Clinical significance of Bacteroides fragilis as a potential prognostic factor in colorectal cancer
Copyright © 2023 Elsevier Ltd. All rights reserved..
INTRODUCTION: Bacteroides fragilis (B. fragilis) is considered to act in an anti-inflammatory manner on the intestinal tract. On the contrary, enterotoxigenic B. fragilis (ETBF), a subtype of B. fragilis, produces an enterotoxin (BFT; B. fragilis toxin), leading to asymptomatic chronic infections and colonic tumor formation. However, the impact of B. fragilis and ETBF on the clinical outcome of colorectal cancer (CRC) remains unclear. We aim to assess whether their presence affects the outcome in patients with CRC after curative resection.
METHODS: We obtained 197 pairs of matched formalin-fixed paraffin-embedded samples from cancerous and adjacent non-cancerous tissues of patients with pathological stage (pstage) II and III CRC after curative resection. The presence of B. fragilis and ETBF were estimated using real-time polymerase chain reaction, and recurrence-free survival (RFS) and overall survival (OS) of the patients were analyzed.
RESULTS: 16S rRNA for B. fragilis and bft DNA were detected in 120 (60.9%) and 12 (6.1%) of the 197 patients, respectively. B. fragilis-positive patients had better RFS than B. fragilis-negative patients, although that was not statistically significant. In subgroup analysis, better outcomes on RFS were observed in the presence of B. fragilis in pstage II and left-sided CRC. The association of B. fragilis positivity on OS was accentuated in the depth of T4 subgroup. No significant differences were observed in RFS and OS between ETBF and non-toxigenic B. fragilis.
CONCLUSIONS: Our findings suggest that the presence of B. fragilis is associated with better outcomes in patients with pstage II and III CRC after curative resection.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:84 |
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| Enthalten in: |
Anaerobe - 84(2023) vom: 07. Dez., Seite 102784 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Matsumiya, Yuriko [VerfasserIn] |
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| Links: |
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| Themen: |
Bacterial DNA |
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| Anmerkungen: |
Date Completed 18.12.2023 Date Revised 18.12.2023 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.anaerobe.2023.102784 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM363002286 |
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| 245 | 1 | 0 | |a Clinical significance of Bacteroides fragilis as a potential prognostic factor in colorectal cancer |
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| 500 | |a Date Revised 18.12.2023 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2023 Elsevier Ltd. All rights reserved. | ||
| 520 | |a INTRODUCTION: Bacteroides fragilis (B. fragilis) is considered to act in an anti-inflammatory manner on the intestinal tract. On the contrary, enterotoxigenic B. fragilis (ETBF), a subtype of B. fragilis, produces an enterotoxin (BFT; B. fragilis toxin), leading to asymptomatic chronic infections and colonic tumor formation. However, the impact of B. fragilis and ETBF on the clinical outcome of colorectal cancer (CRC) remains unclear. We aim to assess whether their presence affects the outcome in patients with CRC after curative resection | ||
| 520 | |a METHODS: We obtained 197 pairs of matched formalin-fixed paraffin-embedded samples from cancerous and adjacent non-cancerous tissues of patients with pathological stage (pstage) II and III CRC after curative resection. The presence of B. fragilis and ETBF were estimated using real-time polymerase chain reaction, and recurrence-free survival (RFS) and overall survival (OS) of the patients were analyzed | ||
| 520 | |a RESULTS: 16S rRNA for B. fragilis and bft DNA were detected in 120 (60.9%) and 12 (6.1%) of the 197 patients, respectively. B. fragilis-positive patients had better RFS than B. fragilis-negative patients, although that was not statistically significant. In subgroup analysis, better outcomes on RFS were observed in the presence of B. fragilis in pstage II and left-sided CRC. The association of B. fragilis positivity on OS was accentuated in the depth of T4 subgroup. No significant differences were observed in RFS and OS between ETBF and non-toxigenic B. fragilis | ||
| 520 | |a CONCLUSIONS: Our findings suggest that the presence of B. fragilis is associated with better outcomes in patients with pstage II and III CRC after curative resection | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Bacterial DNA | |
| 650 | 4 | |a Bacteroides fragilis | |
| 650 | 4 | |a Colorectal cancer | |
| 650 | 4 | |a Pathogenic biology | |
| 650 | 4 | |a Real-time polymerase chain reaction | |
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| 700 | 1 | |a Suenaga, Mitsukuni |e verfasserin |4 aut | |
| 700 | 1 | |a Ishikawa, Toshiaki |e verfasserin |4 aut | |
| 700 | 1 | |a Kudo, Toshifumi |e verfasserin |4 aut | |
| 700 | 1 | |a Nakagawa, Tsuyoshi |e verfasserin |4 aut | |
| 700 | 1 | |a Okamoto, Kentaro |e verfasserin |4 aut | |
| 700 | 1 | |a Tokunaga, Masanori |e verfasserin |4 aut | |
| 700 | 1 | |a Hurtado, Claudia |e verfasserin |4 aut | |
| 700 | 1 | |a Yamada, Yuki |e verfasserin |4 aut | |
| 700 | 1 | |a Oka, Kentaro |e verfasserin |4 aut | |
| 700 | 1 | |a Takahashi, Motomichi |e verfasserin |4 aut | |
| 700 | 1 | |a Lopez Kostner, Luis Francisco |e verfasserin |4 aut | |
| 700 | 1 | |a O'Ryan Gallardo, Miguel Luis |e verfasserin |4 aut | |
| 700 | 1 | |a Uetake, Hiroyuki |e verfasserin |4 aut | |
| 700 | 1 | |a Kinugasa, Yusuke |e verfasserin |4 aut | |
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