Global analysis of seed transcriptomes reveals a novel PLATZ regulator for seed size and weight control in soybean

© 2023 The Authors New Phytologist © 2023 New Phytologist Foundation..

Seed size and weight are important factors that influence soybean yield. Combining the weighted gene co-expression network analysis (WGCNA) of 45 soybean accessions and gene dynamic changes in seeds at seven developmental stages, we identified candidate genes that may control the seed size/weight. Among these, a PLATZ-type regulator overlapping with 10 seed weight QTLs was further investigated. This zinc-finger transcriptional regulator, named as GmPLATZ, is required for the promotion of seed size and weight in soybean. The GmPLATZ may exert its functions through direct binding to the promoters and activation of the expression of cyclin genes and GmGA20OX for cell proliferation. Overexpression of the GmGA20OX enhanced seed size/weight in soybean. We further found that the GmPLATZ binds to a 32-bp sequence containing a core palindromic element AATGCGCATT. Spacing of the flanking sequences beyond the core element facilitated GmPLATZ binding. An elite haplotype Hap3 was also identified to have higher promoter activity and correlated with higher gene expression and higher seed weight. Orthologues of the GmPLATZ from rice and Arabidopsis play similar roles in seeds. Our study reveals a novel module of GmPLATZ-GmGA20OX/cyclins in regulating seed size and weight and provides valuable targets for breeding of crops with desirable agronomic traits.

Medienart:

E-Artikel

Erscheinungsjahr:

2023

Erschienen:

2023

Enthalten in:

Zur Gesamtaufnahme - volume:240

Enthalten in:

The New phytologist - 240(2023), 6 vom: 30. Dez., Seite 2436-2454

Sprache:

Englisch

Beteiligte Personen:

Hu, Yang [VerfasserIn]
Liu, Yue [VerfasserIn]
Lu, Long [VerfasserIn]
Tao, Jian-Jun [VerfasserIn]
Cheng, Tong [VerfasserIn]
Jin, Meng [VerfasserIn]
Wang, Zhou-Ya [VerfasserIn]
Wei, Jun-Jie [VerfasserIn]
Jiang, Zhi-Hao [VerfasserIn]
Sun, Wan-Cai [VerfasserIn]
Liu, Cheng-Lan [VerfasserIn]
Gao, Feng [VerfasserIn]
Zhang, Yong [VerfasserIn]
Li, Wei [VerfasserIn]
Bi, Ying-Dong [VerfasserIn]
Lai, Yong-Cai [VerfasserIn]
Zhou, Bin [VerfasserIn]
Yu, De-Yue [VerfasserIn]
Yin, Cui-Cui [VerfasserIn]
Wei, Wei [VerfasserIn]
Zhang, Wan-Ke [VerfasserIn]
Chen, Shou-Yi [VerfasserIn]
Zhang, Jin-Song [VerfasserIn]

Links:

Volltext

Themen:

Cell cycle
Gibberellin
Journal Article
PLATZ
Seed size
Seed weight
Soybean

Anmerkungen:

Date Completed 17.11.2023

Date Revised 13.12.2023

published: Print-Electronic

Citation Status MEDLINE

doi:

10.1111/nph.19316

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM363331786