Phage antibody library technology in tumor therapy : a review
Tumor is a serious threat to human health. At present, surgical resection, chemoradiotherapy, targeted therapy and immunotherapy are the main therapeutic strategies. Monoclonal antibody has gradually become an indispensable drug type in the clinical treatment of cancer due to its high efficiency and low toxicity. Phage antibody library technology (PALT) is a novel monoclonal antibody preparation technique. The recombinant immunoglobulin variable region of heavy chain (VH)/variable region of light chain (VL) gene is integrated into the phage vector, and the antibody is expressed on the phage surface in the form of fusion protein to obtain a diverse antibody library. Through the process of adsorption-elution-amplification, the antibody library can be screened to obtain the antibody molecule with specific binding antigen as well as its gene sequence. PALT has the advantages of short antibody production cycle, strong plasticity of antibody structure, large antibody yield, high diversity and direct production of humanized antibodies. It has been used in screening tumor markers and preparation of antibody drugs for breast cancer, gastric cancer, lung cancer and liver cancer. This article reviews the recent progress and the application of PALT in tumor therapy.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2023 |
|---|---|
| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:39 |
|---|---|
| Enthalten in: |
Sheng wu gong cheng xue bao = Chinese journal of biotechnology - 39(2023), 9 vom: 25. Sept., Seite 3644-3669 |
| Sprache: |
Chinesisch |
|---|
| Beteiligte Personen: |
Chen, Xiaoyang [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
Antibodies, Monoclonal |
|---|
| Anmerkungen: |
Date Completed 09.10.2023 Date Revised 09.10.2023 published: Print Citation Status MEDLINE |
|---|
| doi: |
10.13345/j.cjb.230048 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM36299434X |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM36299434X | ||
| 003 | DE-627 | ||
| 005 | 20231226092414.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231226s2023 xx |||||o 00| ||chi c | ||
| 024 | 7 | |a 10.13345/j.cjb.230048 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1209.xml |
| 035 | |a (DE-627)NLM36299434X | ||
| 035 | |a (NLM)37805844 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a chi | ||
| 100 | 1 | |a Chen, Xiaoyang |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Phage antibody library technology in tumor therapy |b a review |
| 264 | 1 | |c 2023 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 09.10.2023 | ||
| 500 | |a Date Revised 09.10.2023 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Tumor is a serious threat to human health. At present, surgical resection, chemoradiotherapy, targeted therapy and immunotherapy are the main therapeutic strategies. Monoclonal antibody has gradually become an indispensable drug type in the clinical treatment of cancer due to its high efficiency and low toxicity. Phage antibody library technology (PALT) is a novel monoclonal antibody preparation technique. The recombinant immunoglobulin variable region of heavy chain (VH)/variable region of light chain (VL) gene is integrated into the phage vector, and the antibody is expressed on the phage surface in the form of fusion protein to obtain a diverse antibody library. Through the process of adsorption-elution-amplification, the antibody library can be screened to obtain the antibody molecule with specific binding antigen as well as its gene sequence. PALT has the advantages of short antibody production cycle, strong plasticity of antibody structure, large antibody yield, high diversity and direct production of humanized antibodies. It has been used in screening tumor markers and preparation of antibody drugs for breast cancer, gastric cancer, lung cancer and liver cancer. This article reviews the recent progress and the application of PALT in tumor therapy | ||
| 650 | 4 | |a Review | |
| 650 | 4 | |a English Abstract | |
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a markers | |
| 650 | 4 | |a monoclonal antibody | |
| 650 | 4 | |a phage antibody library technology | |
| 650 | 4 | |a targeted therapy | |
| 650 | 4 | |a tumor | |
| 650 | 7 | |a Immunoglobulin Variable Region |2 NLM | |
| 650 | 7 | |a Antibodies, Monoclonal |2 NLM | |
| 650 | 7 | |a Peptide Library |2 NLM | |
| 700 | 1 | |a An, Ruiheng |e verfasserin |4 aut | |
| 700 | 1 | |a Huang, Ju |e verfasserin |4 aut | |
| 700 | 1 | |a Liang, Youfeng |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Wenjing |e verfasserin |4 aut | |
| 700 | 1 | |a Hao, Mingxuan |e verfasserin |4 aut | |
| 700 | 1 | |a Guo, Rui |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Xiaoning |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Yongchao |e verfasserin |4 aut | |
| 700 | 1 | |a Ying, Lu |e verfasserin |4 aut | |
| 700 | 1 | |a Yang, Zhao |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Sheng wu gong cheng xue bao = Chinese journal of biotechnology |d 1997 |g 39(2023), 9 vom: 25. Sept., Seite 3644-3669 |w (DE-627)NLM093700067 |x 1872-2075 |7 nnns |
| 773 | 1 | 8 | |g volume:39 |g year:2023 |g number:9 |g day:25 |g month:09 |g pages:3644-3669 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.13345/j.cjb.230048 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 39 |j 2023 |e 9 |b 25 |c 09 |h 3644-3669 | ||