Phenylalanine-Arginine-β-Naphthylamide Enhances the Photobactericidal Effect of Methylene Blue on Pseudomonas aeruginosa
Objective: To investigate the effectiveness, dosing sequence, concentration, and mechanism of antimicrobial photodynamic inactivation (aPDI) using methylene blue (MB) plus phenylalanine-arginine-β-naphthylamide (PAβN) against Pseudomonas aeruginosa. Methods: P. aeruginosa bacterial suspension was incubated with MB for different times (5-240 min), and then, 10 J/cm2 red light was irradiated. The efflux pump inhibitor (EPI) PAβN (10-100 μg/mL) was combined with MB (1-20 μM) in different sequences (PAβN-first, PAβN+MB, PAβN-after). Colony-forming units were then determined by serial dilution. Results: Using MB 10 μM plus 10 J/cm2, the killing effect of MB-aPDI on P. aeruginosa increased first and then decreased with longer incubation time. The killing effect of MB+PAβN-aPDI on P. aeruginosa was better than that of MB-aPDI (p < 0.05) by up to 2 logs. PAβN-first had the best killing effect, whereas PAβN-after had the worst killing effect. The killing effect increased with PAβN concentration and at 100 μg/mL reached 5.1 logs. Conclusions: The EPI PAβN enhanced the bactericidal effect of MB-aPDI on P. aeruginosa, especially when added before MB. It is proposed that MB is a substrate of the resistance-nodulation-division family efflux pump.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:41 |
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| Enthalten in: |
Photobiomodulation, photomedicine, and laser surgery - 41(2023), 10 vom: 02. Okt., Seite 569-575 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
He, Lixia [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 01.11.2023 Date Revised 01.11.2023 published: Print Citation Status MEDLINE |
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| doi: |
10.1089/photob.2023.0005 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM363627219 |
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| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Objective: To investigate the effectiveness, dosing sequence, concentration, and mechanism of antimicrobial photodynamic inactivation (aPDI) using methylene blue (MB) plus phenylalanine-arginine-β-naphthylamide (PAβN) against Pseudomonas aeruginosa. Methods: P. aeruginosa bacterial suspension was incubated with MB for different times (5-240 min), and then, 10 J/cm2 red light was irradiated. The efflux pump inhibitor (EPI) PAβN (10-100 μg/mL) was combined with MB (1-20 μM) in different sequences (PAβN-first, PAβN+MB, PAβN-after). Colony-forming units were then determined by serial dilution. Results: Using MB 10 μM plus 10 J/cm2, the killing effect of MB-aPDI on P. aeruginosa increased first and then decreased with longer incubation time. The killing effect of MB+PAβN-aPDI on P. aeruginosa was better than that of MB-aPDI (p < 0.05) by up to 2 logs. PAβN-first had the best killing effect, whereas PAβN-after had the worst killing effect. The killing effect increased with PAβN concentration and at 100 μg/mL reached 5.1 logs. Conclusions: The EPI PAβN enhanced the bactericidal effect of MB-aPDI on P. aeruginosa, especially when added before MB. It is proposed that MB is a substrate of the resistance-nodulation-division family efflux pump | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Pseudomonas aeruginosa | |
| 650 | 4 | |a antimicrobial photodynamic inactivation | |
| 650 | 4 | |a efflux pump inhibitor | |
| 650 | 4 | |a methylene blue | |
| 650 | 4 | |a phenylalanine-arginine-β-naphthylamide | |
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| 650 | 7 | |a T42P99266K |2 NLM | |
| 650 | 7 | |a phenylalanine arginine beta-naphthylamide |2 NLM | |
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| 700 | 1 | |a Yang, Shanlin |e verfasserin |4 aut | |
| 700 | 1 | |a Xuan, Weijun |e verfasserin |4 aut | |
| 700 | 1 | |a Zhen, Xiumei |e verfasserin |4 aut | |
| 700 | 1 | |a Qi, Qi |e verfasserin |4 aut | |
| 700 | 1 | |a Qi, Yongqi |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Qingxiang |e verfasserin |4 aut | |
| 700 | 1 | |a Du, Meixia |e verfasserin |4 aut | |
| 700 | 1 | |a Hamblin, Michael R |e verfasserin |4 aut | |
| 700 | 1 | |a Huang, Liyi |e verfasserin |4 aut | |
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