Efficient clathrin-mediated entry of enteric adenoviruses in human duodenal cells
IMPORTANCE: Enteric adenoviruses have historically been difficult to grow in cell culture, which has resulted in lack of knowledge of host factors and pathways required for infection of these medically relevant viruses. Previous studies in non-intestinal cell lines showed slow infection kinetics and generated comparatively low virus yields compared to other adenovirus types. We suggest duodenum-derived HuTu80 cells as a superior cell line for studies to complement efforts using complex intestinal tissue models. We show that viral host cell factors required for virus entry differ between cell lines from distinct origins and demonstrate the importance of clathrin-mediated endocytosis.
Medienart: |
E-Artikel |
---|
Erscheinungsjahr: |
2023 |
---|---|
Erschienen: |
2023 |
Enthalten in: |
Zur Gesamtaufnahme - volume:97 |
---|---|
Enthalten in: |
Journal of virology - 97(2023), 10 vom: 31. Okt., Seite e0077023 |
Sprache: |
Englisch |
---|
Beteiligte Personen: |
Becker, Miriam [VerfasserIn] |
---|
Links: |
---|
Themen: |
Clathrin |
---|
Anmerkungen: |
Date Completed 04.01.2024 Date Revised 12.02.2024 published: Print-Electronic Citation Status MEDLINE |
---|
doi: |
10.1128/jvi.00770-23 |
---|
funding: |
|
---|---|
Förderinstitution / Projekttitel: |
|
PPN (Katalog-ID): |
NLM363166882 |
---|
LEADER | 01000caa a22002652 4500 | ||
---|---|---|---|
001 | NLM363166882 | ||
003 | DE-627 | ||
005 | 20240212231932.0 | ||
007 | cr uuu---uuuuu | ||
008 | 231226s2023 xx |||||o 00| ||eng c | ||
024 | 7 | |a 10.1128/jvi.00770-23 |2 doi | |
028 | 5 | 2 | |a pubmed24n1289.xml |
035 | |a (DE-627)NLM363166882 | ||
035 | |a (NLM)37823645 | ||
040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
041 | |a eng | ||
100 | 1 | |a Becker, Miriam |e verfasserin |4 aut | |
245 | 1 | 0 | |a Efficient clathrin-mediated entry of enteric adenoviruses in human duodenal cells |
264 | 1 | |c 2023 | |
336 | |a Text |b txt |2 rdacontent | ||
337 | |a ƒaComputermedien |b c |2 rdamedia | ||
338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
500 | |a Date Completed 04.01.2024 | ||
500 | |a Date Revised 12.02.2024 | ||
500 | |a published: Print-Electronic | ||
500 | |a Citation Status MEDLINE | ||
520 | |a IMPORTANCE: Enteric adenoviruses have historically been difficult to grow in cell culture, which has resulted in lack of knowledge of host factors and pathways required for infection of these medically relevant viruses. Previous studies in non-intestinal cell lines showed slow infection kinetics and generated comparatively low virus yields compared to other adenovirus types. We suggest duodenum-derived HuTu80 cells as a superior cell line for studies to complement efforts using complex intestinal tissue models. We show that viral host cell factors required for virus entry differ between cell lines from distinct origins and demonstrate the importance of clathrin-mediated endocytosis | ||
650 | 4 | |a Journal Article | |
650 | 4 | |a Research Support, Non-U.S. Gov't | |
650 | 4 | |a clathrin-mediated endocytosis | |
650 | 4 | |a electron microscopy | |
650 | 4 | |a enteric adenovirus | |
650 | 4 | |a single particle tracking | |
650 | 4 | |a virus entry | |
650 | 7 | |a Clathrin |2 NLM | |
700 | 1 | |a Conca, Dario Valter |e verfasserin |4 aut | |
700 | 1 | |a Dorma, Noemi |e verfasserin |4 aut | |
700 | 1 | |a Mistry, Nitesh |e verfasserin |4 aut | |
700 | 1 | |a Hahlin, Elin |e verfasserin |4 aut | |
700 | 1 | |a Frängsmyr, Lars |e verfasserin |4 aut | |
700 | 1 | |a Bally, Marta |e verfasserin |4 aut | |
700 | 1 | |a Arnberg, Niklas |e verfasserin |4 aut | |
700 | 1 | |a Gerold, Gisa |e verfasserin |4 aut | |
773 | 0 | 8 | |i Enthalten in |t Journal of virology |d 1967 |g 97(2023), 10 vom: 31. Okt., Seite e0077023 |w (DE-627)NLM000006866 |x 1098-5514 |7 nnns |
773 | 1 | 8 | |g volume:97 |g year:2023 |g number:10 |g day:31 |g month:10 |g pages:e0077023 |
856 | 4 | 0 | |u http://dx.doi.org/10.1128/jvi.00770-23 |3 Volltext |
912 | |a GBV_USEFLAG_A | ||
912 | |a GBV_NLM | ||
951 | |a AR | ||
952 | |d 97 |j 2023 |e 10 |b 31 |c 10 |h e0077023 |