Metabotropic Glutamate Receptor 2 Expression Is Chronically Elevated in Male Rats With Post-Traumatic Stress Disorder Related Behavioral Traits Following Repetitive Low-Level Blast Exposure
Many military veterans who experienced blast-related traumatic brain injuries in the conflicts in Iraq and Afghanistan currently suffer from chronic cognitive and mental health problems that include depression and post-traumatic stress disorder (PTSD). Male rats exposed to repetitive low-level blast develop cognitive and PTSD-related behavioral traits that are present for more than 1 year after exposure. We previously reported that a group II metabotropic receptor (mGluR2/3) antagonist reversed blast-induced behavioral traits. In this report, we explored mGluR2/3 expression following blast exposure in male rats. Western blotting revealed that mGluR2 protein (but not mGluR3) was increased in all brain regions studied (anterior cortex, hippocampus, and amygdala) at 43 or 52 weeks after blast exposure but not at 2 weeks or 6 weeks. mGluR2 RNA was elevated at 52 weeks while mGluR3 was not. Immunohistochemical staining revealed no changes in the principally presynaptic localization of mGluR2 by blast exposure. Administering the mGluR2/3 antagonist LY341495 after behavioral traits had emerged rapidly reversed blast-induced effects on novel object recognition and cued fear responses 10 months following blast exposure. These studies support alterations in mGluR2 receptors as a key pathophysiological event following blast exposure and provide further support for group II metabotropic receptors as therapeutic targets in the neurobehavioral effects that follow blast injury.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2024 |
|---|---|
| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:41 |
|---|---|
| Enthalten in: |
Journal of neurotrauma - 41(2024), 5-6 vom: 01. März, Seite 714-733 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
De Gasperi, Rita [VerfasserIn] |
|---|
| Links: |
|---|
| Anmerkungen: |
Date Completed 04.03.2024 Date Revised 07.04.2024 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1089/neu.2023.0252 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM364089989 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM364089989 | ||
| 003 | DE-627 | ||
| 005 | 20240407232241.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231226s2024 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1089/neu.2023.0252 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1368.xml |
| 035 | |a (DE-627)NLM364089989 | ||
| 035 | |a (NLM)37917117 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a De Gasperi, Rita |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Metabotropic Glutamate Receptor 2 Expression Is Chronically Elevated in Male Rats With Post-Traumatic Stress Disorder Related Behavioral Traits Following Repetitive Low-Level Blast Exposure |
| 264 | 1 | |c 2024 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 04.03.2024 | ||
| 500 | |a Date Revised 07.04.2024 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Many military veterans who experienced blast-related traumatic brain injuries in the conflicts in Iraq and Afghanistan currently suffer from chronic cognitive and mental health problems that include depression and post-traumatic stress disorder (PTSD). Male rats exposed to repetitive low-level blast develop cognitive and PTSD-related behavioral traits that are present for more than 1 year after exposure. We previously reported that a group II metabotropic receptor (mGluR2/3) antagonist reversed blast-induced behavioral traits. In this report, we explored mGluR2/3 expression following blast exposure in male rats. Western blotting revealed that mGluR2 protein (but not mGluR3) was increased in all brain regions studied (anterior cortex, hippocampus, and amygdala) at 43 or 52 weeks after blast exposure but not at 2 weeks or 6 weeks. mGluR2 RNA was elevated at 52 weeks while mGluR3 was not. Immunohistochemical staining revealed no changes in the principally presynaptic localization of mGluR2 by blast exposure. Administering the mGluR2/3 antagonist LY341495 after behavioral traits had emerged rapidly reversed blast-induced effects on novel object recognition and cued fear responses 10 months following blast exposure. These studies support alterations in mGluR2 receptors as a key pathophysiological event following blast exposure and provide further support for group II metabotropic receptors as therapeutic targets in the neurobehavioral effects that follow blast injury | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Research Support, U.S. Gov't, Non-P.H.S. | |
| 650 | 4 | |a Research Support, N.I.H., Extramural | |
| 650 | 4 | |a blast | |
| 650 | 4 | |a group II metabotropic glutamate receptors | |
| 650 | 4 | |a male rats | |
| 650 | 4 | |a metabotropic glutamate receptor 2 | |
| 650 | 4 | |a military veterans | |
| 650 | 4 | |a traumatic brain injury | |
| 650 | 7 | |a metabotropic glutamate receptor 2 |2 NLM | |
| 650 | 7 | |a Receptors, Metabotropic Glutamate |2 NLM | |
| 700 | 1 | |a Gama Sosa, Miguel A |e verfasserin |4 aut | |
| 700 | 1 | |a Perez Garcia, Georgina |e verfasserin |4 aut | |
| 700 | 1 | |a Perez, Gissel M |e verfasserin |4 aut | |
| 700 | 1 | |a Pryor, Dylan |e verfasserin |4 aut | |
| 700 | 1 | |a Morrison, Chenel L-A |e verfasserin |4 aut | |
| 700 | 1 | |a Lind, Rachel |e verfasserin |4 aut | |
| 700 | 1 | |a Abutarboush, Rania |e verfasserin |4 aut | |
| 700 | 1 | |a Kawoos, Usmah |e verfasserin |4 aut | |
| 700 | 1 | |a Statz, Jonathan K |e verfasserin |4 aut | |
| 700 | 1 | |a Patterson, Jacob |e verfasserin |4 aut | |
| 700 | 1 | |a Hof, Patrick R |e verfasserin |4 aut | |
| 700 | 1 | |a Zhu, Carolyn W |e verfasserin |4 aut | |
| 700 | 1 | |a Ahlers, Stephen T |e verfasserin |4 aut | |
| 700 | 1 | |a Cook, David G |e verfasserin |4 aut | |
| 700 | 1 | |a Elder, Gregory A |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Journal of neurotrauma |d 1996 |g 41(2024), 5-6 vom: 01. März, Seite 714-733 |w (DE-627)NLM01272498X |x 1557-9042 |7 nnns |
| 773 | 1 | 8 | |g volume:41 |g year:2024 |g number:5-6 |g day:01 |g month:03 |g pages:714-733 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1089/neu.2023.0252 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 41 |j 2024 |e 5-6 |b 01 |c 03 |h 714-733 | ||