Experimental phasing opportunities for macromolecular crystallography at very long wavelengths
© 2023. Springer Nature Limited..
Despite recent advances in cryo-electron microscopy and artificial intelligence-based model predictions, a significant fraction of structure determinations by macromolecular crystallography still requires experimental phasing, usually by means of single-wavelength anomalous diffraction (SAD) techniques. Most synchrotron beamlines provide highly brilliant beams of X-rays of between 0.7 and 2 Å wavelength. Use of longer wavelengths to access the absorption edges of biologically important lighter atoms such as calcium, potassium, chlorine, sulfur and phosphorus for native-SAD phasing is attractive but technically highly challenging. The long-wavelength beamline I23 at Diamond Light Source overcomes these limitations and extends the accessible wavelength range to λ = 5.9 Å. Here we report 22 macromolecular structures solved in this extended wavelength range, using anomalous scattering from a range of elements which demonstrate the routine feasibility of lighter atom phasing. We suggest that, in light of its advantages, long-wavelength crystallography is a compelling option for experimental phasing.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:6 |
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| Enthalten in: |
Communications chemistry - 6(2023), 1 vom: 12. Okt., Seite 219 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
El Omari, Kamel [VerfasserIn] |
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| Anmerkungen: |
Date Revised 20.03.2024 published: Electronic Citation Status PubMed-not-MEDLINE |
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| doi: |
10.1038/s42004-023-01014-0 |
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| Förderinstitution / Projekttitel: |
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NLM363212663 |
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| 520 | |a Despite recent advances in cryo-electron microscopy and artificial intelligence-based model predictions, a significant fraction of structure determinations by macromolecular crystallography still requires experimental phasing, usually by means of single-wavelength anomalous diffraction (SAD) techniques. Most synchrotron beamlines provide highly brilliant beams of X-rays of between 0.7 and 2 Å wavelength. Use of longer wavelengths to access the absorption edges of biologically important lighter atoms such as calcium, potassium, chlorine, sulfur and phosphorus for native-SAD phasing is attractive but technically highly challenging. The long-wavelength beamline I23 at Diamond Light Source overcomes these limitations and extends the accessible wavelength range to λ = 5.9 Å. Here we report 22 macromolecular structures solved in this extended wavelength range, using anomalous scattering from a range of elements which demonstrate the routine feasibility of lighter atom phasing. We suggest that, in light of its advantages, long-wavelength crystallography is a compelling option for experimental phasing | ||
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| 700 | 1 | |a Orr, Christian M |e verfasserin |4 aut | |
| 700 | 1 | |a Latimer-Smith, Merlyn |e verfasserin |4 aut | |
| 700 | 1 | |a Winter, Graeme |e verfasserin |4 aut | |
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| 700 | 1 | |a Bountra, Kiran |e verfasserin |4 aut | |
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| 700 | 1 | |a Wittmann, Sina |e verfasserin |4 aut | |
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| 700 | 1 | |a Senda, Miki |e verfasserin |4 aut | |
| 700 | 1 | |a Matsugaki, Naohiro |e verfasserin |4 aut | |
| 700 | 1 | |a Kawano, Yoshiaki |e verfasserin |4 aut | |
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