Amino acid 17 in QRDR of Gyrase A plays a key role in fluoroquinolones susceptibility in mycobacteria
IMPORTANCE: Fluoroquinolones (FQs) play a key role in the treatment regimens against tuberculosis and non-tuberculous mycobacterial infections. However, there are significant differences in the sensitivities of different mycobacteria to FQs. In this study, we proved that this is associated with the polymorphism at amino acid 17 of quinolone resistance-determining region of Gyrase A by gene editing. This is the first study using CRISPR-associated recombination for gene editing in Mycobacterium abscessus to underscore the contribution of the amino acid substitutions in GyrA to FQ susceptibilities in mycobacteria.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:11 |
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| Enthalten in: |
Microbiology spectrum - 11(2023), 6 vom: 12. Dez., Seite e0280923 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Wang, Shuai [VerfasserIn] |
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| Links: |
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| Themen: |
Amino Acids |
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| Anmerkungen: |
Date Completed 16.12.2023 Date Revised 16.12.2023 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1128/spectrum.02809-23 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 520 | |a IMPORTANCE: Fluoroquinolones (FQs) play a key role in the treatment regimens against tuberculosis and non-tuberculous mycobacterial infections. However, there are significant differences in the sensitivities of different mycobacteria to FQs. In this study, we proved that this is associated with the polymorphism at amino acid 17 of quinolone resistance-determining region of Gyrase A by gene editing. This is the first study using CRISPR-associated recombination for gene editing in Mycobacterium abscessus to underscore the contribution of the amino acid substitutions in GyrA to FQ susceptibilities in mycobacteria | ||
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| 700 | 1 | |a Ding, Jie |e verfasserin |4 aut | |
| 700 | 1 | |a Guan, Ping |e verfasserin |4 aut | |
| 700 | 1 | |a Fang, Xiange |e verfasserin |4 aut | |
| 700 | 1 | |a Peng, Jiacong |e verfasserin |4 aut | |
| 700 | 1 | |a Su, Biyi |e verfasserin |4 aut | |
| 700 | 1 | |a Ma, Shangming |e verfasserin |4 aut | |
| 700 | 1 | |a Tan, Yaoju |e verfasserin |4 aut | |
| 700 | 1 | |a M Cook, Gregory |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Guoliang |e verfasserin |4 aut | |
| 700 | 1 | |a Lin, Yongping |e verfasserin |4 aut | |
| 700 | 1 | |a Zhong, Nanshan |e verfasserin |4 aut | |
| 700 | 1 | |a Hu, Jinxing |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Jianxiong |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Tianyu |e verfasserin |4 aut | |
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