Visualization of a Cell Wall Hydrolase Inhibitor in Fusobacterium nucleatum by Immunofluorescence Microscopy
© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature..
Innately present in tears, saliva and mucosal secretions, lysozyme provides a critical defensive strategy to the host by cleaving the β-1,4-glycosidic bonds between N-acetylmuramic acid and N-acetyl-D-glucosamine residues of peptidoglycan of invading bacteria, leading to bacterial lysis. To counter this class of cell wall hydrolase enzymes, bacteria produce several lysozyme inhibitors, a representative of which, MliC, was identified in Escherichia coli, Pseudomonas aeruginosa, and various bacterial species. The Gram-negative oral anaerobe Fusobacterium nucleatum encodes an uncharacterized lipoprotein homologous to MliC, whose localization is unknown. Here, we provide an experimental procedure to localize this MliC-like lipoprotein by employing immunofluorescence microscopy. In principle, this protocol can be used for any bacterial system to monitor protein localization.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:2727 |
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| Enthalten in: |
Methods in molecular biology (Clifton, N.J.) - 2727(2024) vom: 01., Seite 27-33 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
To, Kevin [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 01.11.2023 Date Revised 25.03.2024 published: Print Citation Status MEDLINE |
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| doi: |
10.1007/978-1-0716-3491-2_3 |
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| funding: |
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| 520 | |a Innately present in tears, saliva and mucosal secretions, lysozyme provides a critical defensive strategy to the host by cleaving the β-1,4-glycosidic bonds between N-acetylmuramic acid and N-acetyl-D-glucosamine residues of peptidoglycan of invading bacteria, leading to bacterial lysis. To counter this class of cell wall hydrolase enzymes, bacteria produce several lysozyme inhibitors, a representative of which, MliC, was identified in Escherichia coli, Pseudomonas aeruginosa, and various bacterial species. The Gram-negative oral anaerobe Fusobacterium nucleatum encodes an uncharacterized lipoprotein homologous to MliC, whose localization is unknown. Here, we provide an experimental procedure to localize this MliC-like lipoprotein by employing immunofluorescence microscopy. In principle, this protocol can be used for any bacterial system to monitor protein localization | ||
| 650 | 4 | |a Journal Article | |
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| 650 | 4 | |a Cell wall hydrolase | |
| 650 | 4 | |a Fusobacterium nucleatum | |
| 650 | 4 | |a Gram-negative bacteria | |
| 650 | 4 | |a Immunofluorescence | |
| 650 | 4 | |a Lysozyme inhibitor | |
| 650 | 4 | |a Membrane permeabilization | |
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