Anxiolytic-like effects of Pseudospondias microcarpa hydroethanolic leaf extract in zebrafish : Possible involvement of GABAergic and serotonergic pathways
© 2023. Kunming Institute of Botany, CAS..
Pseudospondias microcarpa is used in ethnomedicine to manage central nervous system diseases. The hydroethanolic extract (PME) from the leaves of the plant has shown anxiolytic-like properties in mice anxiety models. However, its effects in chronic anxiety models and possible mechanism(s) of action were not studied. Therefore, the current study evaluated the anxiolytic-like mechanisms of PME in zebrafish models of anxiety. The zebrafish light dark test (LDT) and novel tank test (NTT) were employed to assess the anxiolytic-like effects of PME (0.1, 0.3, 1.0 mg mL-1), fluoxetine (3 × 10-5 mg mL-1) and diazepam (1.5 × 10-7 mg mL-1). The chronic unpredictable stress (CUS) test was used to further evaluate the extract's anxiolytic-like properties. The potential mechanisms of anxiolytic action of the extract was evaluated after pre-treated with flumazenil, granisetron, methysergide, or pizotifen, all at 1 × 10-3 mg mL-1. The extract significantly decreased anxiety behaviours in the NT and LD tests. These observed effects of the extract were however counteracted by flumazenil, granisetron, methysergide and pizotifen pre-treatment. In addition, PME treatment significantly reversed CUS-induced anxiety behaviours in zebrafish. Results show that PME possesses anxiolytic-like effects possibly through interaction with serotonergic and gamma-aminobutyric acid mediated pathways.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:13 |
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| Enthalten in: |
Natural products and bioprospecting - 13(2023), 1 vom: 04. Okt., Seite 33 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Adongo, Donatus Wewura [VerfasserIn] |
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| Links: |
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| Themen: |
Anxiety disorders |
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| Anmerkungen: |
Date Revised 21.11.2023 published: Electronic Citation Status PubMed-not-MEDLINE |
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| doi: |
10.1007/s13659-023-00399-8 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM362829780 |
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| 520 | |a Pseudospondias microcarpa is used in ethnomedicine to manage central nervous system diseases. The hydroethanolic extract (PME) from the leaves of the plant has shown anxiolytic-like properties in mice anxiety models. However, its effects in chronic anxiety models and possible mechanism(s) of action were not studied. Therefore, the current study evaluated the anxiolytic-like mechanisms of PME in zebrafish models of anxiety. The zebrafish light dark test (LDT) and novel tank test (NTT) were employed to assess the anxiolytic-like effects of PME (0.1, 0.3, 1.0 mg mL-1), fluoxetine (3 × 10-5 mg mL-1) and diazepam (1.5 × 10-7 mg mL-1). The chronic unpredictable stress (CUS) test was used to further evaluate the extract's anxiolytic-like properties. The potential mechanisms of anxiolytic action of the extract was evaluated after pre-treated with flumazenil, granisetron, methysergide, or pizotifen, all at 1 × 10-3 mg mL-1. The extract significantly decreased anxiety behaviours in the NT and LD tests. These observed effects of the extract were however counteracted by flumazenil, granisetron, methysergide and pizotifen pre-treatment. In addition, PME treatment significantly reversed CUS-induced anxiety behaviours in zebrafish. Results show that PME possesses anxiolytic-like effects possibly through interaction with serotonergic and gamma-aminobutyric acid mediated pathways | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Anxiety disorders | |
| 650 | 4 | |a Benzodiazepines | |
| 650 | 4 | |a Novel tank | |
| 650 | 4 | |a Pseudospondias microcarpa | |
| 650 | 4 | |a Zebrafish | |
| 700 | 1 | |a Benneh, Charles Kwaku |e verfasserin |4 aut | |
| 700 | 1 | |a Tandoh, Augustine |e verfasserin |4 aut | |
| 700 | 1 | |a Biney, Robert Peter |e verfasserin |4 aut | |
| 700 | 1 | |a Kukuia, Kennedy Kwami Edem |e verfasserin |4 aut | |
| 700 | 1 | |a Mante, Priscilla Kolibea |e verfasserin |4 aut | |
| 700 | 1 | |a Harley, Benjamin Kingsley |e verfasserin |4 aut | |
| 700 | 1 | |a Oteng, David |e verfasserin |4 aut | |
| 700 | 1 | |a Appiah, Emmanuel Aduboffour |e verfasserin |4 aut | |
| 700 | 1 | |a Anorbor, Ernest Cudjoe |e verfasserin |4 aut | |
| 700 | 1 | |a Woode, Eric |e verfasserin |4 aut | |
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