Long Non-Coding RNA AL928768.3 Promotes Rheumatoid Arthritis Fibroblast-Like Synoviocytes Proliferation, Invasion and Inflammation, While Inhibits Apoptosis Via Activating Lymphotoxin Beta Mediated NF-κB Signaling Pathway
© 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature..
Our previous study using RNA sequencing and reverse transcription quantitative polymerase chain reaction (RT-qPCR) validation identified a long non-coding RNA (lnc), lnc-AL928768.3, correlating with risk and disease activity of rheumatoid arthritis (RA), then the present study was conducted to further investigate the interaction of lnc-AL928768.3 with lymphotoxin beta (LTB) and their impact on proliferation, migration, invasion, and inflammation in RA-fibroblast-like synoviocytes (RA-FLS). Human RA-FLS was obtained and transfected with lnc-AL928768.3 overexpression, negative control overexpression, lnc-AL928768.3 short hairpin RNA (shRNA) and negative control shRNA plasmids. Then cell functions and inflammatory cytokine expressions were detected. Afterward, rescue experiments were conducted via transfecting lnc-AL928768.3 shRNA with or without LTB overexpression plasmids in RA-FLS. Lnc-AL928768.3 enhanced proliferation and invasion, inhibited apoptosis, while had little impact on migration in RA-FLS. In addition, lnc-AL928768.3 positively modulated interleukin-1β (IL-1β), IL-6 and IL-8 expressions in RA-FLS supernatant; moreover, it also positively regulated LTB mRNA expression, LTB protein expression, p-NF-κB protein expression, and p-IKB-α protein expression in RA-FLS. Furthermore, following experiment showed that lnc-AL928768.3 positively regulated LTB expression while LTB did not impact on lnc-AL928768.3 expression in RA-FLS. Furthermore, in rescue experiments, LTB overexpression curtailed the effect of lnc-AL928768.3 knock-down on regulating proliferation, invasion, apoptosis and inflammatory cytokine expressions in RA-FLS. Lnc-AL928768.3 promotes proliferation, invasion, and inflammation while inhibits apoptosis of RA-FLS via activating LTB mediated NF-κB signaling.
Medienart: |
E-Artikel |
---|
Erscheinungsjahr: |
2024 |
---|---|
Erschienen: |
2024 |
Enthalten in: |
Zur Gesamtaufnahme - volume:47 |
---|---|
Enthalten in: |
Inflammation - 47(2024), 2 vom: 29. Apr., Seite 543-556 |
Sprache: |
Englisch |
---|
Beteiligte Personen: |
Sun, Li [VerfasserIn] |
---|
Links: |
---|
Themen: |
Cell function |
---|
Anmerkungen: |
Date Completed 06.05.2024 Date Revised 06.05.2024 published: Print-Electronic Citation Status MEDLINE |
---|
doi: |
10.1007/s10753-023-01927-x |
---|
funding: |
|
---|---|
Förderinstitution / Projekttitel: |
|
PPN (Katalog-ID): |
NLM364113960 |
---|
LEADER | 01000caa a22002652 4500 | ||
---|---|---|---|
001 | NLM364113960 | ||
003 | DE-627 | ||
005 | 20240506232130.0 | ||
007 | cr uuu---uuuuu | ||
008 | 231226s2024 xx |||||o 00| ||eng c | ||
024 | 7 | |a 10.1007/s10753-023-01927-x |2 doi | |
028 | 5 | 2 | |a pubmed24n1399.xml |
035 | |a (DE-627)NLM364113960 | ||
035 | |a (NLM)37919527 | ||
040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
041 | |a eng | ||
100 | 1 | |a Sun, Li |e verfasserin |4 aut | |
245 | 1 | 0 | |a Long Non-Coding RNA AL928768.3 Promotes Rheumatoid Arthritis Fibroblast-Like Synoviocytes Proliferation, Invasion and Inflammation, While Inhibits Apoptosis Via Activating Lymphotoxin Beta Mediated NF-κB Signaling Pathway |
264 | 1 | |c 2024 | |
336 | |a Text |b txt |2 rdacontent | ||
337 | |a ƒaComputermedien |b c |2 rdamedia | ||
338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
500 | |a Date Completed 06.05.2024 | ||
500 | |a Date Revised 06.05.2024 | ||
500 | |a published: Print-Electronic | ||
500 | |a Citation Status MEDLINE | ||
520 | |a © 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature. | ||
520 | |a Our previous study using RNA sequencing and reverse transcription quantitative polymerase chain reaction (RT-qPCR) validation identified a long non-coding RNA (lnc), lnc-AL928768.3, correlating with risk and disease activity of rheumatoid arthritis (RA), then the present study was conducted to further investigate the interaction of lnc-AL928768.3 with lymphotoxin beta (LTB) and their impact on proliferation, migration, invasion, and inflammation in RA-fibroblast-like synoviocytes (RA-FLS). Human RA-FLS was obtained and transfected with lnc-AL928768.3 overexpression, negative control overexpression, lnc-AL928768.3 short hairpin RNA (shRNA) and negative control shRNA plasmids. Then cell functions and inflammatory cytokine expressions were detected. Afterward, rescue experiments were conducted via transfecting lnc-AL928768.3 shRNA with or without LTB overexpression plasmids in RA-FLS. Lnc-AL928768.3 enhanced proliferation and invasion, inhibited apoptosis, while had little impact on migration in RA-FLS. In addition, lnc-AL928768.3 positively modulated interleukin-1β (IL-1β), IL-6 and IL-8 expressions in RA-FLS supernatant; moreover, it also positively regulated LTB mRNA expression, LTB protein expression, p-NF-κB protein expression, and p-IKB-α protein expression in RA-FLS. Furthermore, following experiment showed that lnc-AL928768.3 positively regulated LTB expression while LTB did not impact on lnc-AL928768.3 expression in RA-FLS. Furthermore, in rescue experiments, LTB overexpression curtailed the effect of lnc-AL928768.3 knock-down on regulating proliferation, invasion, apoptosis and inflammatory cytokine expressions in RA-FLS. Lnc-AL928768.3 promotes proliferation, invasion, and inflammation while inhibits apoptosis of RA-FLS via activating LTB mediated NF-κB signaling | ||
650 | 4 | |a Journal Article | |
650 | 4 | |a Cell function | |
650 | 4 | |a Inflammation | |
650 | 4 | |a Long non-coding RNA AL928768.3 | |
650 | 4 | |a Lymphotoxin beta | |
650 | 4 | |a Nuclear factor κB signaling pathway | |
650 | 4 | |a Rheumatoid arthritis | |
650 | 7 | |a RNA, Long Noncoding |2 NLM | |
650 | 7 | |a NF-kappa B |2 NLM | |
650 | 7 | |a Lymphotoxin-beta |2 NLM | |
700 | 1 | |a Hu, Lingzhen |e verfasserin |4 aut | |
700 | 1 | |a Chen, Peirong |e verfasserin |4 aut | |
700 | 1 | |a Li, Yongji |e verfasserin |4 aut | |
700 | 1 | |a Tu, Jianxin |e verfasserin |4 aut | |
700 | 1 | |a Chen, Jianghua |e verfasserin |4 aut | |
773 | 0 | 8 | |i Enthalten in |t Inflammation |d 1975 |g 47(2024), 2 vom: 29. Apr., Seite 543-556 |w (DE-627)NLM000293016 |x 1573-2576 |7 nnns |
773 | 1 | 8 | |g volume:47 |g year:2024 |g number:2 |g day:29 |g month:04 |g pages:543-556 |
856 | 4 | 0 | |u http://dx.doi.org/10.1007/s10753-023-01927-x |3 Volltext |
912 | |a GBV_USEFLAG_A | ||
912 | |a GBV_NLM | ||
951 | |a AR | ||
952 | |d 47 |j 2024 |e 2 |b 29 |c 04 |h 543-556 |