PAM-flexible genome editing with an engineered chimeric Cas9
© 2023. Springer Nature Limited..
CRISPR enzymes require a defined protospacer adjacent motif (PAM) flanking a guide RNA-programmed target site, limiting their sequence accessibility for robust genome editing applications. In this study, we recombine the PAM-interacting domain of SpRY, a broad-targeting Cas9 possessing an NRN > NYN (R = A or G, Y = C or T) PAM preference, with the N-terminus of Sc + +, a Cas9 with simultaneously broad, efficient, and accurate NNG editing capabilities, to generate a chimeric enzyme with highly flexible PAM preference: SpRYc. We demonstrate that SpRYc leverages properties of both enzymes to specifically edit diverse PAMs and disease-related loci for potential therapeutic applications. In total, the approaches to generate SpRYc, coupled with its robust flexibility, highlight the power of integrative protein design for Cas9 engineering and motivate downstream editing applications that require precise genomic positioning.
| Errataetall: | |
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| Medienart: |
E-Artikel |
| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:14 |
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| Enthalten in: |
Nature communications - 14(2023), 1 vom: 04. Okt., Seite 6175 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Zhao, Lin [VerfasserIn] |
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| Links: |
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| Themen: |
CRISPR-Associated Protein 9 |
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| Anmerkungen: |
Date Completed 06.10.2023 Date Revised 10.02.2024 published: Electronic UpdateOf: Res Sq. 2023 Mar 07;:. - PMID 36945419 Citation Status MEDLINE |
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| doi: |
10.1038/s41467-023-41829-y |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM36287767X |
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| 245 | 1 | 0 | |a PAM-flexible genome editing with an engineered chimeric Cas9 |
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| 520 | |a CRISPR enzymes require a defined protospacer adjacent motif (PAM) flanking a guide RNA-programmed target site, limiting their sequence accessibility for robust genome editing applications. In this study, we recombine the PAM-interacting domain of SpRY, a broad-targeting Cas9 possessing an NRN > NYN (R = A or G, Y = C or T) PAM preference, with the N-terminus of Sc + +, a Cas9 with simultaneously broad, efficient, and accurate NNG editing capabilities, to generate a chimeric enzyme with highly flexible PAM preference: SpRYc. We demonstrate that SpRYc leverages properties of both enzymes to specifically edit diverse PAMs and disease-related loci for potential therapeutic applications. In total, the approaches to generate SpRYc, coupled with its robust flexibility, highlight the power of integrative protein design for Cas9 engineering and motivate downstream editing applications that require precise genomic positioning | ||
| 650 | 4 | |a Journal Article | |
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| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
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| 700 | 1 | |a Koseki, Sabrina R T |e verfasserin |4 aut | |
| 700 | 1 | |a Silverstein, Rachel A |e verfasserin |4 aut | |
| 700 | 1 | |a Amrani, Nadia |e verfasserin |4 aut | |
| 700 | 1 | |a Peng, Christina |e verfasserin |4 aut | |
| 700 | 1 | |a Kramme, Christian |e verfasserin |4 aut | |
| 700 | 1 | |a Savic, Natasha |e verfasserin |4 aut | |
| 700 | 1 | |a Pacesa, Martin |e verfasserin |4 aut | |
| 700 | 1 | |a Rodríguez, Tomás C |e verfasserin |4 aut | |
| 700 | 1 | |a Stan, Teodora |e verfasserin |4 aut | |
| 700 | 1 | |a Tysinger, Emma |e verfasserin |4 aut | |
| 700 | 1 | |a Hong, Lauren |e verfasserin |4 aut | |
| 700 | 1 | |a Yudistyra, Vivian |e verfasserin |4 aut | |
| 700 | 1 | |a Ponnapati, Manvitha R |e verfasserin |4 aut | |
| 700 | 1 | |a Jacobson, Joseph M |e verfasserin |4 aut | |
| 700 | 1 | |a Church, George M |e verfasserin |4 aut | |
| 700 | 1 | |a Jakimo, Noah |e verfasserin |4 aut | |
| 700 | 1 | |a Truant, Ray |e verfasserin |4 aut | |
| 700 | 1 | |a Jinek, Martin |e verfasserin |4 aut | |
| 700 | 1 | |a Kleinstiver, Benjamin P |e verfasserin |4 aut | |
| 700 | 1 | |a Sontheimer, Erik J |e verfasserin |4 aut | |
| 700 | 1 | |a Chatterjee, Pranam |e verfasserin |4 aut | |
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