Kilohertz serial crystallography with the JUNGFRAU detector at a fourth-generation synchrotron source
open access..
Serial and time-resolved macromolecular crystallography are on the rise. However, beam time at X-ray free-electron lasers is limited and most third-generation synchrotron-based macromolecular crystallography beamlines do not offer the necessary infrastructure yet. Here, a new setup is demonstrated, based on the JUNGFRAU detector and Jungfraujoch data-acquisition system, that enables collection of kilohertz serial crystallography data at fourth-generation synchrotrons. More importantly, it is shown that this setup is capable of collecting multiple-time-point time-resolved protein dynamics at kilohertz rates, allowing the probing of microsecond to second dynamics at synchrotrons in a fraction of the time needed previously. A high-quality complete X-ray dataset was obtained within 1 min from lysozyme microcrystals, and the dynamics of the light-driven sodium-pump membrane protein KR2 with a time resolution of 1 ms could be demonstrated. To make the setup more accessible for researchers, downstream data handling and analysis will be automated to allow on-the-fly spot finding and indexing, as well as data processing.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:10 |
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| Enthalten in: |
IUCrJ - 10(2023), Pt 6 vom: 01. Nov., Seite 729-737 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Leonarski, Filip [VerfasserIn] |
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| Links: |
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| Themen: |
Data networks |
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| Anmerkungen: |
Date Revised 03.11.2023 published: Electronic Citation Status PubMed-not-MEDLINE |
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| doi: |
10.1107/S2052252523008618 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM363237429 |
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| 100 | 1 | |a Leonarski, Filip |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Kilohertz serial crystallography with the JUNGFRAU detector at a fourth-generation synchrotron source |
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| 500 | |a Citation Status PubMed-not-MEDLINE | ||
| 520 | |a open access. | ||
| 520 | |a Serial and time-resolved macromolecular crystallography are on the rise. However, beam time at X-ray free-electron lasers is limited and most third-generation synchrotron-based macromolecular crystallography beamlines do not offer the necessary infrastructure yet. Here, a new setup is demonstrated, based on the JUNGFRAU detector and Jungfraujoch data-acquisition system, that enables collection of kilohertz serial crystallography data at fourth-generation synchrotrons. More importantly, it is shown that this setup is capable of collecting multiple-time-point time-resolved protein dynamics at kilohertz rates, allowing the probing of microsecond to second dynamics at synchrotrons in a fraction of the time needed previously. A high-quality complete X-ray dataset was obtained within 1 min from lysozyme microcrystals, and the dynamics of the light-driven sodium-pump membrane protein KR2 with a time resolution of 1 ms could be demonstrated. To make the setup more accessible for researchers, downstream data handling and analysis will be automated to allow on-the-fly spot finding and indexing, as well as data processing | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a JUNGFRAU detector | |
| 650 | 4 | |a X-ray detectors | |
| 650 | 4 | |a data networks | |
| 650 | 4 | |a fourth-generation synchrotrons | |
| 650 | 4 | |a macromolecular crystallography | |
| 650 | 4 | |a protein structure | |
| 650 | 4 | |a time-resolved serial crystallography | |
| 700 | 1 | |a Nan, Jie |e verfasserin |4 aut | |
| 700 | 1 | |a Matej, Zdenek |e verfasserin |4 aut | |
| 700 | 1 | |a Bertrand, Quentin |e verfasserin |4 aut | |
| 700 | 1 | |a Furrer, Antonia |e verfasserin |4 aut | |
| 700 | 1 | |a Gorgisyan, Ishkhan |e verfasserin |4 aut | |
| 700 | 1 | |a Bjelčić, Monika |e verfasserin |4 aut | |
| 700 | 1 | |a Kepa, Michal |e verfasserin |4 aut | |
| 700 | 1 | |a Glover, Hannah |e verfasserin |4 aut | |
| 700 | 1 | |a Hinger, Viktoria |e verfasserin |4 aut | |
| 700 | 1 | |a Eriksson, Thomas |e verfasserin |4 aut | |
| 700 | 1 | |a Cehovin, Aleksander |e verfasserin |4 aut | |
| 700 | 1 | |a Eguiraun, Mikel |e verfasserin |4 aut | |
| 700 | 1 | |a Gasparotto, Piero |e verfasserin |4 aut | |
| 700 | 1 | |a Mozzanica, Aldo |e verfasserin |4 aut | |
| 700 | 1 | |a Weinert, Tobias |e verfasserin |4 aut | |
| 700 | 1 | |a Gonzalez, Ana |e verfasserin |4 aut | |
| 700 | 1 | |a Standfuss, Jörg |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Meitian |e verfasserin |4 aut | |
| 700 | 1 | |a Ursby, Thomas |e verfasserin |4 aut | |
| 700 | 1 | |a Dworkowski, Florian |e verfasserin |4 aut | |
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