Liposomes - Human phagocytes interplay in whole blood : effect of liposome design
Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved..
Nanomedicine holds immense potential for therapeutic manipulation of phagocytic immune cells. However, in vitro studies often fail to accurately translate to the complex in vivo environment. To address this gap, we employed an ex vivo human whole-blood assay to evaluate liposome interactions with immune cells. We systematically varied liposome size, PEG-surface densities and sphingomyelin and ganglioside content. We observed differential uptake patterns of the assessed liposomes by neutrophils and monocytes, emphasizing the importance of liposome design. Interestingly, our results aligned closely with published in vivo observations in mice and patients. Moreover, liposome exposure induced changes in cytokine release and cellular responses, highlighting the potential modulation of immune system. Our study highlights the utility of human whole-blood models in assessing nanoparticle-immune cell interactions and provides insights into liposome design for modulating immune responses.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2023 |
|---|---|
| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:54 |
|---|---|
| Enthalten in: |
Nanomedicine : nanotechnology, biology, and medicine - 54(2023) vom: 12. Nov., Seite 102712 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Giambelluca, Miriam [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
Cytokines |
|---|
| Anmerkungen: |
Date Completed 06.11.2023 Date Revised 06.11.2023 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1016/j.nano.2023.102712 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM363310568 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM363310568 | ||
| 003 | DE-627 | ||
| 005 | 20231226093052.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231226s2023 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.nano.2023.102712 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1210.xml |
| 035 | |a (DE-627)NLM363310568 | ||
| 035 | |a (NLM)37838100 | ||
| 035 | |a (PII)S1549-9634(23)00063-1 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Giambelluca, Miriam |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Liposomes - Human phagocytes interplay in whole blood |b effect of liposome design |
| 264 | 1 | |c 2023 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 06.11.2023 | ||
| 500 | |a Date Revised 06.11.2023 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved. | ||
| 520 | |a Nanomedicine holds immense potential for therapeutic manipulation of phagocytic immune cells. However, in vitro studies often fail to accurately translate to the complex in vivo environment. To address this gap, we employed an ex vivo human whole-blood assay to evaluate liposome interactions with immune cells. We systematically varied liposome size, PEG-surface densities and sphingomyelin and ganglioside content. We observed differential uptake patterns of the assessed liposomes by neutrophils and monocytes, emphasizing the importance of liposome design. Interestingly, our results aligned closely with published in vivo observations in mice and patients. Moreover, liposome exposure induced changes in cytokine release and cellular responses, highlighting the potential modulation of immune system. Our study highlights the utility of human whole-blood models in assessing nanoparticle-immune cell interactions and provides insights into liposome design for modulating immune responses | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Human whole blood | |
| 650 | 4 | |a Liposomes | |
| 650 | 4 | |a Nanomedicine | |
| 650 | 4 | |a Nanotechnology | |
| 650 | 4 | |a PEGylation | |
| 650 | 7 | |a Liposomes |2 NLM | |
| 650 | 7 | |a Sphingomyelins |2 NLM | |
| 650 | 7 | |a Cytokines |2 NLM | |
| 700 | 1 | |a Markova, Elena |e verfasserin |4 aut | |
| 700 | 1 | |a Louet, Claire |e verfasserin |4 aut | |
| 700 | 1 | |a Steinkjer, Bjørg |e verfasserin |4 aut | |
| 700 | 1 | |a Sundset, Rune |e verfasserin |4 aut | |
| 700 | 1 | |a Škalko-Basnet, Nataša |e verfasserin |4 aut | |
| 700 | 1 | |a Hak, Sjoerd |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Nanomedicine : nanotechnology, biology, and medicine |d 2005 |g 54(2023) vom: 12. Nov., Seite 102712 |w (DE-627)NLM16062021X |x 1549-9642 |7 nnns |
| 773 | 1 | 8 | |g volume:54 |g year:2023 |g day:12 |g month:11 |g pages:102712 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1016/j.nano.2023.102712 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 54 |j 2023 |b 12 |c 11 |h 102712 | ||