Methylene blue as an adjuvant during cardiopulmonary resuscitation : an experimental study in rats
Copyright © 2023 Sociedade Brasileira de Anestesiologia. Published by Elsevier España S.L.U. All rights reserved..
INTRODUCTION: Methylene Blue (MB) has been shown to attenuate oxidative, inflammatory, myocardial, and neurological lesions during ischemia-reperfusion and has great potential during cardiac arrest. This study aimed to determine the effects of MB combined with epinephrine during cardiac arrest on myocardial and cerebral lesions.
METHOD: Thirty-eight male Wistar rats were randomly assigned to four groups: the sham group (SH, n = 5), and three groups subjected to cardiac arrest (n = 11/group) and treated with EPI 20 µg.kg-1 (EPI), EPI 20 µg.kg-1 + MB 2 mg.kg-1 (EPI + MB), or saline 0.9% 0.2 ml (CTL). Ventricular fibrillation was induced by direct electrical stimulation in the right ventricle for 3 minutes, and anoxia was maintained for 5 minutes. Cardiopulmonary Resuscitation (CPR) consisted of medications, ventilation, chest compressions, and defibrillation. After returning to spontaneous circulation, animals were observed for four hours. Blood gas, troponin, oxidative stress, histology, and TUNEL staining measurements were analyzed. Groups were compared using generalized estimating equations.
RESULTS: No differences in the Returning of Spontaneous Circulation (ROSC) rate were observed among the groups (EPI: 63%, EPI + MB: 45%, CTL: 40%, p = 0.672). The mean arterial pressure immediately after ROSC was higher in the EPI+MB group than in the CTRL group (CTL: 30.5 [5.8], EPI: 63 [25.5], EPI+MB: 123 [31] mmHg, p = 0.007). Serum troponin levels were high in the CTL group (CTL: 130.1 [333.8], EPI: 3.70 [36.0], EPI + MB: 43.7 [116.31] ng/mL, p < 0.05).
CONCLUSION: The coadministration of MB and epinephrine failed to yield enhancements in cardiac or brain lesions in a rodent model of cardiac arrest.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:74 |
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| Enthalten in: |
Brazilian journal of anesthesiology (Elsevier) - 74(2024), 2 vom: 06. März, Seite 744470 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Xavier, Marcelo Souza [VerfasserIn] |
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| Links: |
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| Themen: |
Cardiac arrest |
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| Anmerkungen: |
Date Completed 25.03.2024 Date Revised 28.03.2024 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.bjane.2023.10.003 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM364182113 |
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| 100 | 1 | |a Xavier, Marcelo Souza |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Methylene blue as an adjuvant during cardiopulmonary resuscitation |b an experimental study in rats |
| 264 | 1 | |c 2024 | |
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| 500 | |a Date Completed 25.03.2024 | ||
| 500 | |a Date Revised 28.03.2024 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2023 Sociedade Brasileira de Anestesiologia. Published by Elsevier España S.L.U. All rights reserved. | ||
| 520 | |a INTRODUCTION: Methylene Blue (MB) has been shown to attenuate oxidative, inflammatory, myocardial, and neurological lesions during ischemia-reperfusion and has great potential during cardiac arrest. This study aimed to determine the effects of MB combined with epinephrine during cardiac arrest on myocardial and cerebral lesions | ||
| 520 | |a METHOD: Thirty-eight male Wistar rats were randomly assigned to four groups: the sham group (SH, n = 5), and three groups subjected to cardiac arrest (n = 11/group) and treated with EPI 20 µg.kg-1 (EPI), EPI 20 µg.kg-1 + MB 2 mg.kg-1 (EPI + MB), or saline 0.9% 0.2 ml (CTL). Ventricular fibrillation was induced by direct electrical stimulation in the right ventricle for 3 minutes, and anoxia was maintained for 5 minutes. Cardiopulmonary Resuscitation (CPR) consisted of medications, ventilation, chest compressions, and defibrillation. After returning to spontaneous circulation, animals were observed for four hours. Blood gas, troponin, oxidative stress, histology, and TUNEL staining measurements were analyzed. Groups were compared using generalized estimating equations | ||
| 520 | |a RESULTS: No differences in the Returning of Spontaneous Circulation (ROSC) rate were observed among the groups (EPI: 63%, EPI + MB: 45%, CTL: 40%, p = 0.672). The mean arterial pressure immediately after ROSC was higher in the EPI+MB group than in the CTRL group (CTL: 30.5 [5.8], EPI: 63 [25.5], EPI+MB: 123 [31] mmHg, p = 0.007). Serum troponin levels were high in the CTL group (CTL: 130.1 [333.8], EPI: 3.70 [36.0], EPI + MB: 43.7 [116.31] ng/mL, p < 0.05) | ||
| 520 | |a CONCLUSION: The coadministration of MB and epinephrine failed to yield enhancements in cardiac or brain lesions in a rodent model of cardiac arrest | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Cardiac arrest | |
| 650 | 4 | |a Epinephrine | |
| 650 | 4 | |a Hypoxia-ischemia | |
| 650 | 4 | |a Injury | |
| 650 | 4 | |a Methylene blue | |
| 650 | 4 | |a Rats | |
| 650 | 4 | |a Reperfusion | |
| 650 | 7 | |a Methylene Blue |2 NLM | |
| 650 | 7 | |a T42P99266K |2 NLM | |
| 650 | 7 | |a Epinephrine |2 NLM | |
| 650 | 7 | |a YKH834O4BH |2 NLM | |
| 650 | 7 | |a Troponin |2 NLM | |
| 700 | 1 | |a Vane, Matheus F |e verfasserin |4 aut | |
| 700 | 1 | |a Vieira, Roberta F |e verfasserin |4 aut | |
| 700 | 1 | |a Oliveira, Cristiano C |e verfasserin |4 aut | |
| 700 | 1 | |a Maia, Debora R R |e verfasserin |4 aut | |
| 700 | 1 | |a de Castro, Leticia U C |e verfasserin |4 aut | |
| 700 | 1 | |a Carmona, Maria José Carvalho |e verfasserin |4 aut | |
| 700 | 1 | |a Costa Auler, José Otávio |c Jr |e verfasserin |4 aut | |
| 700 | 1 | |a Otsuki, Denise Aya |e verfasserin |4 aut | |
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