Profilin1 is required for prevention of mitotic catastrophe in murine and human glomerular diseases
The progression of proteinuric kidney diseases is associated with podocyte loss, but the mechanisms underlying this process remain unclear. Podocytes reenter the cell cycle to repair double-stranded DNA breaks. However, unsuccessful repair can result in podocytes crossing the G1/S checkpoint and undergoing abortive cytokinesis. In this study, we identified Pfn1 as indispensable in maintaining glomerular integrity - its tissue-specific loss in mouse podocytes resulted in severe proteinuria and kidney failure. Our results suggest that this phenotype is due to podocyte mitotic catastrophe (MC), characterized histologically and ultrastructurally by abundant multinucleated cells, irregular nuclei, and mitotic spindles. Podocyte cell cycle reentry was identified using FUCCI2aR mice, and we observed altered expression of cell-cycle associated proteins, such as p21, p53, cyclin B1, and cyclin D1. Podocyte-specific translating ribosome affinity purification and RNA-Seq revealed the downregulation of ribosomal RNA-processing 8 (Rrp8). Overexpression of Rrp8 in Pfn1-KO podocytes partially rescued the phenotype in vitro. Clinical and ultrastructural tomographic analysis of patients with diverse proteinuric kidney diseases further validated the presence of MC podocytes and reduction in podocyte PFN1 expression within kidney tissues. These results suggest that profilin1 is essential in regulating the podocyte cell cycle and its disruption leads to MC and subsequent podocyte loss.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:133 |
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| Enthalten in: |
The Journal of clinical investigation - 133(2023), 24 vom: 15. Dez. |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Tian, Xuefei [VerfasserIn] |
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| Links: |
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| Themen: |
Cell Biology |
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| Anmerkungen: |
Date Completed 26.12.2023 Date Revised 07.03.2024 published: Electronic Citation Status MEDLINE |
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| doi: |
10.1172/JCI171237 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM363401164 |
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| 100 | 1 | |a Tian, Xuefei |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Profilin1 is required for prevention of mitotic catastrophe in murine and human glomerular diseases |
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| 500 | |a Date Revised 07.03.2024 | ||
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| 500 | |a Citation Status MEDLINE | ||
| 520 | |a The progression of proteinuric kidney diseases is associated with podocyte loss, but the mechanisms underlying this process remain unclear. Podocytes reenter the cell cycle to repair double-stranded DNA breaks. However, unsuccessful repair can result in podocytes crossing the G1/S checkpoint and undergoing abortive cytokinesis. In this study, we identified Pfn1 as indispensable in maintaining glomerular integrity - its tissue-specific loss in mouse podocytes resulted in severe proteinuria and kidney failure. Our results suggest that this phenotype is due to podocyte mitotic catastrophe (MC), characterized histologically and ultrastructurally by abundant multinucleated cells, irregular nuclei, and mitotic spindles. Podocyte cell cycle reentry was identified using FUCCI2aR mice, and we observed altered expression of cell-cycle associated proteins, such as p21, p53, cyclin B1, and cyclin D1. Podocyte-specific translating ribosome affinity purification and RNA-Seq revealed the downregulation of ribosomal RNA-processing 8 (Rrp8). Overexpression of Rrp8 in Pfn1-KO podocytes partially rescued the phenotype in vitro. Clinical and ultrastructural tomographic analysis of patients with diverse proteinuric kidney diseases further validated the presence of MC podocytes and reduction in podocyte PFN1 expression within kidney tissues. These results suggest that profilin1 is essential in regulating the podocyte cell cycle and its disruption leads to MC and subsequent podocyte loss | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Cell Biology | |
| 650 | 4 | |a Cell cycle | |
| 650 | 4 | |a Nephrology | |
| 650 | 7 | |a Cell Cycle Proteins |2 NLM | |
| 650 | 7 | |a PFN1 protein, human |2 NLM | |
| 650 | 7 | |a Profilins |2 NLM | |
| 650 | 7 | |a Pfn1 protein, mouse |2 NLM | |
| 700 | 1 | |a Pedigo, Christopher E |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Ke |e verfasserin |4 aut | |
| 700 | 1 | |a Ma, Xiaotao |e verfasserin |4 aut | |
| 700 | 1 | |a Bunda, Patricia |e verfasserin |4 aut | |
| 700 | 1 | |a Pell, John |e verfasserin |4 aut | |
| 700 | 1 | |a Lek, Angela |e verfasserin |4 aut | |
| 700 | 1 | |a Gu, Jianlei |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Yan |e verfasserin |4 aut | |
| 700 | 1 | |a Medina Rangel, Paulina X |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Wei |e verfasserin |4 aut | |
| 700 | 1 | |a Schwartze, Eike |e verfasserin |4 aut | |
| 700 | 1 | |a Nagata, Soichiro |e verfasserin |4 aut | |
| 700 | 1 | |a Lerner, Gabriel |e verfasserin |4 aut | |
| 700 | 1 | |a Perincheri, Sudhir |e verfasserin |4 aut | |
| 700 | 1 | |a Priyadarshini, Anupama |e verfasserin |4 aut | |
| 700 | 1 | |a Zhao, Hongyu |e verfasserin |4 aut | |
| 700 | 1 | |a Lek, Monkol |e verfasserin |4 aut | |
| 700 | 1 | |a Menon, Madhav C |e verfasserin |4 aut | |
| 700 | 1 | |a Fu, Rongguo |e verfasserin |4 aut | |
| 700 | 1 | |a Ishibe, Shuta |e verfasserin |4 aut | |
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