Construction of L-type lectin displaying Saccharomyces cerevisiae for Vibrio parahaemolyticus agglutination
© 2023. The Author(s), under exclusive licence to Springer Nature Switzerland AG..
pYIP-LvLTLC1-Aga1P was confirmed through the use of analytical techniques including SDS-PAGE, dot blot, and fluorescent immunoassay with LvLTC1-specific antibody. Subsequently, the newly generated yeast strain was evaluated for its ability to agglutinate V. parahaemolyticus and A. hydrophila. The obtained results indicated that S. cerevisiae expressing LvLTLC1 protein on its surface had the ability to agglutinate both AHPND-causing V. parahaemolyticus and A. hydrophila. This newly generated yeast strain could be served as a feed supplement for controlling bacteria in general and AHPND in particular.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2023 |
|---|---|
| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - year:2023 |
|---|---|
| Enthalten in: |
International microbiology : the official journal of the Spanish Society for Microbiology - (2023) vom: 27. Okt. |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Nguyen, Phuong-Thao Thi [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
AHPND |
|---|
| Anmerkungen: |
Date Revised 27.10.2023 published: Print-Electronic Citation Status Publisher |
|---|
| doi: |
10.1007/s10123-023-00440-3 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM363814485 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM363814485 | ||
| 003 | DE-627 | ||
| 005 | 20231226094136.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231226s2023 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1007/s10123-023-00440-3 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1212.xml |
| 035 | |a (DE-627)NLM363814485 | ||
| 035 | |a (NLM)37889383 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Nguyen, Phuong-Thao Thi |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Construction of L-type lectin displaying Saccharomyces cerevisiae for Vibrio parahaemolyticus agglutination |
| 264 | 1 | |c 2023 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Revised 27.10.2023 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status Publisher | ||
| 520 | |a © 2023. The Author(s), under exclusive licence to Springer Nature Switzerland AG. | ||
| 520 | |a The utilization of Aga1P anchor protein in the display system for expressing heterologous proteins on the surface of Saccharomyces cerevisiae has been shown to be an ideal approach. This system has the ability to improve the expression of target proteins beyond the cell surface, resulting in increased activity and stability of the expression system. Recent studies have demonstrated that a new L-type lectin from Litopenaeus vannamei (LvLTLC1) has been found to possess the capability of agglutinating Vibrio parahaemolyticus, a pathogen responsible for causing acute hepatopancreatic necrosis disease (AHPND) in shrimp. In this study, LvLTLC1 protein was designed to be expressed on the surface of S. cerevisiae via Aga1P anchor. The expression of LvLTLC1 protein on the surface of S. cerevisiae::pYIP-LvLTLC1-Aga1P was confirmed through the use of analytical techniques including SDS-PAGE, dot blot, and fluorescent immunoassay with LvLTC1-specific antibody. Subsequently, the newly generated yeast strain was evaluated for its ability to agglutinate V. parahaemolyticus and A. hydrophila. The obtained results indicated that S. cerevisiae expressing LvLTLC1 protein on its surface had the ability to agglutinate both AHPND-causing V. parahaemolyticus and A. hydrophila. This newly generated yeast strain could be served as a feed supplement for controlling bacteria in general and AHPND in particular | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a AHPND | |
| 650 | 4 | |a L-type lectin | |
| 650 | 4 | |a LvLTLC1 | |
| 650 | 4 | |a Saccharomyces cerevisiae | |
| 650 | 4 | |a Vibrio parahaemolyticus | |
| 650 | 4 | |a Yeast surface display | |
| 700 | 1 | |a Dinh, Thuan-Thien |e verfasserin |4 aut | |
| 700 | 1 | |a Tran-Van, Hieu |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t International microbiology : the official journal of the Spanish Society for Microbiology |d 1998 |g (2023) vom: 27. Okt. |w (DE-627)NLM097544469 |x 1618-1905 |7 nnns |
| 773 | 1 | 8 | |g year:2023 |g day:27 |g month:10 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1007/s10123-023-00440-3 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |j 2023 |b 27 |c 10 | ||