Multicenter interlaboratory study of routine systems for the susceptibility testing of temocillin using a challenge panel of multidrug-resistant strains
© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature..
Accurate susceptibility result of temocillin (TMO) is important for treating infections caused by multidrug-resistant Enterobacterales. This multicenter study aimed to investigate the performance of routine temocillin testing assays against Enterobacterales challenging strains. Forty-seven selected clinical isolates were blindly analyzed by 12 Belgian laboratories using VITEK® 2 (n = 5) and BD Phoenix™ (n = 3) automated systems, ETEST® gradient strip (n = 3), and disk (3 brands) diffusion method (DD; n = 6) for temocillin susceptibility using standardized methodology. Results were interpreted using EUCAST 2023 criteria and compared to the broth microdilution (BMD; Sensititre™ panel) method used as gold standard. Methods' reproducibility was assessed by testing 3 reference strains in triplicate. A total of 702 organism-drug results were obtained against 33 TMO-susceptible and 14 TMO-resistant isolates. Excluding Proteae species (P. mirabilis and M. morganii), the essential agreement rates were excellent (91.5-100%) for all MIC-based methods. The highest category agreement was achieved by ETEST® (97.5%) followed by VITEK® 2 (93.2%), disk diffusion (91.6%), and BD Phoenix™ (88.5%). BD Phoenix™ and paper disk diffusion overcalled resistance (11.5% and 6.8% of major discrepancies, respectively), while ROSCO tablets diffusion and VITEK® 2 generated higher very major discrepancies (7.1% and 4.2% respectively). Inter-assay reproducibility was unsatisfactory using recommended E. coli ATCC 25922 strain but was excellent with E. coli ATCC 35218 and K. pneumoniae ATCC 700603 strains. This interlaboratory study suggests that routine testing methods provide accurate and reproducible TMO categorization results except for Proteae species.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2023 |
|---|---|
| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:42 |
|---|---|
| Enthalten in: |
European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology - 42(2023), 12 vom: 01. Dez., Seite 1477-1483 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Deckers, Corentin [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
03QB156W6I |
|---|
| Anmerkungen: |
Date Completed 14.02.2024 Date Revised 14.02.2024 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1007/s10096-023-04681-y |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM363630198 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM363630198 | ||
| 003 | DE-627 | ||
| 005 | 20240214232851.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231226s2023 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1007/s10096-023-04681-y |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1292.xml |
| 035 | |a (DE-627)NLM363630198 | ||
| 035 | |a (NLM)37870713 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Deckers, Corentin |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Multicenter interlaboratory study of routine systems for the susceptibility testing of temocillin using a challenge panel of multidrug-resistant strains |
| 264 | 1 | |c 2023 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 14.02.2024 | ||
| 500 | |a Date Revised 14.02.2024 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature. | ||
| 520 | |a Accurate susceptibility result of temocillin (TMO) is important for treating infections caused by multidrug-resistant Enterobacterales. This multicenter study aimed to investigate the performance of routine temocillin testing assays against Enterobacterales challenging strains. Forty-seven selected clinical isolates were blindly analyzed by 12 Belgian laboratories using VITEK® 2 (n = 5) and BD Phoenix™ (n = 3) automated systems, ETEST® gradient strip (n = 3), and disk (3 brands) diffusion method (DD; n = 6) for temocillin susceptibility using standardized methodology. Results were interpreted using EUCAST 2023 criteria and compared to the broth microdilution (BMD; Sensititre™ panel) method used as gold standard. Methods' reproducibility was assessed by testing 3 reference strains in triplicate. A total of 702 organism-drug results were obtained against 33 TMO-susceptible and 14 TMO-resistant isolates. Excluding Proteae species (P. mirabilis and M. morganii), the essential agreement rates were excellent (91.5-100%) for all MIC-based methods. The highest category agreement was achieved by ETEST® (97.5%) followed by VITEK® 2 (93.2%), disk diffusion (91.6%), and BD Phoenix™ (88.5%). BD Phoenix™ and paper disk diffusion overcalled resistance (11.5% and 6.8% of major discrepancies, respectively), while ROSCO tablets diffusion and VITEK® 2 generated higher very major discrepancies (7.1% and 4.2% respectively). Inter-assay reproducibility was unsatisfactory using recommended E. coli ATCC 25922 strain but was excellent with E. coli ATCC 35218 and K. pneumoniae ATCC 700603 strains. This interlaboratory study suggests that routine testing methods provide accurate and reproducible TMO categorization results except for Proteae species | ||
| 650 | 4 | |a Multicenter Study | |
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Antimicrobial susceptibility testing | |
| 650 | 4 | |a EUCAST Breakpoints | |
| 650 | 4 | |a Enterobacterales | |
| 650 | 4 | |a Temocillin | |
| 650 | 7 | |a temocillin |2 NLM | |
| 650 | 7 | |a 03QB156W6I |2 NLM | |
| 650 | 7 | |a Anti-Bacterial Agents |2 NLM | |
| 650 | 7 | |a Penicillins |2 NLM | |
| 700 | 1 | |a Bélik, Florian |e verfasserin |4 aut | |
| 700 | 1 | |a Denis, Olivier |e verfasserin |4 aut | |
| 700 | 1 | |a Montesinos, Isabel |e verfasserin |4 aut | |
| 700 | 1 | |a Bogaerts, Pierre |e verfasserin |4 aut | |
| 700 | 1 | |a Boelens, Jerina |e verfasserin |4 aut | |
| 700 | 1 | |a Brassinne, Laetitia |e verfasserin |4 aut | |
| 700 | 1 | |a Descy, Julie |e verfasserin |4 aut | |
| 700 | 1 | |a Desmet, Stefanie |e verfasserin |4 aut | |
| 700 | 1 | |a Gils, Sarah |e verfasserin |4 aut | |
| 700 | 1 | |a Lissoir, Bénédicte |e verfasserin |4 aut | |
| 700 | 1 | |a Magerman, Koen |e verfasserin |4 aut | |
| 700 | 1 | |a Matheeussen, Veerle |e verfasserin |4 aut | |
| 700 | 1 | |a Meex, Cécile |e verfasserin |4 aut | |
| 700 | 1 | |a Rodriguez Villalobos, Hector |e verfasserin |4 aut | |
| 700 | 1 | |a Van den Abeele, Anne Marie |e verfasserin |4 aut | |
| 700 | 1 | |a Vernelen, Kris |e verfasserin |4 aut | |
| 700 | 1 | |a Ceyssens, Pieter-Jan |e verfasserin |4 aut | |
| 700 | 1 | |a Huang, Te-Din |e verfasserin |4 aut | |
| 700 | 0 | |a Belgian National Antibiogram Committee |e verfasserin |4 aut | |
| 700 | 1 | |a Boelens, Jerina |e investigator |4 oth | |
| 700 | 1 | |a Brassinne, Laetitia |e investigator |4 oth | |
| 700 | 1 | |a Catteau, Lucy |e investigator |4 oth | |
| 700 | 1 | |a Ceyssens, Pieter-Jan |e investigator |4 oth | |
| 700 | 1 | |a Descy, Julie |e investigator |4 oth | |
| 700 | 1 | |a Desmet, Stefanie |e investigator |4 oth | |
| 700 | 1 | |a Gils, Sarah |e investigator |4 oth | |
| 700 | 1 | |a Latour, Katrien |e investigator |4 oth | |
| 700 | 1 | |a Lissoir, Bénédicte |e investigator |4 oth | |
| 700 | 1 | |a Magerman, Koen |e investigator |4 oth | |
| 700 | 1 | |a Matheeussen, Veerle |e investigator |4 oth | |
| 700 | 1 | |a Meex, Cécile |e investigator |4 oth | |
| 700 | 1 | |a Rodriguez Villalobos, Hector |e investigator |4 oth | |
| 700 | 1 | |a Vandamme, Sarah |e investigator |4 oth | |
| 700 | 1 | |a Van den Abeele, Anne-Marie |e investigator |4 oth | |
| 700 | 1 | |a Vilain, Aline |e investigator |4 oth | |
| 700 | 1 | |a Vernelen, Kris |e investigator |4 oth | |
| 700 | 1 | |a Wybo, Ingrid |e investigator |4 oth | |
| 700 | 1 | |a Yaras, Harun |e investigator |4 oth | |
| 700 | 1 | |a Yin, Nicolas |e investigator |4 oth | |
| 773 | 0 | 8 | |i Enthalten in |t European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology |d 1988 |g 42(2023), 12 vom: 01. Dez., Seite 1477-1483 |w (DE-627)NLM012620742 |x 1435-4373 |7 nnns |
| 773 | 1 | 8 | |g volume:42 |g year:2023 |g number:12 |g day:01 |g month:12 |g pages:1477-1483 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1007/s10096-023-04681-y |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 42 |j 2023 |e 12 |b 01 |c 12 |h 1477-1483 | ||