Pseudomonas aeruginosa AlgF is a protein-protein interaction mediator required for acetylation of the alginate exopolysaccharide
Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved..
Enzymatic modifications of bacterial exopolysaccharides enhance immune evasion and persistence during infection. In the Gram-negative opportunistic pathogen Pseudomonas aeruginosa, acetylation of alginate reduces opsonic killing by phagocytes and improves reactive oxygen species scavenging. Although it is well known that alginate acetylation in P. aeruginosa requires AlgI, AlgJ, AlgF, and AlgX, how these proteins coordinate polymer modification at a molecular level remains unclear. Here, we describe the structural characterization of AlgF and its protein interaction network. We characterize direct interactions between AlgF and both AlgJ and AlgX in vitro and demonstrate an association between AlgF and AlgX, as well as AlgJ and AlgI, in P. aeruginosa. We determine that AlgF does not exhibit acetylesterase activity and is unable to bind to polymannuronate in vitro. Therefore, we propose that AlgF functions to mediate protein-protein interactions between alginate acetylation enzymes, forming the periplasmic AlgJFXK (AlgJ-AlgF-AlgX-AlgK) acetylation and export complex required for robust biofilm formation.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:299 |
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| Enthalten in: |
The Journal of biological chemistry - 299(2023), 11 vom: 20. Nov., Seite 105314 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Low, Kristin E [VerfasserIn] |
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| Links: |
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| Themen: |
Acetylation |
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| Anmerkungen: |
Date Completed 29.11.2023 Date Revised 06.02.2024 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.jbc.2023.105314 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM362913420 |
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| 100 | 1 | |a Low, Kristin E |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Pseudomonas aeruginosa AlgF is a protein-protein interaction mediator required for acetylation of the alginate exopolysaccharide |
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| 500 | |a published: Print-Electronic | ||
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| 520 | |a Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved. | ||
| 520 | |a Enzymatic modifications of bacterial exopolysaccharides enhance immune evasion and persistence during infection. In the Gram-negative opportunistic pathogen Pseudomonas aeruginosa, acetylation of alginate reduces opsonic killing by phagocytes and improves reactive oxygen species scavenging. Although it is well known that alginate acetylation in P. aeruginosa requires AlgI, AlgJ, AlgF, and AlgX, how these proteins coordinate polymer modification at a molecular level remains unclear. Here, we describe the structural characterization of AlgF and its protein interaction network. We characterize direct interactions between AlgF and both AlgJ and AlgX in vitro and demonstrate an association between AlgF and AlgX, as well as AlgJ and AlgI, in P. aeruginosa. We determine that AlgF does not exhibit acetylesterase activity and is unable to bind to polymannuronate in vitro. Therefore, we propose that AlgF functions to mediate protein-protein interactions between alginate acetylation enzymes, forming the periplasmic AlgJFXK (AlgJ-AlgF-AlgX-AlgK) acetylation and export complex required for robust biofilm formation | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Pseudomonas aeruginosa | |
| 650 | 4 | |a acetylation | |
| 650 | 4 | |a alginate | |
| 650 | 4 | |a biofilms | |
| 650 | 4 | |a exopolysaccharide | |
| 650 | 7 | |a Alginates |2 NLM | |
| 650 | 7 | |a Bacterial Proteins |2 NLM | |
| 650 | 7 | |a algF protein, Pseudomonas aeruginosa |2 NLM | |
| 700 | 1 | |a Gheorghita, Andreea A |e verfasserin |4 aut | |
| 700 | 1 | |a Tammam, Stephanie D |e verfasserin |4 aut | |
| 700 | 1 | |a Whitfield, Gregory B |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Yancheng E |e verfasserin |4 aut | |
| 700 | 1 | |a Riley, Laura M |e verfasserin |4 aut | |
| 700 | 1 | |a Weadge, Joel T |e verfasserin |4 aut | |
| 700 | 1 | |a Caldwell, Shane J |e verfasserin |4 aut | |
| 700 | 1 | |a Chong, P Andrew |e verfasserin |4 aut | |
| 700 | 1 | |a Walvoort, Marthe T C |e verfasserin |4 aut | |
| 700 | 1 | |a Kitova, Elena N |e verfasserin |4 aut | |
| 700 | 1 | |a Klassen, John S |e verfasserin |4 aut | |
| 700 | 1 | |a Codée, Jeroen D C |e verfasserin |4 aut | |
| 700 | 1 | |a Howell, P Lynne |e verfasserin |4 aut | |
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