An Improved Method for Growing Primary Neurons on Electron Microscopy Grids Co-Cultured with Astrocytes
With the increasing popularity of cryo-electron tomography (cryo-ET) in recent years, the quest to establish a method for growing primary neurons directly on electron microscopy grids (EM grids) has been ongoing. Here we describe a straightforward way to establish a mature neuronal network on EM grids, which includes formation of synaptic contacts. These synapses were thin enough to allow for direct visualization of small filaments such as SNARE proteins tethering the synaptic vesicle (SV) to the active zone plasma membrane on a Titan Krios without prior focused ion-beam milling.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:24 |
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| Enthalten in: |
International journal of molecular sciences - 24(2023), 20 vom: 14. Okt. |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Kumar, Ishika [VerfasserIn] |
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| Links: |
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| Themen: |
Astrocytes |
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| Anmerkungen: |
Date Completed 30.10.2023 Date Revised 10.02.2024 published: Electronic Citation Status MEDLINE |
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| doi: |
10.3390/ijms242015191 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 520 | |a With the increasing popularity of cryo-electron tomography (cryo-ET) in recent years, the quest to establish a method for growing primary neurons directly on electron microscopy grids (EM grids) has been ongoing. Here we describe a straightforward way to establish a mature neuronal network on EM grids, which includes formation of synaptic contacts. These synapses were thin enough to allow for direct visualization of small filaments such as SNARE proteins tethering the synaptic vesicle (SV) to the active zone plasma membrane on a Titan Krios without prior focused ion-beam milling | ||
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| 700 | 1 | |a Sørensen, Jakob Balslev |e verfasserin |4 aut | |
| 700 | 1 | |a Radecke, Julika |e verfasserin |4 aut | |
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