Xanthohumol ameliorates cardiac injury induced by sepsis in a mice model : role of toll-like receptor 4
©2023 JOURNAL of MEDICINE and LIFE..
Sepsis, a life-threatening condition arising from infection, often results in multi-organ failure, including cardiac dysfunction. This study investigated Xanthohumol, a natural compound, and its potential mechanism of action to enhance heart function following sepsis. A total of twenty-four adult male Swiss albino mice were allocated randomly to one of four equal groups (n=6): sham, CLP, vehicle Xanthohumol the same amount of DMSO injected IP 10 minutes before the CLP, and Xanthohumol group (0.4 mg/kg of Xanthohumol administered IP before the CLP process). Toll-like receptor 4, pro-inflammatory mediators, anti-inflammatory markers, oxidative stress indicators, apoptosis markers, and serum cardiac damage biomarkers were measured in the cardiac tissue using ELISA. Data with normal distribution were analyzed using t-test and ANOVA tests (p<0.05). In comparison to the sham group, the sepsis group had significantly higher levels of TLR-4, IL-6, TNF-α, MIF, F2-isoprostane, caspase-3, cTn-I, and CK-MB, while the pre-treated group with Xanthohumol had significantly lower levels (p<0.05) of these markers than the sepsis group. Bcl-2 showed no significant difference in Xanthohumol pre-treated group relative to the sepsis group, while IL-10 was significantly elevated. Xanthohumol dramatically reduced cardiac tissue injury (p<0.05) relative to the CLP group. By blocking the downstream signal transduction pathways of TLR-4 and NF-kB, Xanthohumol was shown to lessen cardiac damage in male mice during CLP-induced polymicrobial sepsis.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:16 |
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| Enthalten in: |
Journal of medicine and life - 16(2023), 7 vom: 07. Juli, Seite 1105-1110 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Hadi, Sarah Mohammed Hussain [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 31.10.2023 Date Revised 31.10.2023 published: Print Citation Status MEDLINE |
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| doi: |
10.25122/jml-2023-0016 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM363920773 |
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| 100 | 1 | |a Hadi, Sarah Mohammed Hussain |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Xanthohumol ameliorates cardiac injury induced by sepsis in a mice model |b role of toll-like receptor 4 |
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| 500 | |a Date Completed 31.10.2023 | ||
| 500 | |a Date Revised 31.10.2023 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a ©2023 JOURNAL of MEDICINE and LIFE. | ||
| 520 | |a Sepsis, a life-threatening condition arising from infection, often results in multi-organ failure, including cardiac dysfunction. This study investigated Xanthohumol, a natural compound, and its potential mechanism of action to enhance heart function following sepsis. A total of twenty-four adult male Swiss albino mice were allocated randomly to one of four equal groups (n=6): sham, CLP, vehicle Xanthohumol the same amount of DMSO injected IP 10 minutes before the CLP, and Xanthohumol group (0.4 mg/kg of Xanthohumol administered IP before the CLP process). Toll-like receptor 4, pro-inflammatory mediators, anti-inflammatory markers, oxidative stress indicators, apoptosis markers, and serum cardiac damage biomarkers were measured in the cardiac tissue using ELISA. Data with normal distribution were analyzed using t-test and ANOVA tests (p<0.05). In comparison to the sham group, the sepsis group had significantly higher levels of TLR-4, IL-6, TNF-α, MIF, F2-isoprostane, caspase-3, cTn-I, and CK-MB, while the pre-treated group with Xanthohumol had significantly lower levels (p<0.05) of these markers than the sepsis group. Bcl-2 showed no significant difference in Xanthohumol pre-treated group relative to the sepsis group, while IL-10 was significantly elevated. Xanthohumol dramatically reduced cardiac tissue injury (p<0.05) relative to the CLP group. By blocking the downstream signal transduction pathways of TLR-4 and NF-kB, Xanthohumol was shown to lessen cardiac damage in male mice during CLP-induced polymicrobial sepsis | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a ANOVA: Analysis Of Variance | |
| 650 | 4 | |a Bcl-2: B-cell lymphoma 2 | |
| 650 | 4 | |a CK-MB: creatinine kinase MB | |
| 650 | 4 | |a CLP | |
| 650 | 4 | |a CLP: Cecal Ligation And Puncture | |
| 650 | 4 | |a C°: Celsius Degree | |
| 650 | 4 | |a DMSO: Dimethyl Sulfoxide | |
| 650 | 4 | |a ELISA: enzyme-linked immunosorbent assay | |
| 650 | 4 | |a ERK: Extracellular signal-regulated kinase | |
| 650 | 4 | |a IL-10: Interleukin 10 | |
| 650 | 4 | |a IL-6: Interleukin 6 | |
| 650 | 4 | |a IP: intraperitoneally | |
| 650 | 4 | |a JNK: c-Jun N-terminal kinase | |
| 650 | 4 | |a KEAP-1: Kelch-like ECH-associated protein 1 | |
| 650 | 4 | |a LPS: lipopolysaccharide | |
| 650 | 4 | |a MAPK: Mitogen-Activated Protein Kinase | |
| 650 | 4 | |a MD-2: Myeloid differentiation factor-2 | |
| 650 | 4 | |a MIF: Macrophage migration inhibitory | |
| 650 | 4 | |a NF-κB: Nuclear factor kappa-light-chain-enhancer of activated B cells | |
| 650 | 4 | |a Nrf2: nuclear factor erythroid 2-related factor 2 | |
| 650 | 4 | |a ROS: reactive oxygen species | |
| 650 | 4 | |a TLR- 4/NF- kB signaling pathways | |
| 650 | 4 | |a TLR4: Toll-like receptor 4 | |
| 650 | 4 | |a TNF- α: Tumor necrosis factor alpha | |
| 650 | 4 | |a XN: Xanthohumol | |
| 650 | 4 | |a Xanthohumol | |
| 650 | 4 | |a cTn-I: cardiac troponin I | |
| 650 | 4 | |a sepsis | |
| 650 | 7 | |a Toll-Like Receptor 4 |2 NLM | |
| 650 | 7 | |a xanthohumol |2 NLM | |
| 650 | 7 | |a T4467YT1NT |2 NLM | |
| 650 | 7 | |a NF-kappa B |2 NLM | |
| 700 | 1 | |a Majeed, Sahar |e verfasserin |4 aut | |
| 700 | 1 | |a Ghafil, Fadhaa Abdulameer |e verfasserin |4 aut | |
| 700 | 1 | |a Altoraihi, Kaswer |e verfasserin |4 aut | |
| 700 | 1 | |a Hadi, Najah Rayish |e verfasserin |4 aut | |
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