Autoantibody Profiling and Anti-Kinesin Reactivity in ANCA-Associated Vasculitis
ANCA-associated vasculitides (AAV) are rare autoimmune diseases causing inflammation and damage to small blood vessels. New autoantibody biomarkers are needed to improve the diagnosis and treatment of AAV patients. In this study, we aimed to profile the autoantibody repertoire of AAV patients using in-house developed antigen arrays to identify previously unreported antibodies linked to the disease per se, clinical subgroups, or clinical activity. A total of 1743 protein fragments representing 1561 unique proteins were screened in 229 serum samples collected from 137 AAV patients at presentation, remission, and relapse. Additionally, serum samples from healthy individuals and patients with other type of vasculitis and autoimmune-inflammatory conditions were included to evaluate the specificity of the autoantibodies identified in AAV. Autoreactivity against members of the kinesin protein family were identified in AAV patients, healthy volunteers, and disease controls. Anti-KIF4A antibodies were significantly more prevalent in AAV. We also observed possible associations between anti-kinesin antibodies and clinically relevant features within AAV patients. Further verification studies will be needed to confirm these findings.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2023 |
|---|---|
| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:24 |
|---|---|
| Enthalten in: |
International journal of molecular sciences - 24(2023), 20 vom: 19. Okt. |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Mescia, Federica [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
ANCA-associated vasculitis |
|---|
| Anmerkungen: |
Date Completed 30.10.2023 Date Revised 30.10.2023 published: Electronic Citation Status MEDLINE |
|---|
| doi: |
10.3390/ijms242015341 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM363870385 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM363870385 | ||
| 003 | DE-627 | ||
| 005 | 20231226094246.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231226s2023 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.3390/ijms242015341 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1212.xml |
| 035 | |a (DE-627)NLM363870385 | ||
| 035 | |a (NLM)37895021 | ||
| 035 | |a (PII)15341 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Mescia, Federica |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Autoantibody Profiling and Anti-Kinesin Reactivity in ANCA-Associated Vasculitis |
| 264 | 1 | |c 2023 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 30.10.2023 | ||
| 500 | |a Date Revised 30.10.2023 | ||
| 500 | |a published: Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a ANCA-associated vasculitides (AAV) are rare autoimmune diseases causing inflammation and damage to small blood vessels. New autoantibody biomarkers are needed to improve the diagnosis and treatment of AAV patients. In this study, we aimed to profile the autoantibody repertoire of AAV patients using in-house developed antigen arrays to identify previously unreported antibodies linked to the disease per se, clinical subgroups, or clinical activity. A total of 1743 protein fragments representing 1561 unique proteins were screened in 229 serum samples collected from 137 AAV patients at presentation, remission, and relapse. Additionally, serum samples from healthy individuals and patients with other type of vasculitis and autoimmune-inflammatory conditions were included to evaluate the specificity of the autoantibodies identified in AAV. Autoreactivity against members of the kinesin protein family were identified in AAV patients, healthy volunteers, and disease controls. Anti-KIF4A antibodies were significantly more prevalent in AAV. We also observed possible associations between anti-kinesin antibodies and clinically relevant features within AAV patients. Further verification studies will be needed to confirm these findings | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a ANCA-associated vasculitis | |
| 650 | 4 | |a autoantibody profiling | |
| 650 | 4 | |a biomarkers | |
| 650 | 4 | |a kinesins | |
| 650 | 4 | |a protein array | |
| 650 | 7 | |a Autoantibodies |2 NLM | |
| 650 | 7 | |a Kinesins |2 NLM | |
| 650 | 7 | |a EC 3.6.4.4 |2 NLM | |
| 650 | 7 | |a Biomarkers |2 NLM | |
| 650 | 7 | |a Proteins |2 NLM | |
| 700 | 1 | |a Bayati, Shaghayegh |e verfasserin |4 aut | |
| 700 | 1 | |a Brouwer, Elisabeth |e verfasserin |4 aut | |
| 700 | 1 | |a Heeringa, Peter |e verfasserin |4 aut | |
| 700 | 1 | |a Toonen, Erik J M |e verfasserin |4 aut | |
| 700 | 1 | |a Beenes, Marijke |e verfasserin |4 aut | |
| 700 | 1 | |a Ball, Miriam J |e verfasserin |4 aut | |
| 700 | 1 | |a Rees, Andrew J |e verfasserin |4 aut | |
| 700 | 1 | |a Kain, Renate |e verfasserin |4 aut | |
| 700 | 1 | |a Lyons, Paul A |e verfasserin |4 aut | |
| 700 | 1 | |a Nilsson, Peter |e verfasserin |4 aut | |
| 700 | 1 | |a Pin, Elisa |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t International journal of molecular sciences |d 2008 |g 24(2023), 20 vom: 19. Okt. |w (DE-627)NLM185552161 |x 1422-0067 |7 nnns |
| 773 | 1 | 8 | |g volume:24 |g year:2023 |g number:20 |g day:19 |g month:10 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.3390/ijms242015341 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 24 |j 2023 |e 20 |b 19 |c 10 | ||