Optimization of Plasmodium vivax infection of colonized Amazonian Anopheles darlingi
© 2023. Springer Nature Limited..
Obtaining Plasmodium vivax sporozoites is essential for in vitro culture of liver stage parasites, not only to understand fundamental aspects of parasite biology, but also for drug and vaccine development. A major impediment to establish high-throughput in vitro P. vivax liver stage assays for drug development is obtaining sufficient numbers of sporozoites. To do so, female anopheline mosquitoes have to be fed on blood from P. vivax-infected patients through an artificial membrane-feeding system, which in turns requires a well-established Anopheles colony. In this study we established conditions to provide a robust supply of P. vivax sporozoites. Adding a combination of serum replacement and antibiotics to the membrane-feeding protocol was found to best improve sporozoite production. A simple centrifugation method appears to be a possible tool for rapidly obtaining purified sporozoites with a minimal loss of yield. However, this method needs to be better defined since sporozoite viability and hepatocyte infection were not evaluated.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:13 |
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| Enthalten in: |
Scientific reports - 13(2023), 1 vom: 24. Okt., Seite 18207 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Andrade, Alice O [VerfasserIn] |
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| Anmerkungen: |
Date Completed 27.10.2023 Date Revised 12.03.2024 published: Electronic Citation Status MEDLINE |
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| doi: |
10.1038/s41598-023-44556-y |
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| 520 | |a Obtaining Plasmodium vivax sporozoites is essential for in vitro culture of liver stage parasites, not only to understand fundamental aspects of parasite biology, but also for drug and vaccine development. A major impediment to establish high-throughput in vitro P. vivax liver stage assays for drug development is obtaining sufficient numbers of sporozoites. To do so, female anopheline mosquitoes have to be fed on blood from P. vivax-infected patients through an artificial membrane-feeding system, which in turns requires a well-established Anopheles colony. In this study we established conditions to provide a robust supply of P. vivax sporozoites. Adding a combination of serum replacement and antibiotics to the membrane-feeding protocol was found to best improve sporozoite production. A simple centrifugation method appears to be a possible tool for rapidly obtaining purified sporozoites with a minimal loss of yield. However, this method needs to be better defined since sporozoite viability and hepatocyte infection were not evaluated | ||
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| 700 | 1 | |a Pontual, José Daniel C |e verfasserin |4 aut | |
| 700 | 1 | |a Araújo, Cristiane S |e verfasserin |4 aut | |
| 700 | 1 | |a Lima, Analice S |e verfasserin |4 aut | |
| 700 | 1 | |a Martinez, Leandro N |e verfasserin |4 aut | |
| 700 | 1 | |a Ferreira, Amália S |e verfasserin |4 aut | |
| 700 | 1 | |a Aguiar, Anna Caroline C |e verfasserin |4 aut | |
| 700 | 1 | |a Teles, Carolina B G |e verfasserin |4 aut | |
| 700 | 1 | |a Guido, Rafael V C |e verfasserin |4 aut | |
| 700 | 1 | |a Santana, Rosa A |e verfasserin |4 aut | |
| 700 | 1 | |a Lopes, Stefanie C P |e verfasserin |4 aut | |
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| 700 | 1 | |a Campo, Brice |e verfasserin |4 aut | |
| 700 | 1 | |a Lacerda, Marcus Vinicius G |e verfasserin |4 aut | |
| 700 | 1 | |a Araújo, Maisa S |e verfasserin |4 aut | |
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