Detecting DNA Loops Using Tethered Particle Motion
© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature..
The range of motion of a micron-sized bead tethered by a single polymer provides a dynamic readout of the effective length of the polymer. The excursions of the bead may reflect the intrinsic flexibility and/or topology of the polymer as well as changes due to the action activity of ligands that bind the polymer. This is a simple yet powerful experimental approach to investigate such interactions between DNA and proteins as demonstrated by experiments with the lac repressor. This protein forms a stable, tetrameric oligomer with two binding sites and can produce a loop of DNA between recognition sites separated along the length of a DNA molecule.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:2694 |
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| Enthalten in: |
Methods in molecular biology (Clifton, N.J.) - 2694(2024) vom: 04., Seite 451-466 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Qian, Jin [VerfasserIn] |
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| Links: |
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| Themen: |
9007-49-2 |
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| Anmerkungen: |
Date Completed 01.11.2023 Date Revised 05.03.2024 published: Print Citation Status MEDLINE |
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| doi: |
10.1007/978-1-0716-3377-9_21 |
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| funding: |
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| 520 | |a The range of motion of a micron-sized bead tethered by a single polymer provides a dynamic readout of the effective length of the polymer. The excursions of the bead may reflect the intrinsic flexibility and/or topology of the polymer as well as changes due to the action activity of ligands that bind the polymer. This is a simple yet powerful experimental approach to investigate such interactions between DNA and proteins as demonstrated by experiments with the lac repressor. This protein forms a stable, tetrameric oligomer with two binding sites and can produce a loop of DNA between recognition sites separated along the length of a DNA molecule | ||
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