Divergent Roles of KLF4 During Primordial Germ Cell Fate Induction from Human Embryonic Stem Cells
© 2023. The Author(s), under exclusive licence to Society for Reproductive Investigation..
As a core transcriptional factor regulating pluripotency, Krüppel-like factor 4 (KLF4) has gained much attention in the field of stem cells during the past decades. However, few research have focused on the function of KLF4 during human primordial germ cell (PGC) specification. Here, we induced human PGC-like cells (hPGCLCs) from human embryonic stem cells (hESCs) and the derived hPGCLCs upregulated PGC-related genes, like SOX17, BLIMP1, TFAP2C, NANOS3, and the naïve pluripotency gene KLF4. The KLF4-knockout hESCs formed typical multicellular colonies with clear borders, expressed pluripotency genes, such as NANOG, OCT4, and SOX2, and exhibited no differences in proliferation capacity compared with wild type hESCs. Notably, KLF4 deletion in hESCs did not influence the induction of PGCLCs in vitro. In contrast, overexpression of KLF4 during PGC induction process inhibited the efficiency of PGCLC formation from hESCs in vitro. Overexpression of KLF4 may regenerate the naïve ground state in hESCs and results in repression for PGC specification. Thus, KLF4 could be a downstream target of human PGC program and the upregulation of KLF4 is prepared for late stage of germline development.
Medienart: |
E-Artikel |
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Erscheinungsjahr: |
2024 |
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Erschienen: |
2024 |
Enthalten in: |
Zur Gesamtaufnahme - volume:31 |
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Enthalten in: |
Reproductive sciences (Thousand Oaks, Calif.) - 31(2024), 3 vom: 02. März, Seite 727-735 |
Sprache: |
Englisch |
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Beteiligte Personen: |
Fang, Fang [VerfasserIn] |
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Links: |
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Themen: |
Human embryonic stem cells (hESCs) |
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Anmerkungen: |
Date Completed 06.03.2024 Date Revised 12.03.2024 published: Print-Electronic Citation Status MEDLINE |
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doi: |
10.1007/s43032-023-01360-3 |
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funding: |
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Förderinstitution / Projekttitel: |
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PPN (Katalog-ID): |
NLM363768149 |
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520 | |a As a core transcriptional factor regulating pluripotency, Krüppel-like factor 4 (KLF4) has gained much attention in the field of stem cells during the past decades. However, few research have focused on the function of KLF4 during human primordial germ cell (PGC) specification. Here, we induced human PGC-like cells (hPGCLCs) from human embryonic stem cells (hESCs) and the derived hPGCLCs upregulated PGC-related genes, like SOX17, BLIMP1, TFAP2C, NANOS3, and the naïve pluripotency gene KLF4. The KLF4-knockout hESCs formed typical multicellular colonies with clear borders, expressed pluripotency genes, such as NANOG, OCT4, and SOX2, and exhibited no differences in proliferation capacity compared with wild type hESCs. Notably, KLF4 deletion in hESCs did not influence the induction of PGCLCs in vitro. In contrast, overexpression of KLF4 during PGC induction process inhibited the efficiency of PGCLC formation from hESCs in vitro. Overexpression of KLF4 may regenerate the naïve ground state in hESCs and results in repression for PGC specification. Thus, KLF4 could be a downstream target of human PGC program and the upregulation of KLF4 is prepared for late stage of germline development | ||
650 | 4 | |a Journal Article | |
650 | 4 | |a Human embryonic stem cells (hESCs) | |
650 | 4 | |a Krüppel-like factor 4 (KLF4) | |
650 | 4 | |a Naïve pluripotency | |
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700 | 1 | |a Lu, Shi |e verfasserin |4 aut | |
700 | 1 | |a Wang, Xiao |e verfasserin |4 aut | |
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