A comprehensive evaluation of a polymeric zwitterionic hydrophilic monolith for nucleotide separation
© 2023. The Author(s), under exclusive licence to The Japan Society for Analytical Chemistry..
Rapid and effective separation of nucleotides (NTs) and their derivatives is crucial for studying their physiological functions. In this work, we comprehensively evaluated the separation ability of a zwitterionic hydrophilic monolith, i.e., poly(N,N-dimethyl-N-(3-methacrylamidopropyl)-N-(3-sulfopropyl)ammonium betaine-co-N,N'-methylenebisacrylamide) (poly(SPP-co-MBA)) for NTs analysis, including its selectivity, chemical stability under extremely basic condition and compatibility with hydrophilic interaction liquid chromatography (HILIC) coupled with mass spectrometry (HILIC-MS). The poly(SPP-co-MBA) monolith exhibited excellent chemical stability, as evidenced by the low relative standard deviation of retention time (0.16-1.05%) after 4000 consecutive injections over one month under strong alkaline elution condition (pH 10). After optimizing the separation conditions, including buffer pH and concentration, organic solvent content and column temperature, four nucleoside triphosphates, five nucleoside diphosphates and five nucleoside monophosphates were baseline separated within 7 min. Additionally, the mixtures containing one nucleoside and its corresponding mono-, di-, and triphosphates were baseline separated within only 3 min, respectively. It is good HILIC-MS compatibility was also confirmed by the satisfactory peak shape and high response of nine NTs. Overall, the proposed poly(SPP-co-MBA) monolith exhibited good mechanical stability and compatibility of HILIC-MS, making it a promising technique for NTs analysis.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:40 |
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| Enthalten in: |
Analytical sciences : the international journal of the Japan Society for Analytical Chemistry - 40(2024), 1 vom: 24. Jan., Seite 85-91 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Lai, Liang [VerfasserIn] |
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| Links: |
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| Themen: |
3SCV180C9W |
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| Anmerkungen: |
Date Completed 05.01.2024 Date Revised 25.01.2024 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1007/s44211-023-00430-5 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM363365370 |
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| 520 | |a Rapid and effective separation of nucleotides (NTs) and their derivatives is crucial for studying their physiological functions. In this work, we comprehensively evaluated the separation ability of a zwitterionic hydrophilic monolith, i.e., poly(N,N-dimethyl-N-(3-methacrylamidopropyl)-N-(3-sulfopropyl)ammonium betaine-co-N,N'-methylenebisacrylamide) (poly(SPP-co-MBA)) for NTs analysis, including its selectivity, chemical stability under extremely basic condition and compatibility with hydrophilic interaction liquid chromatography (HILIC) coupled with mass spectrometry (HILIC-MS). The poly(SPP-co-MBA) monolith exhibited excellent chemical stability, as evidenced by the low relative standard deviation of retention time (0.16-1.05%) after 4000 consecutive injections over one month under strong alkaline elution condition (pH 10). After optimizing the separation conditions, including buffer pH and concentration, organic solvent content and column temperature, four nucleoside triphosphates, five nucleoside diphosphates and five nucleoside monophosphates were baseline separated within 7 min. Additionally, the mixtures containing one nucleoside and its corresponding mono-, di-, and triphosphates were baseline separated within only 3 min, respectively. It is good HILIC-MS compatibility was also confirmed by the satisfactory peak shape and high response of nine NTs. Overall, the proposed poly(SPP-co-MBA) monolith exhibited good mechanical stability and compatibility of HILIC-MS, making it a promising technique for NTs analysis | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Nucleotides separation | |
| 650 | 4 | |a Polymeric monolith | |
| 650 | 4 | |a Rapid separation | |
| 650 | 4 | |a Strong alkali condition | |
| 650 | 4 | |a Zwitterionic hydrophilic chromatography | |
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| 650 | 7 | |a Betaine |2 NLM | |
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| 700 | 1 | |a Zhang, Mengyun |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Chusheng |e verfasserin |4 aut | |
| 700 | 1 | |a Qu, Jiahuan |e verfasserin |4 aut | |
| 700 | 1 | |a Xu, Dongsheng |e verfasserin |4 aut | |
| 700 | 1 | |a Jiang, Zhengjin |e verfasserin |4 aut | |
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