that achieved SVR. Solitary relapse in PBMCs occurs on increasing CT-genotype frequency when liver tissues are bright. Serologic relapse is detected when TT-genotype and T-allele are dominant in association with the cirrhotic liver. Therefore, IFNL3-gene-SNP analysis as a genetic predictor in relation to ultra-sonographic hepatic-parenchymal changes could be valuable for selecting the patients with the highest priority for treatment.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:9 |
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| Enthalten in: |
Heliyon - 9(2023), 11 vom: 27. Nov., Seite e21194 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Abd Alla, Mohamed Darwish Ahmed [VerfasserIn] |
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| Links: |
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| Themen: |
DAAs |
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| Anmerkungen: |
Date Revised 07.11.2023 published: Electronic-eCollection Citation Status PubMed-not-MEDLINE |
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| doi: |
10.1016/j.heliyon.2023.e21194 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM364198907 |
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| 100 | 1 | |a Abd Alla, Mohamed Darwish Ahmed |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a HCV treatment outcome depends on SNPs of IFNL3-Gene polymorphisms (rs12979860) and cirrhotic changes in liver parenchyma |
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| 520 | |a © 2023 The Authors. Published by Elsevier Ltd. | ||
| 520 | |a The allelic discrimination of IFNL3-(rs12979860 C > T) polymorphism reveals ambiguous associations with the effectiveness of oral HCV treatment. Solitary intra peripheral-blood-mononuclear-cells (PBMCs) HCV-RNA antisense-strands are independently detected in naïve and experienced cases regardless of viremia or hepatic-parenchymal alterations. We examined the frequencies of IFNL3-genetic variants with chronic-HCV-induced liver changes during the sustained virologic response (SVR) by evaluating the PBMCs- HCV-PCR after oral antiviral therapy. Methods: Twelve weeks after finishing oral antiviral therapy, the effects of IFNL3-genetic variants were evaluated in three groups of patients: Group-I (n = 25) showed HCV-RNA negativity in both serum and PBMCs-, group II (n = 52) showed positivity of HCV-RNA in PBMCs, and group-III (n = 25) had positive HCV-RNA in serum. The genetic variants of the IFNL3-gene were estimated for all the enrolled cases and correlated with their hepatic image changes. Results: IFNL3-(rs12979860) genotyping in post-direct acting antivirals (DAAs) SVR and HCV-relapse revealed: a) high frequency of CC-genotype and C-allele in group I compared to group II (P < 0.005) and group III(P ≤ 0.05) when hepatic-parenchyma looks normal by ultrasound b) frequent CT-genotype and T-allele in group II compared with I(P < 0.01) and III(P < 0.05) when liver tissues are bright (early cirrhotic-changes) c) frequent TT-genotype and T-allele in group III relative to I (P < 0.05) and II (P ≤ 0.08) when liver-tissues appear coarse by ultrasound. Conclusion: Outcomes of HCV treatment depend on host IFNL3-gene polymorphism and hepatic-parenchymal changes. A high frequency of wild-CC-genotype and C-allele is observed in patients with normal hepatic parenchyma and that achieved SVR. Solitary relapse in PBMCs occurs on increasing CT-genotype frequency when liver tissues are bright. Serologic relapse is detected when TT-genotype and T-allele are dominant in association with the cirrhotic liver. Therefore, IFNL3-gene-SNP analysis as a genetic predictor in relation to ultra-sonographic hepatic-parenchymal changes could be valuable for selecting the patients with the highest priority for treatment | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a DAAs | |
| 650 | 4 | |a HCV-Hepatic-changes | |
| 650 | 4 | |a IFNL3-Gene-SNPs | |
| 650 | 4 | |a PBMCs-PCR | |
| 700 | 1 | |a Dawood, Reham M |e verfasserin |4 aut | |
| 700 | 1 | |a Rashed, Hassan Abd El-Hafeth |e verfasserin |4 aut | |
| 700 | 1 | |a El-Dessouky, Yasser Mohammed |e verfasserin |4 aut | |
| 700 | 1 | |a AbuFarrag, Galal AbdElhameed |e verfasserin |4 aut | |
| 700 | 1 | |a Ammar, Islam Abdelmawla Emran |e verfasserin |4 aut | |
| 700 | 1 | |a Mahmoud, Mohamed Mahmoud Abdel-Halim |e verfasserin |4 aut | |
| 700 | 1 | |a Salum, Ghada M |e verfasserin |4 aut | |
| 700 | 1 | |a Abu-Amer, Mohamed Zakaria |e verfasserin |4 aut | |
| 700 | 1 | |a Sekeen, Mohamed Abd Elrafaa Hassan |e verfasserin |4 aut | |
| 700 | 1 | |a Heggazy, Mohamed Mousa Ibraheem |e verfasserin |4 aut | |
| 700 | 1 | |a Altanbouly, Ahmed Mohamed Abdulhamid |e verfasserin |4 aut | |
| 700 | 1 | |a Abd El-Meguid, Mai |e verfasserin |4 aut | |
| 700 | 1 | |a El Awady, Mostafa K |e verfasserin |4 aut | |
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