A marine cryptochrome with an inverse photo-oligomerization mechanism
© 2023. The Author(s)..
Cryptochromes (CRYs) are a structurally conserved but functionally diverse family of proteins that can confer unique sensory properties to organisms. In the marine bristle worm Platynereis dumerilii, its light receptive cryptochrome L-CRY (PdLCry) allows the animal to discriminate between sunlight and moonlight, an important requirement for synchronizing its lunar cycle-dependent mass spawning. Using cryo-electron microscopy, we show that in the dark, PdLCry adopts a dimer arrangement observed neither in plant nor insect CRYs. Intense illumination disassembles the dimer into monomers. Structural and functional data suggest a mechanistic coupling between the light-sensing flavin adenine dinucleotide chromophore, the dimer interface, and the C-terminal tail helix, with a likely involvement of the phosphate binding loop. Taken together, our work establishes PdLCry as a CRY protein with inverse photo-oligomerization with respect to plant CRYs, and provides molecular insights into how this protein might help discriminating the different light intensities associated with sunlight and moonlight.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:14 |
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| Enthalten in: |
Nature communications - 14(2023), 1 vom: 30. Okt., Seite 6918 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Vu, Hong Ha [VerfasserIn] |
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| Links: |
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| Themen: |
Cryptochromes |
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| Anmerkungen: |
Date Completed 01.11.2023 Date Revised 18.11.2023 published: Electronic Citation Status MEDLINE |
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| doi: |
10.1038/s41467-023-42708-2 |
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| Förderinstitution / Projekttitel: |
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NLM36395788X |
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| 245 | 1 | 2 | |a A marine cryptochrome with an inverse photo-oligomerization mechanism |
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| 500 | |a Date Revised 18.11.2023 | ||
| 500 | |a published: Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2023. The Author(s). | ||
| 520 | |a Cryptochromes (CRYs) are a structurally conserved but functionally diverse family of proteins that can confer unique sensory properties to organisms. In the marine bristle worm Platynereis dumerilii, its light receptive cryptochrome L-CRY (PdLCry) allows the animal to discriminate between sunlight and moonlight, an important requirement for synchronizing its lunar cycle-dependent mass spawning. Using cryo-electron microscopy, we show that in the dark, PdLCry adopts a dimer arrangement observed neither in plant nor insect CRYs. Intense illumination disassembles the dimer into monomers. Structural and functional data suggest a mechanistic coupling between the light-sensing flavin adenine dinucleotide chromophore, the dimer interface, and the C-terminal tail helix, with a likely involvement of the phosphate binding loop. Taken together, our work establishes PdLCry as a CRY protein with inverse photo-oligomerization with respect to plant CRYs, and provides molecular insights into how this protein might help discriminating the different light intensities associated with sunlight and moonlight | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 7 | |a Cryptochromes |2 NLM | |
| 700 | 1 | |a Behrmann, Heide |e verfasserin |4 aut | |
| 700 | 1 | |a Hanić, Maja |e verfasserin |4 aut | |
| 700 | 1 | |a Jeyasankar, Gayathri |e verfasserin |4 aut | |
| 700 | 1 | |a Krishnan, Shruthi |e verfasserin |4 aut | |
| 700 | 1 | |a Dannecker, Dennis |e verfasserin |4 aut | |
| 700 | 1 | |a Hammer, Constantin |e verfasserin |4 aut | |
| 700 | 1 | |a Gunkel, Monika |e verfasserin |4 aut | |
| 700 | 1 | |a Solov'yov, Ilia A |e verfasserin |4 aut | |
| 700 | 1 | |a Wolf, Eva |e verfasserin |4 aut | |
| 700 | 1 | |a Behrmann, Elmar |e verfasserin |4 aut | |
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