LC-Orbitrap-HRMS Determination of Two Novel Plastochromanol Homologues
© 2023 The Authors. Molecular Nutrition & Food Research published by Wiley-VCH GmbH..
SCOPE: The antioxidant plastochromanol-8 (PC-8) is a tocochromanol which differs from γ-tocotrienol in having an unsaturated side chain of eight instead of three isoprene units. The recent isolation of PC-8 from flaxseed oil indicates the additional presence of lower shares of two previously unknown homologues, plastochromanol-7 (PC-7) and plastochromanol-9 (PC-9), which feature seven and nine isoprenoid units respectively on the γ-chromanol backbone. Here, a fast LC-Orbitrap-HRMS method is applied for the determination of PC-7 and PC-9 in seven plant oils and a plant extract.
METHODS AND RESULTS: The presence of PC-7, PC-8, and PC-9 is confirmed in all eight investigated samples by LC-Orbitrap-HRMS analysis after saponification. PC-8 amounts of ≈315-350 mg kg-1 in two flaxseed oils, ≈75 mg kg-1 in rapeseed oil, ≈38 mg kg-1 in camelina oil, ≈80-120 mg kg-1 in two mustard oils, ≈90 mg kg-1 in candle nut oil, and ≈900 mg kg-1 dry weight in Cecropia leaves are determined by quantification. Semi-quantification of PC-7 and PC-9 indicated the presence of ≈0.1-1% of PC-7 and PC-9 in varied relative ratios.
CONCLUSION: The novel plastochromanol homologues are of particular interest to researchers with focus on vitamin E and other tocochromanols because of their unexplored bioactivity.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:67 |
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| Enthalten in: |
Molecular nutrition & food research - 67(2023), 24 vom: 17. Dez., Seite e2300333 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Hammerschick, Tim [VerfasserIn] |
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| Links: |
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| Themen: |
Antioxidants |
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| Anmerkungen: |
Date Completed 16.12.2023 Date Revised 16.12.2023 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1002/mnfr.202300333 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 520 | |a © 2023 The Authors. Molecular Nutrition & Food Research published by Wiley-VCH GmbH. | ||
| 520 | |a SCOPE: The antioxidant plastochromanol-8 (PC-8) is a tocochromanol which differs from γ-tocotrienol in having an unsaturated side chain of eight instead of three isoprene units. The recent isolation of PC-8 from flaxseed oil indicates the additional presence of lower shares of two previously unknown homologues, plastochromanol-7 (PC-7) and plastochromanol-9 (PC-9), which feature seven and nine isoprenoid units respectively on the γ-chromanol backbone. Here, a fast LC-Orbitrap-HRMS method is applied for the determination of PC-7 and PC-9 in seven plant oils and a plant extract | ||
| 520 | |a METHODS AND RESULTS: The presence of PC-7, PC-8, and PC-9 is confirmed in all eight investigated samples by LC-Orbitrap-HRMS analysis after saponification. PC-8 amounts of ≈315-350 mg kg-1 in two flaxseed oils, ≈75 mg kg-1 in rapeseed oil, ≈38 mg kg-1 in camelina oil, ≈80-120 mg kg-1 in two mustard oils, ≈90 mg kg-1 in candle nut oil, and ≈900 mg kg-1 dry weight in Cecropia leaves are determined by quantification. Semi-quantification of PC-7 and PC-9 indicated the presence of ≈0.1-1% of PC-7 and PC-9 in varied relative ratios | ||
| 520 | |a CONCLUSION: The novel plastochromanol homologues are of particular interest to researchers with focus on vitamin E and other tocochromanols because of their unexplored bioactivity | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a plastochromanol-7 | |
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