Single-cell RNA sequencing of bronchoscopy specimens : development of a rapid minimal handling protocol
Single-cell RNA sequencing (scRNA-seq) is an important tool for understanding disease pathophysiology, including airway diseases. Currently, the majority of scRNA-seq studies in airway diseases have used invasive methods (airway biopsy, surgical resection), which carry inherent risks and thus present a major limitation to scRNA-seq investigation of airway pathobiology. Bronchial brushing, where the airway mucosa is sampled using a cytological brush, is a viable, less invasive method of obtaining airway cells for scRNA-seq. Here we describe the development of a rapid and minimal handling protocol for preparing single-cell suspensions from bronchial brush specimens for scRNA-seq. Our optimized protocol maximizes cell recovery and cell quality and facilitates large-scale profiling of the airway transcriptome at single-cell resolution.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:75 |
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| Enthalten in: |
BioTechniques - 75(2023), 4 vom: 24. Okt., Seite 157-167 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Gerayeli, Firoozeh V [VerfasserIn] |
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| Links: |
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| Themen: |
Airway diseases |
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| Anmerkungen: |
Date Completed 01.11.2023 Date Revised 17.11.2023 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.2144/btn-2023-0017 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM363089691 |
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| 520 | |a Single-cell RNA sequencing (scRNA-seq) is an important tool for understanding disease pathophysiology, including airway diseases. Currently, the majority of scRNA-seq studies in airway diseases have used invasive methods (airway biopsy, surgical resection), which carry inherent risks and thus present a major limitation to scRNA-seq investigation of airway pathobiology. Bronchial brushing, where the airway mucosa is sampled using a cytological brush, is a viable, less invasive method of obtaining airway cells for scRNA-seq. Here we describe the development of a rapid and minimal handling protocol for preparing single-cell suspensions from bronchial brush specimens for scRNA-seq. Our optimized protocol maximizes cell recovery and cell quality and facilitates large-scale profiling of the airway transcriptome at single-cell resolution | ||
| 650 | 4 | |a Journal Article | |
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| 700 | 1 | |a Yang, Chen Xi |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Xuan |e verfasserin |4 aut | |
| 700 | 1 | |a Guinto, Elizabeth |e verfasserin |4 aut | |
| 700 | 1 | |a Yang, Julia Shun-Wei |e verfasserin |4 aut | |
| 700 | 1 | |a Cheung, Chung Yan |e verfasserin |4 aut | |
| 700 | 1 | |a Stach, Tara R |e verfasserin |4 aut | |
| 700 | 1 | |a Shaipanich, Tawimas |e verfasserin |4 aut | |
| 700 | 1 | |a Leung, Janice M |e verfasserin |4 aut | |
| 700 | 1 | |a Sin, Don D |e verfasserin |4 aut | |
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