Impact of time of culture specimen collection on the recovery of Neisseria gonorrhoeae after a positive nucleic acid amplification test
© Author(s) (or their employer(s)) 2023. No commercial re-use. See rights and permissions. Published by BMJ..
OBJECTIVES: Culture of Neisseria gonorrhoeae remains essential for antimicrobial resistance (AMR) surveillance. We evaluated the effect of time of specimen collection on culture yield following a positive nucleic acid amplification test (NAAT).
METHODS: We retrospectively assessed N. gonorrhoeae culture yield among asymptomatic individuals (largely men who have sex with men) who attended for sexual health screening and had a positive NAAT. Participants underwent either same-day testing and notification (Drassanes Exprés) or standard screening with deferred testing.
RESULTS: Among 10 423 screened individuals, 809 (7.7%) tested positive for N. gonorrhoeae. A total of 995 different anatomical sites of infection culture was performed in 583 of 995 (58.6%) of anatomical sites (Drassanes Exprés 278 of 347, 80.1%; standard screening 305 of 648, 47.1%; p<0.001). Recovery was highest when culture specimens were collected within 3-7 days of screening with only a slight drop in recovery when the interval extended to 7 days . Recovery from pharynx was 38 of 149 (25.5%) within 3 days, 19 of 81 (23.4%) after 4-7 days (p=0.7245), 11 of 102 (10.7%) after 8-14 days (p<0.0036) and 1 of 22 (4.5%) with longer delays (p=0.00287). Recovery from rectum was 49 of 75 (65.3%) within 3 days, 28 of 45 (62.2%) after 4-7 days (p=0.7318), 41 of 69 (59.4%) after 8-14 days (p=0.4651) and 6 of 18 (33.3%) with longer delays (p=0.0131). Median culture specimen collection time was 1 day within Drassanes Exprés vs 8 days within standard screening. Consequently, the overall culture yield was slightly higher within Drassanes Exprés (102/278, 36.6% vs 99/305, 32.5%; p=0.2934).
CONCLUSION: Reducing the interval between screening and collection of culture specimens increased N. gonorrhoeae recovery in extragenital samples. Implementing a same-day testing and notification programme increased collection of culture samples and culture yield in our setting, which may help AMR surveillance.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2023 |
|---|---|
| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:99 |
|---|---|
| Enthalten in: |
Sexually transmitted infections - 99(2023), 8 vom: 01. Dez., Seite 520-526 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Nadal-Barón, Patricia [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
Clinical Laboratory Techniques |
|---|
| Anmerkungen: |
Date Completed 27.11.2023 Date Revised 10.01.2024 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1136/sextrans-2023-055899 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM362962596 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM362962596 | ||
| 003 | DE-627 | ||
| 005 | 20240114233358.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231226s2023 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1136/sextrans-2023-055899 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1255.xml |
| 035 | |a (DE-627)NLM362962596 | ||
| 035 | |a (NLM)37802652 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Nadal-Barón, Patricia |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Impact of time of culture specimen collection on the recovery of Neisseria gonorrhoeae after a positive nucleic acid amplification test |
| 264 | 1 | |c 2023 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 27.11.2023 | ||
| 500 | |a Date Revised 10.01.2024 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © Author(s) (or their employer(s)) 2023. No commercial re-use. See rights and permissions. Published by BMJ. | ||
| 520 | |a OBJECTIVES: Culture of Neisseria gonorrhoeae remains essential for antimicrobial resistance (AMR) surveillance. We evaluated the effect of time of specimen collection on culture yield following a positive nucleic acid amplification test (NAAT) | ||
| 520 | |a METHODS: We retrospectively assessed N. gonorrhoeae culture yield among asymptomatic individuals (largely men who have sex with men) who attended for sexual health screening and had a positive NAAT. Participants underwent either same-day testing and notification (Drassanes Exprés) or standard screening with deferred testing | ||
| 520 | |a RESULTS: Among 10 423 screened individuals, 809 (7.7%) tested positive for N. gonorrhoeae. A total of 995 different anatomical sites of infection culture was performed in 583 of 995 (58.6%) of anatomical sites (Drassanes Exprés 278 of 347, 80.1%; standard screening 305 of 648, 47.1%; p<0.001). Recovery was highest when culture specimens were collected within 3-7 days of screening with only a slight drop in recovery when the interval extended to 7 days . Recovery from pharynx was 38 of 149 (25.5%) within 3 days, 19 of 81 (23.4%) after 4-7 days (p=0.7245), 11 of 102 (10.7%) after 8-14 days (p<0.0036) and 1 of 22 (4.5%) with longer delays (p=0.00287). Recovery from rectum was 49 of 75 (65.3%) within 3 days, 28 of 45 (62.2%) after 4-7 days (p=0.7318), 41 of 69 (59.4%) after 8-14 days (p=0.4651) and 6 of 18 (33.3%) with longer delays (p=0.0131). Median culture specimen collection time was 1 day within Drassanes Exprés vs 8 days within standard screening. Consequently, the overall culture yield was slightly higher within Drassanes Exprés (102/278, 36.6% vs 99/305, 32.5%; p=0.2934) | ||
| 520 | |a CONCLUSION: Reducing the interval between screening and collection of culture specimens increased N. gonorrhoeae recovery in extragenital samples. Implementing a same-day testing and notification programme increased collection of culture samples and culture yield in our setting, which may help AMR surveillance | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Clinical Laboratory Techniques | |
| 650 | 4 | |a Diagnostic Screening Programs | |
| 650 | 4 | |a NEISSERIA GONORRHOEAE | |
| 650 | 4 | |a Nucleic Acid Amplification Techniques | |
| 650 | 4 | |a SCREENING | |
| 700 | 1 | |a Trejo-Zahinos, Jesús |e verfasserin |4 aut | |
| 700 | 1 | |a García, Jorge Nestor |e verfasserin |4 aut | |
| 700 | 1 | |a Salmerón, Paula |e verfasserin |4 aut | |
| 700 | 1 | |a Sulleiro, Elena |e verfasserin |4 aut | |
| 700 | 1 | |a Arando, Maider |e verfasserin |4 aut | |
| 700 | 1 | |a Descalzo, Vicente |e verfasserin |4 aut | |
| 700 | 1 | |a Álvarez-Lopez, Patricia |e verfasserin |4 aut | |
| 700 | 1 | |a El Ouazzani, Rachid |e verfasserin |4 aut | |
| 700 | 1 | |a López, Luis |e verfasserin |4 aut | |
| 700 | 1 | |a Zarzuela, Francesc |e verfasserin |4 aut | |
| 700 | 1 | |a Ruiz, Edurne |e verfasserin |4 aut | |
| 700 | 1 | |a Llinas, Montserrat |e verfasserin |4 aut | |
| 700 | 1 | |a Blanco-Grau, Albert |e verfasserin |4 aut | |
| 700 | 1 | |a Curran, Adrian |e verfasserin |4 aut | |
| 700 | 1 | |a Larrosa, María Nieves |e verfasserin |4 aut | |
| 700 | 1 | |a Pumarola, Tomàs |e verfasserin |4 aut | |
| 700 | 1 | |a Hoyos-Mallecot, Yannick |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Sexually transmitted infections |d 1998 |g 99(2023), 8 vom: 01. Dez., Seite 520-526 |w (DE-627)NLM09574374X |x 1472-3263 |7 nnns |
| 773 | 1 | 8 | |g volume:99 |g year:2023 |g number:8 |g day:01 |g month:12 |g pages:520-526 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1136/sextrans-2023-055899 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 99 |j 2023 |e 8 |b 01 |c 12 |h 520-526 | ||