Adaptation to metal(loid)s in strain Mucilaginibacter rubeus P2 involves novel arsenic resistance genes and mechanisms
Copyright © 2023 Elsevier B.V. All rights reserved..
Arsenic is a ubiquitous environmental toxi substance that affects human health. Compared to inorganic arsenicals, reduced organoarsenicals are more toxic, and some of them are recognized as antibiotics, such as methylarsenite [MAs(III)] and arsinothricin (2-amino-4-(hydroxymethylarsinoyl)butanoate, or AST). To date, organoarsenicals such as MAs(V) and roxarsone [Rox(V)] are still used in agriculture and animal husbandry. How bacteria deal with both inorganic and organoarsenic species is unclear. Recently, we identified an environmental isolate Mucilaginibacter rubeus P2 that has adapted to high arsenic and antinomy levels by triplicating an arsR-mrarsUBact-arsN-arsC-(arsRhp)-hp-acr3-mrme1Bact-mrme2Bactgene cluster. Heterologous expression of mrarsMBact, mrarsUBact, mrme1Bact and mrme2Bact, encoding putative arsenic resistance determinants, in the arsenic hypersensitive strain Escherichia coli AW3110 conferred resistance to As(III), As(V), MAs(III) or Rox(III). Our data suggest that metalloid exposure promotes plasticity in arsenic resistance systems, enhancing host organism adaptation to metalloid stress.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2024 2023 |
|---|---|
| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:462 |
|---|---|
| Enthalten in: |
Journal of hazardous materials - 462(2023) vom: 15. Jan., Seite 132796 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Li, Yuanping [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
Adaptation |
|---|
| Anmerkungen: |
Date Completed 29.11.2023 Date Revised 07.12.2023 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1016/j.jhazmat.2023.132796 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM36357400X |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM36357400X | ||
| 003 | DE-627 | ||
| 005 | 20231226093633.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231226s2024 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.jhazmat.2023.132796 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1211.xml |
| 035 | |a (DE-627)NLM36357400X | ||
| 035 | |a (NLM)37865075 | ||
| 035 | |a (PII)S0304-3894(23)02080-0 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Li, Yuanping |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Adaptation to metal(loid)s in strain Mucilaginibacter rubeus P2 involves novel arsenic resistance genes and mechanisms |
| 264 | 1 | |c 2024 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 29.11.2023 | ||
| 500 | |a Date Revised 07.12.2023 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2023 Elsevier B.V. All rights reserved. | ||
| 520 | |a Arsenic is a ubiquitous environmental toxi substance that affects human health. Compared to inorganic arsenicals, reduced organoarsenicals are more toxic, and some of them are recognized as antibiotics, such as methylarsenite [MAs(III)] and arsinothricin (2-amino-4-(hydroxymethylarsinoyl)butanoate, or AST). To date, organoarsenicals such as MAs(V) and roxarsone [Rox(V)] are still used in agriculture and animal husbandry. How bacteria deal with both inorganic and organoarsenic species is unclear. Recently, we identified an environmental isolate Mucilaginibacter rubeus P2 that has adapted to high arsenic and antinomy levels by triplicating an arsR-mrarsUBact-arsN-arsC-(arsRhp)-hp-acr3-mrme1Bact-mrme2Bactgene cluster. Heterologous expression of mrarsMBact, mrarsUBact, mrme1Bact and mrme2Bact, encoding putative arsenic resistance determinants, in the arsenic hypersensitive strain Escherichia coli AW3110 conferred resistance to As(III), As(V), MAs(III) or Rox(III). Our data suggest that metalloid exposure promotes plasticity in arsenic resistance systems, enhancing host organism adaptation to metalloid stress | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, N.I.H., Extramural | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Adaptation | |
| 650 | 4 | |a Arsenic | |
| 650 | 4 | |a Mucilaginibacter | |
| 650 | 4 | |a Novel ars genes | |
| 650 | 7 | |a Arsenic |2 NLM | |
| 650 | 7 | |a N712M78A8G |2 NLM | |
| 650 | 7 | |a Arsenicals |2 NLM | |
| 650 | 7 | |a Roxarsone |2 NLM | |
| 650 | 7 | |a H5GU9YQL7L |2 NLM | |
| 700 | 1 | |a Yu, Yanshuang |e verfasserin |4 aut | |
| 700 | 1 | |a Yang, Xiaojun |e verfasserin |4 aut | |
| 700 | 1 | |a Pat-Espadas, Aurora M |e verfasserin |4 aut | |
| 700 | 1 | |a Vinuesa, Pablo |e verfasserin |4 aut | |
| 700 | 1 | |a Herzberg, Martin |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Jian |e verfasserin |4 aut | |
| 700 | 1 | |a Rosen, Barry P |e verfasserin |4 aut | |
| 700 | 1 | |a Feng, Renwei |e verfasserin |4 aut | |
| 700 | 1 | |a Rensing, Christopher |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Journal of hazardous materials |d 1998 |g 462(2023) vom: 15. Jan., Seite 132796 |w (DE-627)NLM093662688 |x 1873-3336 |7 nnns |
| 773 | 1 | 8 | |g volume:462 |g year:2023 |g day:15 |g month:01 |g pages:132796 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1016/j.jhazmat.2023.132796 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 462 |j 2023 |b 15 |c 01 |h 132796 | ||