The pleiotropic functions of Pri smORF peptides synchronize leg development regulators
Copyright: © 2023 Markus et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited..
The last decade witnesses the emergence of the abundant family of smORF peptides, encoded by small ORF (<100 codons), whose biological functions remain largely unexplored. Bioinformatic analyses here identify hundreds of putative smORF peptides expressed in Drosophila imaginal leg discs. Thanks to a functional screen in leg, we found smORF peptides involved in morphogenesis, including the pioneer smORF peptides Pri. Since we identified its target Ubr3 in the epidermis and pri was known to control leg development through poorly understood mechanisms, we investigated the role of Ubr3 in mediating pri function in leg. We found that pri plays several roles during leg development both in patterning and in cell survival. During larval stage, pri activates independently of Ubr3 tarsal transcriptional programs and Notch and EGFR signaling pathways, whereas at larval pupal transition, Pri peptides cooperate with Ubr3 to insure cell survival and leg morphogenesis. Our results highlight Ubr3 dependent and independent functions of Pri peptides and their pleiotropy. Moreover, we reveal that the smORF peptide family is a reservoir of overlooked developmental regulators, displaying distinct molecular functions and orchestrating leg development.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2023 |
|---|---|
| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:19 |
|---|---|
| Enthalten in: |
PLoS genetics - 19(2023), 10 vom: 30. Okt., Seite e1011004 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Markus, Damien [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
Drosophila Proteins |
|---|
| Anmerkungen: |
Date Completed 16.11.2023 Date Revised 16.11.2023 published: Electronic-eCollection Citation Status MEDLINE |
|---|
| doi: |
10.1371/journal.pgen.1011004 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM363951687 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM363951687 | ||
| 003 | DE-627 | ||
| 005 | 20231226094429.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231226s2023 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1371/journal.pgen.1011004 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1213.xml |
| 035 | |a (DE-627)NLM363951687 | ||
| 035 | |a (NLM)37903161 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Markus, Damien |e verfasserin |4 aut | |
| 245 | 1 | 4 | |a The pleiotropic functions of Pri smORF peptides synchronize leg development regulators |
| 264 | 1 | |c 2023 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 16.11.2023 | ||
| 500 | |a Date Revised 16.11.2023 | ||
| 500 | |a published: Electronic-eCollection | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright: © 2023 Markus et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | ||
| 520 | |a The last decade witnesses the emergence of the abundant family of smORF peptides, encoded by small ORF (<100 codons), whose biological functions remain largely unexplored. Bioinformatic analyses here identify hundreds of putative smORF peptides expressed in Drosophila imaginal leg discs. Thanks to a functional screen in leg, we found smORF peptides involved in morphogenesis, including the pioneer smORF peptides Pri. Since we identified its target Ubr3 in the epidermis and pri was known to control leg development through poorly understood mechanisms, we investigated the role of Ubr3 in mediating pri function in leg. We found that pri plays several roles during leg development both in patterning and in cell survival. During larval stage, pri activates independently of Ubr3 tarsal transcriptional programs and Notch and EGFR signaling pathways, whereas at larval pupal transition, Pri peptides cooperate with Ubr3 to insure cell survival and leg morphogenesis. Our results highlight Ubr3 dependent and independent functions of Pri peptides and their pleiotropy. Moreover, we reveal that the smORF peptide family is a reservoir of overlooked developmental regulators, displaying distinct molecular functions and orchestrating leg development | ||
| 650 | 4 | |a Journal Article | |
| 650 | 7 | |a Drosophila Proteins |2 NLM | |
| 650 | 7 | |a Peptides |2 NLM | |
| 650 | 7 | |a Ubiquitin-Protein Ligases |2 NLM | |
| 650 | 7 | |a EC 2.3.2.27 |2 NLM | |
| 650 | 7 | |a Ubr3 protein, Drosophila |2 NLM | |
| 650 | 7 | |a EC 6.3.2.- |2 NLM | |
| 650 | 7 | |a Tal protein, Drosophila |2 NLM | |
| 650 | 7 | |a EC 2.2.1.2 |2 NLM | |
| 700 | 1 | |a Pelletier, Aurore |e verfasserin |4 aut | |
| 700 | 1 | |a Boube, Muriel |e verfasserin |4 aut | |
| 700 | 1 | |a Port, Fillip |e verfasserin |4 aut | |
| 700 | 1 | |a Boutros, Michael |e verfasserin |4 aut | |
| 700 | 1 | |a Payre, François |e verfasserin |4 aut | |
| 700 | 1 | |a Obermayer, Benedikt |e verfasserin |4 aut | |
| 700 | 1 | |a Zanet, Jennifer |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t PLoS genetics |d 2005 |g 19(2023), 10 vom: 30. Okt., Seite e1011004 |w (DE-627)NLM15722659X |x 1553-7404 |7 nnns |
| 773 | 1 | 8 | |g volume:19 |g year:2023 |g number:10 |g day:30 |g month:10 |g pages:e1011004 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1371/journal.pgen.1011004 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 19 |j 2023 |e 10 |b 30 |c 10 |h e1011004 | ||