Preparation of arsenic(III) monoclonal antibodies and preliminary evaluation of a novel silver-coated gold nanorod SERS immunoassay strip construction
Heavy metal pollution has become a growing concern in industrial, agricultural, and manufacturing processes, posing a significant threat to human health. Among these heavy metals, arsenic (As) is highly toxic and shares similar chemical properties and environmental behavior with other heavy metals. As(III) is particularly toxic compared to other forms of arsenic. Therefore, it is essential to develop a real-time, rapid, and sensitive method for the determination of As(III). In this study, we employed a unique bifunctional chelator, 1-(4-isothiocyanobenzyl)-ethylenediamine N,N,N',N'-tetraacetic acid (ITCBE), to prepare a complete antigen. Through a series of tests including balb/c mouse immunization, cell fusion (mouse L2041 spleen cells with mouse myeloma cells SP2/0), and subcloning, we generated four monoclonal cell lines (1C1, 2C2, 3A9, and 4A11). These cell lines demonstrated high purity, high affinity, and IC50 values of less than 50 μg mL-1. Monoclonal antibody 4A11, which exhibited a strong Raman signal, was selected as the probe, and AuAg 200 was utilized as the surface-enhanced Raman scattering (SERS) substrate for the preliminary establishment of SERS immunochromatographic test strips. The sensitivity of the SERS immunochromatographic test strips, measured through Raman signal detection, showed a significant improvement compared to the SERS immunochromatographic test strips analyzed by colorimetry (LOD = 49.43 μg mL-1 and LDR = 5.32-81.31 μg mL-1). The SERS immunochromatographic test strips achieved a LOD of 7.62 μg mL-1 and an LDR of 12.66-71.84 μg mL-1. This study presents innovative methodologies for the rapid detection of As(III) using SERS immunochromatographic test strips.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2023 |
|---|---|
| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:15 |
|---|---|
| Enthalten in: |
Analytical methods : advancing methods and applications - 15(2023), 43 vom: 09. Nov., Seite 5823-5836 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Liang, Yi [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
3M4G523W1G |
|---|
| Anmerkungen: |
Date Completed 16.11.2023 Date Revised 16.11.2023 published: Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1039/d3ay01205k |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM363630716 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM363630716 | ||
| 003 | DE-627 | ||
| 005 | 20231226093749.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231226s2023 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1039/d3ay01205k |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1212.xml |
| 035 | |a (DE-627)NLM363630716 | ||
| 035 | |a (NLM)37870766 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Liang, Yi |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Preparation of arsenic(III) monoclonal antibodies and preliminary evaluation of a novel silver-coated gold nanorod SERS immunoassay strip construction |
| 264 | 1 | |c 2023 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 16.11.2023 | ||
| 500 | |a Date Revised 16.11.2023 | ||
| 500 | |a published: Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Heavy metal pollution has become a growing concern in industrial, agricultural, and manufacturing processes, posing a significant threat to human health. Among these heavy metals, arsenic (As) is highly toxic and shares similar chemical properties and environmental behavior with other heavy metals. As(III) is particularly toxic compared to other forms of arsenic. Therefore, it is essential to develop a real-time, rapid, and sensitive method for the determination of As(III). In this study, we employed a unique bifunctional chelator, 1-(4-isothiocyanobenzyl)-ethylenediamine N,N,N',N'-tetraacetic acid (ITCBE), to prepare a complete antigen. Through a series of tests including balb/c mouse immunization, cell fusion (mouse L2041 spleen cells with mouse myeloma cells SP2/0), and subcloning, we generated four monoclonal cell lines (1C1, 2C2, 3A9, and 4A11). These cell lines demonstrated high purity, high affinity, and IC50 values of less than 50 μg mL-1. Monoclonal antibody 4A11, which exhibited a strong Raman signal, was selected as the probe, and AuAg 200 was utilized as the surface-enhanced Raman scattering (SERS) substrate for the preliminary establishment of SERS immunochromatographic test strips. The sensitivity of the SERS immunochromatographic test strips, measured through Raman signal detection, showed a significant improvement compared to the SERS immunochromatographic test strips analyzed by colorimetry (LOD = 49.43 μg mL-1 and LDR = 5.32-81.31 μg mL-1). The SERS immunochromatographic test strips achieved a LOD of 7.62 μg mL-1 and an LDR of 12.66-71.84 μg mL-1. This study presents innovative methodologies for the rapid detection of As(III) using SERS immunochromatographic test strips | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 7 | |a Antibodies, Monoclonal |2 NLM | |
| 650 | 7 | |a Arsenic |2 NLM | |
| 650 | 7 | |a N712M78A8G |2 NLM | |
| 650 | 7 | |a Gold |2 NLM | |
| 650 | 7 | |a 7440-57-5 |2 NLM | |
| 650 | 7 | |a Silver |2 NLM | |
| 650 | 7 | |a 3M4G523W1G |2 NLM | |
| 700 | 1 | |a Li, Hao |e verfasserin |4 aut | |
| 700 | 1 | |a Xu, Naifeng |e verfasserin |4 aut | |
| 700 | 1 | |a Zhu, Jiangxiong |e verfasserin |4 aut | |
| 700 | 1 | |a Wu, Xiaobin |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Yuanfeng |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Analytical methods : advancing methods and applications |d 2009 |g 15(2023), 43 vom: 09. Nov., Seite 5823-5836 |w (DE-627)NLM202500322 |x 1759-9679 |7 nnns |
| 773 | 1 | 8 | |g volume:15 |g year:2023 |g number:43 |g day:09 |g month:11 |g pages:5823-5836 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1039/d3ay01205k |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 15 |j 2023 |e 43 |b 09 |c 11 |h 5823-5836 | ||