Lercanidipine distribution in warm-blooded animals
THE AIM OF THE STUDY: Is to investigate the lercadipine distribution in warm-blooded animals (rats). The experimental study used rats of Wistar race. TLC, GC-MS and UV-spectrophotometry methods were used for physical and chemical analysis. Semilethal (890 mg/kg) dose of lercadipine, previously suspended in water, was injected into stomach of laboratory animals. Examined substance was isolated from the thick tissues and animals' blood by acetone, cleaned with a change of solvent and macrocolumn chromatography using 30 µm «Silasorb S-18» sorbent and acetonitrile-water (8:2) polar eluent. The analyte was identified by chromatographic behavior in the thin sorbent layer, retention time and set of positive ions in its mass spectrum, as well as by UV-spectrum. The analyte was determined quantitatively in bioactive matrix using UV-spectrophotometry. The methods were validated by criteria of linearity, selectivity, accuracy, precision, detection limits and quantitative determination. The main content of lercanidipine (mg/100 g) was determined in the stomach content (198.183±29.541), the stomach (195.312±21.579), the small intestine (47.096±3.947), the spleen (38.952±3.532) and the liver (26.211±2.232).
Medienart: |
E-Artikel |
---|
Erscheinungsjahr: |
2023 |
---|---|
Erschienen: |
2023 |
Enthalten in: |
Zur Gesamtaufnahme - volume:66 |
---|---|
Enthalten in: |
Sudebno-meditsinskaia ekspertiza - 66(2023), 5 vom: 05., Seite 47-52 |
Sprache: |
Russisch |
---|
Weiterer Titel: |
Raspredelenie lerkanidipina v organizme teplokrovnykh zhivotnykh |
---|
Beteiligte Personen: |
Kvachakhiya, L L [VerfasserIn] |
---|
Links: |
---|
Themen: |
059QF0KO0R |
---|
Anmerkungen: |
Date Completed 01.11.2023 Date Revised 01.11.2023 published: Print Citation Status MEDLINE |
---|
doi: |
10.17116/sudmed20236605147 |
---|
funding: |
|
---|---|
Förderinstitution / Projekttitel: |
|
PPN (Katalog-ID): |
NLM362901813 |
---|
LEADER | 01000naa a22002652 4500 | ||
---|---|---|---|
001 | NLM362901813 | ||
003 | DE-627 | ||
005 | 20231226092217.0 | ||
007 | cr uuu---uuuuu | ||
008 | 231226s2023 xx |||||o 00| ||rus c | ||
024 | 7 | |a 10.17116/sudmed20236605147 |2 doi | |
028 | 5 | 2 | |a pubmed24n1209.xml |
035 | |a (DE-627)NLM362901813 | ||
035 | |a (NLM)37796461 | ||
040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
041 | |a rus | ||
100 | 1 | |a Kvachakhiya, L L |e verfasserin |4 aut | |
245 | 1 | 0 | |a Lercanidipine distribution in warm-blooded animals |
246 | 3 | 3 | |a Raspredelenie lerkanidipina v organizme teplokrovnykh zhivotnykh |
264 | 1 | |c 2023 | |
336 | |a Text |b txt |2 rdacontent | ||
337 | |a ƒaComputermedien |b c |2 rdamedia | ||
338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
500 | |a Date Completed 01.11.2023 | ||
500 | |a Date Revised 01.11.2023 | ||
500 | |a published: Print | ||
500 | |a Citation Status MEDLINE | ||
520 | |a THE AIM OF THE STUDY: Is to investigate the lercadipine distribution in warm-blooded animals (rats). The experimental study used rats of Wistar race. TLC, GC-MS and UV-spectrophotometry methods were used for physical and chemical analysis. Semilethal (890 mg/kg) dose of lercadipine, previously suspended in water, was injected into stomach of laboratory animals. Examined substance was isolated from the thick tissues and animals' blood by acetone, cleaned with a change of solvent and macrocolumn chromatography using 30 µm «Silasorb S-18» sorbent and acetonitrile-water (8:2) polar eluent. The analyte was identified by chromatographic behavior in the thin sorbent layer, retention time and set of positive ions in its mass spectrum, as well as by UV-spectrum. The analyte was determined quantitatively in bioactive matrix using UV-spectrophotometry. The methods were validated by criteria of linearity, selectivity, accuracy, precision, detection limits and quantitative determination. The main content of lercanidipine (mg/100 g) was determined in the stomach content (198.183±29.541), the stomach (195.312±21.579), the small intestine (47.096±3.947), the spleen (38.952±3.532) and the liver (26.211±2.232) | ||
650 | 4 | |a English Abstract | |
650 | 4 | |a Journal Article | |
650 | 4 | |a chemical toxicology screening | |
650 | 4 | |a distribution in vivo | |
650 | 4 | |a lercanidipine | |
650 | 4 | |a warm-blooded animals | |
650 | 7 | |a lercanidipine |2 NLM | |
650 | 7 | |a V7XTJ4R0BH |2 NLM | |
650 | 7 | |a Dihydropyridines |2 NLM | |
650 | 7 | |a Water |2 NLM | |
650 | 7 | |a 059QF0KO0R |2 NLM | |
700 | 1 | |a Shormanov, V K |e verfasserin |4 aut | |
773 | 0 | 8 | |i Enthalten in |t Sudebno-meditsinskaia ekspertiza |d 1962 |g 66(2023), 5 vom: 05., Seite 47-52 |w (DE-627)NLM00014312X |x 0039-4521 |7 nnns |
773 | 1 | 8 | |g volume:66 |g year:2023 |g number:5 |g day:05 |g pages:47-52 |
856 | 4 | 0 | |u http://dx.doi.org/10.17116/sudmed20236605147 |3 Volltext |
912 | |a GBV_USEFLAG_A | ||
912 | |a GBV_NLM | ||
951 | |a AR | ||
952 | |d 66 |j 2023 |e 5 |b 05 |h 47-52 |